Labshake search
Citations for GE Life Sciences :
1101 - 1150 of 1348 citations for 6 cyclopropylpyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... The eluent was concentrated and further purified by using a size-exclusion chromatography (SEC) column (Superose 6 increase 10/300 GL, GE Healthcare, USA) in 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biochemistry 2021Quote: ... The samples (equivalent to ∼85 µg microsomes) were then analyzed with an HPLC system and injected to a Superose 6 column (GE Life Sciences), equilibrated in a running buffer containing 25 mM Tris pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: The protein was purified from filtered cell supernatants using Strep-Tactin resin (IBA) before being subjected to additional purification by gel filtration chromatography using a Superose 6 10/300 column (GE Healthcare, USA) in 1 × PBS ...
-
bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
-
bioRxiv - Biophysics 2022Quote: ... Sample homogeneity was investigated by analytical size exclusion chromatography on a Superose® 6 10/300 GL gel filtration column (GE Healthcare). Aliquots of purified EFL at 0.4 mg/mL were flash frozen in liquid nitrogen and stored at −70 °C.
-
bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
-
bioRxiv - Genetics 2023Quote: ... GST-Clr4 and MBP-Clr6 were expressed in Escherichia coli BL21 (DE3) cells and purified on Ni+ SepharoseTM 6 Fast Flow (for 6His fusions; GE Healthcare, 17531806), Glutathione SepharoseTM 4B (for GST fusions ...
-
bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was subjected to further size-exclusion chromatography (SEC) by a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare, USA) using a flow rate of 0.3 mL/min and a running buffer containing 20 mM HEPES pH 7.5 ...
-
bioRxiv - Physiology 2023Quote: Plasma samples (200 μL) were loaded onto AKTA pure FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare). Lipoproteins were eluted with PBS and 0.25 mL fractions were collected at a flow rate of 0.4 mL/min.
-
bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
-
bioRxiv - Biochemistry 2023Quote: Oligomerization of ClpL WT and mutants was investigated by size exclusion chromatography (SEC) using a Superose® 6 10/300 GL column (GE Healthcare) at 4°C in assay buffer supplemented with 2 mM ATP ...
-
bioRxiv - Genetics 2024Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2024Quote: SEC-MALS experiments were performed using and Ä KTA purifier system connected to a Superose 6 Increase 10/300 GL column (GE healthcare) at a flow rate of 0.4 ml/min in running buffer (100 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
-
bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
-
bioRxiv - Genomics 2020Quote: ... Membranes were washed 3 times in TBST and incubated with Amersham ECL PLUS™ (GE Healthcare, RPN2132) prior to exposure to Super RX film (Fujifilm ...
-
bioRxiv - Immunology 2019Quote: ... Columns were prepared by coupling Y-3 and B22-249 antibodies to CNBr-activated Sepharose (GE Healthcare) (1 mg antibody/40 mg Sepharose) ...
-
bioRxiv - Immunology 2021Quote: ... 2-D and 3-D images were obtained and analyzed using the software program MicroView (GE Healthcare).
-
bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
-
bioRxiv - Neuroscience 2021Quote: The study was performed using a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
-
bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
-
bioRxiv - Neuroscience 2022Quote: The study was performed using a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
-
bioRxiv - Immunology 2020Quote: ... Post-nuclear supernatants were incubated with 3 μg Protein A Sepharose CL-4B (PAS; Ge HealthCare, #GEH17078001) for 1 hour and then with 3 μg PAS plus primary antibody (0.4 ug anti-GFP mAb ...
-
bioRxiv - Neuroscience 2023Quote: ... (3) Size exclusion chromatography (SEC): EVs were isolated from ISF using Sephadex-G25 (GE Healthcare; Uppsala, Sweden) following the vendor’s instruction ...
-
bioRxiv - Neuroscience 2023Quote: MRI scans were performed using a GE 3 Tesla MR750 Discovery scanner (GE Healthcare, Milwaukee WI, USA). The anatomical scan acquired a T1-weighted image with the MPRAGE sequence of TR/TE = 5/2 ms ...
-
bioRxiv - Neuroscience 2024Quote: The experiments were performed in a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
-
bioRxiv - Biophysics 2020Quote: ... The flow-through was then collected and concentrated using a 100 kDa concentrator and further purified on a Superose 6 gel filtration column (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
-
bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... This was performed using an purifier system connected to a Superose 6 Increase 10/300 GL column (both from GE healthcare, Solingen, Germany) at a flow rate of 0.5 ml · min−1 in running buffer (50 mM Tris/HCl (pH 8.0) ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and then incubated with 500-μl of Nickel-Sepharose 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA) for 2 h at 4°C ...
-
C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...
-
bioRxiv - Biochemistry 2022Quote: ... Mpro was eluted with 300 mM imidazole in the lysis buffer and then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size- exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences). We biotinylated the protein with BirA ligase as previously described32.
-
bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
-
bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
-
bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
-
bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
-
bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
-
bioRxiv - Immunology 2020Quote: Purified C105 Fab was incubated with SARS-CoV-2 S trimer at a 2:1 molar ratio per protomer on ice for 30 minutes prior to purification by SEC on a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences). Fab–S complexes were concentrated to 1.6 mg/ml in Tris-buffered saline (TBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
-
bioRxiv - Biophysics 2023Quote: ... The digested protein was concentrated using a 100 kDa concentrator and further purified on a Superose 6 Increase 10/300 gel filtration column (GE Healthcare Life Sciences) equilibrated in an Mg2+-containing buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
-
bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...