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Citations for GE Life Sciences :
1101 - 1150 of 1832 citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Immunology 2020Quote: ... 5-10 μg denaturated proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Antibodies against HOIL1 (H-1 ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were clarified by centrifugation and purified through a 5 mL HisTrap FF column (GE Healthcare), using theÄKTA Pure chromatography system (Cytiva) ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 1 × TBST with 5% non-fat milk: anti-rabbit IgG (1:10,000; NA934V; GE Healthcare) and anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM imidazole and 0.023% DDM) using an ÅKTA purifier FPLC system (GE Healthcare, Chicago, United States). The unbound proteins were eluted from the column with binding buffer until the absorbance at 280 nm reached a stable baseline value ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SE001 was purified from cell supernatants after 5 days of induction using Nickel Excel resin (GE Healthcare), followed by size exclusion chromatography with a Sephadex S200 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted fractions were pooled and loaded onto a pre-equilibrated 5 ml HisTrap HP (GE Healthcare) column ...
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bioRxiv - Plant Biology 2023Quote: ... Fluorescence scanning of acrylamide gels was performed on an Amersham Typhoon 5 scanner (GE Healthcare Life Sciences) using excitation and emission wavelengths of ex488/em520 and ex635/em670 for Cy2 and Cy5 scanning ...
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bioRxiv - Bioengineering 2023Quote: ... A single-use 10 L Cell bag™ bioreactor (working volume: 0.5-5 L; GE Healthcare, USA) was placed on the rocker ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 40 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
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bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates were incubated with 70⍰μl of streptavidin beads (5⍰ml; 17-5113-01, GE Healthcare) at 4° for 3⍰h ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... to a volume of 500 μL and exchanged with a NAP-5 column (GE Healthcare Life Sciences) to a buffer consisting of 20 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was applied to a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), washed with lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was loaded onto the pre-equilibrated HiTrap Q FF 5 mL column (GE Healthcare) at a flow rate of 2.5 mL/min ...
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bioRxiv - Plant Biology 2024Quote: ... Fluorescence scanning of acrylamide gels was performed on an Amersham Typhoon 5 scanner (GE Healthcare Life Sciences) with Cy2 and Cy5 settings using the 488nm and 635nm lasers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The following morning the sample was loaded onto 5 mL HiTrap QFF anion exchange column (GE healthcare) and then washed with 15 CV of buffer A (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... filtered culture supernatant was purified with a 5 mL HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
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bioRxiv - Immunology 2023Quote: ... the cortex was homogenized and resuspended with 5 ml 70% Percoll Plus (GE Healthcare, 17-5445-02) and 5 ml 37% Percoll Plus placed on top ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were then purified by cation exchange using a 5-mL HiTrap SP HP column (GE Healthcare) and the collected fractions analyzed by SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... The reducing agent was removed by passing twice through a NAP-5 desalting column (GE Healthcare, Chicago, IL) equilibrated with G-buffer lacking β-ME ...
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and Sov was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amount of 5-10µg proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Membranes were revealed using a chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μM ZnCl2 and 5 mM β-mercaptoethanol) for XPA and Superdex 200 10/300 increase (GE Healthcare) equilibrated in a different storage buffer (400 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Genomics 2019Quote: ... The diluted supernatants were applied to a 5 mL HiTrap heparin column (HiTrap™ Heparin HP, GE Healthcare) coupled to an ÄKTA purifier system (ÄKTA explorer ...
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bioRxiv - Biophysics 2019Quote: ... The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification was done using an AKTA Purifier FPLC system on a HisTrap FF 5 ml column (GE Healthcare). The column was washed and equilibrated with 10 volumes of LB prior to sample loading ...
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bioRxiv - Biophysics 2020Quote: ... The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Supernatants were filtered by 0.45 μm and loaded onto a 5 mL HiTrap MabSelect SuRe column (GE Healthcare) equilibrated with COP Wash buffer (20 mM Hepes pH 7.4 and 500 mM NaCl) ...
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bioRxiv - Biochemistry 2021Quote: ... 30 min) and the supernatant was loaded onto a 5 mL HisTrap HP Ni Sepharose column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex at reasonable purity were loaded onto a 5 ml HiTrap Heparin column (GE Healthcare), and the bound protein was eluted with a gradient from buffer A to buffer B (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and then loaded on to a HiTrap™ SP Sepharose HP column (5 ml/min, GE Life Sciences) followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B (20 mM HEPES pH 7.5 ...