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Citations for GE Life Sciences :
1001 - 1050 of 1880 citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The lysate soluble fraction was loaded onto a 5 ml HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was clarified by centrifugation and loaded onto HisTrap™ High-Performance 5 mL (GE Healthcare) columns using a peristaltic pump ...
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bioRxiv - Biophysics 2020Quote: ... filtered by 0.22 μm syringe filter and loaded on Ni-NTA (5 mL) column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were then washed as before and incubated for 5 min in ECL Prime (GE Healthcare) before being exposed to films (Thermo Fisher) ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was harvested after 5 days and purified using a MabSelect SuRE Resin (GE Healthcare) followed by size exclusion purification on a Superose 6 Increase column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... A subsequent chromatography step was carried out on a StrepTrap HP 5 ml column (GE Healthcare) in Strep buffers (20 mM MOPS ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified using a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), using wash [0.05 M Tris pH 7.5 ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... sterile filtrated and loaded onto a HisTrap FF 1 ml or 5 ml column (GE Healthcare). Column wash was performed with 4-10 column volumes of binding buffer (25 mM Tris/HCl pH 7.6 ...
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bioRxiv - Cell Biology 2019Quote: ... and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare). Purified CiEB1-mCherry in distilled water was microinjected into the dechorionated Ciona eggs together with AF488 phalloidin solution ...
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bioRxiv - Systems Biology 2019Quote: ... The cleaved proteins were further purified on a 5 mL HiTrap Q HP column (GE Healthcare) and then a Superdex 200 10/300 GL size-exclusion column ...
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bioRxiv - Cell Biology 2019Quote: ... 250 mM imidazole and 5 mM MgCl2 and loaded to a StrepTrap HP column (GE Healthcare) equilibrated with buffer containing 50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein mixture was then purified through two tandem 5-ml MBPTrap HP columns (GE Healthcare). After loading ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Microbiology 2020Quote: ... The medium was passed over a 5 ml HiTrap Chelating column charged with nickel (GE healthcare), washed with 30 ml of buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 and mtEFG2 were isolated via affinity purification on HisTrap FF 5 ml columns (GE Healthcare) and standard buffers as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... the eluted sample was transferred to a 5 ml StrepTrap column (GE Healthcare, Cat. No. 28907547), which was pre-equilibrated with elution buffer 2 (600 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM TCEP and loaded to 5 ml HiTrap SP HP (GE Healthcare, 17-1152-01). After extensive washing ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap HP 5 ml Ni-based column (Cytiva, former GE Healthcare) using ÄKTA Explorer System (Cytiva ...
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bioRxiv - Biophysics 2022Quote: ... All different DinB variants were purified using a 5 ml Ni2+-Histrap HP column (GE Healthcare) column equilibrated with buffer A and subsequently eluted with buffer B (buffer A supplemented with 1 M imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto GSTrap FF 5 mL (GE Healthcare, USA) using a buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The complexes were step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... filtered and injected in a Superdex 200 Increase 5/150 GL column (GE Healthcare Life Sciences) using a 50 μL loop ...
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bioRxiv - Microbiology 2022Quote: ... and applied onto a HisTrap HP 5 ml affinity chromatography column (GE Healthcare, Chicago, United States) equilibrated with binding buffer (20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatant was loaded onto a 5 mL Ni2+-affinity column (HiTrap chelating column, GE healthcare), preequilibrated with buffer A ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant of the cell lysate was loaded onto a HisTrap column (5 mL-GE Healthcare) and the bound protein was eluted with an imidazole gradient (0-1 M) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were initially applied to a HisTrap FF crude 5 mL (GE Healthcare UK Ltd.), which were pre-equilibrated with the HisTrap binding buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
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bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto a HisTrap FF 5 mL (GE Healthcare, Marlborough, MA, USA) preequilibrated with 50 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... then poured onto loaded onto a pre-equilibrated HiTrap DEAE FF 5 ml column (GE Healthcare) into Buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions were pooled and loaded onto a 5 ml anionic exchanger (Q-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied at 5 ml/min over 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... the clarified lysate was added to a 5 ml HisTrap High Performance column (GE Healthcare/Cytiva), washed with 5 column volumes of lysis buffer supplanted with 6 M urea ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions were pooled and loaded onto a 5 ml anionic exchanger (Q- column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2023Quote: ... The cell extract was applied onto a HisTrap™ FF crude column (5 ml; GE Healthcare) pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... then supernatant was loaded onto a 5 mL Ni2+ affinity column (HisTrap HP, GE Life Sciences) pre-equilibrated with resuspension buffer ...
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bioRxiv - Biophysics 2023Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was then circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that was inoculated with 100mM CoCl2 for ten minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... The PEO solutions are additionally filtered using a 5 µm-pore size membrane from GE Healthcare life sciences ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a 5 ml HiTrap Q HP column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were separated from unincorporated fluorophores on desalting columns (NAP-5 or PD-10, GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Gels were UV irradiated for 5 minutes and imaged using an Amersham Imager 600 (GE Life Sciences). Gels were then equilibrated in Tris-glycine transfer buffer for 5 minutes and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The first purification step was affinity chromatography with a 5 mL HisTrap™ FF column (GE Healthcare) precharged with Ni2+ and equilibrated in buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... elution from the HisPur™ Cobalt Resin was applied to a 5 ml streptactin column (GE Healthcare) and washed with HBS ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant Mtb-MenD protein was purified by IMAC with 5 mL HisTrap HP columns (GE Healthcare) using an imidazole gradient of 20–500 mM over 90 mL ...