Labshake search
Citations for GE Life Sciences :
1001 - 1050 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by either Protein A affinity or by using ion exchange chromatography (MonoQ 10/100 GL column, GE Healthcare) with a 0 mM–1 M NaCl gradient ...
-
bioRxiv - Biochemistry 2020Quote: ... and a two-step fast protein liquid chromatography (FPLC) purification protocol using an ÄKTA Protein Purification System (GE Healthcare Life Sciences) at 8 °C was then followed to obtain the recombinant protein ...
-
bioRxiv - Immunology 2022Quote: ... The culture was harvested after seven days and the supernatant purified into PBS by Protein G affinity using a HiTrap Protein G HP affinity column (GE Healthcare), eluted with 0.1M acetic acid (VWR international ...
-
bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
-
bioRxiv - Microbiology 2021Quote: ... Expression of the recombinant proteins was performed using the ExpiCHO Expression system (Thermoscientific) following purification using HiTrap Protein-A column (GE Healthcare). The purified protein was sterile-filtered and stored in PBS.
-
bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
-
bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
-
bioRxiv - Immunology 2021Quote: ... The ACE2-Ig and RBD-Ig fusion proteins secreted to the medium were purified on HiTrap Protein G High Performance column (Cat#GE17-0405-01, GE Healthcare). Control-Ig was one of the following fusion proteins ...
-
bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing the untagged protein were pooled and the homogenous protein was finally isolated by size-exclusion chromatography onto a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes/NaOH ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
-
bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
-
bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
-
bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare). To detect the deGRadFP expression from the hb-deGRadFP construct ...
-
bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
-
bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
-
bioRxiv - Pathology 2021Quote: ... horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare). Each signal was detected using Immunostar Zeta or LD (Fuji film ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell Signalling) and horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (respectively NA931V and NA934V. 1:10.000. GE Healthcare). Mineral dissolution activity of Oc was measured as described (Maurin et al. ...
-
bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
-
bioRxiv - Cell Biology 2019Quote: ... Membranes were washed three times in TBST before incubation with HRP-conjugated secondary antibodies for 1hr at room temperature in 2% milk TBST (1/10,000, GE Healthcare).
-
bioRxiv - Developmental Biology 2019Quote: ... the membrane was incubated with an appropriate secondary antibody (anti-rabbit or anti-mouse IgG HRP-conjugated Ab (1:10,000 in 3% skim milk in TBS, GE Healthcare), mouse TrueBlot ULTRA ...
-
bioRxiv - Genetics 2019Quote: ... we used the anti-rabbit HRP-conjugated antibody (1/5000 dilutions) with the ECL Western Blotting Detection System (GE Healthcare) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: ... non-fat dry milk in TBST for 1 hr at room temperature: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
-
bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
-
bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed three times for five minutes with 0.1% PBS-Tween20 and incubated for 1 hour at room temperature with anti-rabbit HRP secondary antibody (GE healthcare) in blocking solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
-
bioRxiv - Microbiology 2020Quote: ... The membranes were further incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA) in PBST-BSA for 1 h ...
-
bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
-
bioRxiv - Molecular Biology 2021Quote: ... The membranes were soaked with 1:5000-diluted HRP-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) in TBST containing 5% skim milk for 30 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
-
bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
-
bioRxiv - Microbiology 2024Quote: ... non-fat dry milk in TBS-T for 1 h at room temperature in a rocking platform: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 NA934 and NA931 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
-
bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (GE Healthcare) were used at 1:5,000 for 1 h at room temperature ...
-
bioRxiv - Plant Biology 2021Quote: ... the supernatant was loaded onto a HisTrap HP 1-mL affinity column and eluted using a gradient of 10 to 500 mM imidazole in buffer B using an ÄKTA start FPLC module (GE Healthcare, Uppsala, Sweden). Fractions were analyzed by SDS–polyacrylamide gel electrophoresis ...
-
bioRxiv - Bioengineering 2022Quote: We deployed vendor-neutral pulse sequences developed in RTHawk v3.0.0 (rc4-28-ge3540dda19) (HeartVista Inc., CA, USA) on three 3T systems: (G1) GE Discovery 750 software version DV25 (R02_1549.b) (GE Healthcare, Milwaukee, MI, USA), (S1 ...
-
bioRxiv - Biochemistry 2020Quote: ... and fractions from 15 % to 35 % of buffer B were collected and applied to 5ml HisTrap HP column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer C (20 mM Tris (pH 7.6) ...
-
bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
-
bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
-
bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
-
bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
-
bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
-
bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
-
bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
-
bioRxiv - Biophysics 2022Quote: ... The fusion protein was purified by fast protein liquid chromatography (FPLC) using a 5ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5M imidazole gradient (SKU I5513-100G ...