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Citations for GE Life Sciences :
1151 - 1200 of 1348 citations for 6 cyclopropylpyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 3-fold with R buffer and loaded onto a HiTrap SP column (GE Healthcare). Rrp1 eluted at around 600 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... zGDNFmat -zGFRα1D1-3 was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM Tris (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).
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bioRxiv - Immunology 2020Quote: Buffy coats were diluted [1:3] with PBS and added to Leukosep tubes containing Ficoll-Paque (GE Healthcare). Tubes were centrifuged at 450g for 40 minutes and the resulting buffy layer extracted ...
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bioRxiv - Neuroscience 2020Quote: ... age range: 20-29 years) were scanned in a 3-Tesla MRI scanner (DISCOVERY MR750, GE Healthcare, USA) using a 32-channel head-coil ...
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bioRxiv - Immunology 2020Quote: Whole-blood leukocyte DNA was extracted using the Nucleon BACC 3 DNA extraction kit (GE healthcare, Buckinghamshire, UK). We genotyped patients using the Illumina OmniExpressExome-8 Bead Chip (Illumina ...
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bioRxiv - Genomics 2022Quote: ... with an anti-rabbit IgG (goat polyclonal, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4301, RRID:AB_2650489) secondary antibody was used as a loading control ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Immunology 2023Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
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bioRxiv - Biochemistry 2019Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tagged proteins were filtered on a Superdex 200 10/300 GL or Superose 6 Increase 10/300 GL column (GE Healthcare, Little Chalfont, UK) and then labeled with SNAP-surface-549 (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified extract was either directly loaded onto Superose 6 column following pre-clearing with 150 μl of Sepharose CL-2B (GE Healthcare, 17-0140-01) or subjected to Halo purification by incubating with 100 μl of HaloLink™ Resin (Promega ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... The caveolin-containing eluate was concentrated and further separated by size exclusion chromatography using a Superpose 6 Increase 10/300 GL column (GE Healthcare, Boston, MA, USA) in buffer containing 200 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... sensorchip in HBS-EP buffer (10 mM Hepes pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% surfactant P20) (GE Healthcare). All immobilization steps were performed at a flow rate of 2 µl/min with a final concentration of 100 µg/ml.
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: Full-length mouse Csk (WT, H21I, K43D, and K222R3) were subcloned into a pGEX-6P-3 vector (GE Healthcare) containing an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Samples were first fully submerged in 3 mL of Ficoll-Plaque Plus endotoxin-free water (GE Healthcare, Uppsala, Sweden) in either a 6-well culture plate or 3-mL test tube for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... was analysed at a constant temperature of 25°C under continuous flow of HBS-PE buffer (10mM HEPES pH 7.4, 3 mM EDTA, 0.005% (w/v) Surfactant P20 (GE Healthcare) at 30μl/min (sufficient to prevent mass transfer effects ...
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bioRxiv - Neuroscience 2020Quote: Resting-state fMRI data were collected on a whole-body 3 Tesla MR750 MRI scanner (GE Healthcare, Milwaukee, WI) with an 8-channel receive-only head array coil at the Laureate Institute of Brain Research ...
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bioRxiv - Evolutionary Biology 2019Quote: ... in 3 µL of water and spotted onto indicating Whatman FTA Classic Indicating cards (GE Healthcare Life Sciences, UK) using a micropipette ...
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bioRxiv - Immunology 2020Quote: ... 3-fold serial dilution (spanning a range of 1 μM to 1.4 nM) in HBS-EP buffer (GE healthcare). A high flow rate of 40 μL/min was used ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature with HBS-EP+ running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). IgGs were immobilized on CM5 chips (GE Healthcare # 29104988 ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... acute- and chronic-type ATL patients with 3% dextran and the density gradient medium Ficoll-Paque Plus (GE Healthcare). For lymphoma-type ATL ...
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bioRxiv - Neuroscience 2022Quote: ... and an aliquot of synaptosomal suspension (2 ml) was placed onto a 3-ml Percoll discontinuous gradient (GE Healthcare) containing (in mM ...
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bioRxiv - Plant Biology 2023Quote: 3-dimensional Structured Illumination Microscopy (3D-SIM) and confocal images were acquired on a DeltaVision OMX Blaze (GE Healthcare) and LSM-Zeiss 710 respectively ...
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bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Neuroscience 2023Quote: Both structural and functional MRI data were obtained using a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI). High-resolution T1-weighted structural images were captured using a three-dimensional brain-volume sequence (TE = 3.18 ms ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 3 days post transfection and purified using GraviTrap TALON columns (GE Healthcare # GE29-0005-94). The columns were first equilibrated with purification buffer (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...