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Citations for GE Life Sciences :
1101 - 1150 of 1791 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and injected on to a Superose 6 10/300 Increase GL size-exclusion chromatography column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Ion exchange chromatography was performed using either 6 ml RESOURCE S or RESOURCE Q column (GE Healthcare) pre-equilibrated with 5% of buffer B (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Then the mixture was subjected to gel filtration chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... 250 mM imidazole) and subsequently by gel filtration using a Superose 6 16/60 column (GE Healthcare) equilibrated with 50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were purified by Ni-NTA IMAC using Ni-Sepharose 6 Fast Flow resin (GE Healthcare). The wash buffer consisted of 50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The purified protein was run over a 10/300 Superose 6 size-exclusion column (GE Life Sciences). Fractions containing the trimeric HPIV3 F proteins were pooled and concentrated by centrifugation in an Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare, Cat# 17-5172-01). Proteins were then concentrated and the concentrations were determined using BCA protein concentration kit (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... A final anion exchange purification step was performed using a Resource Q 6 ml column (GE Healthcare), using 50 mM Tris HCl pH 8 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The pooled eluate containing TRiC was further purified through size-exclusion chromatography (Superose 6 increase, GE Healthcare) and then concentrated with a Millipore Ultrafree centrifugal filter device (100-kDa cutoff).
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was resolved by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were concentrated to 100 µL and purified by SEC (Superose 6 30/100, GE Healthcare) at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... and purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with SEC buffer containing 50 mM HEPES (pH 6.5 with KOH) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was loaded onto a Superose-6 Increase 10/300GL size exclusion column (GE Healthcare/Cytiva) equilibrated with 40 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then injected over a gel filtration column (Superose 6 10/30, GE Life Sciences) equilibrated with 20mM Tris pH 8.0 and 150mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated sample was then purified by gel filtration on a Superose-6 Increase column (GE Healthcare) equilibrated with the same buffer used for the buffer exchange ...
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bioRxiv - Biochemistry 2023Quote: ... Excess of polysulfide or DTT was removed with micro Bio-Spin 6 columns (GE Healthcare, Munich, Germany) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... and then loaded onto a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The eluent was further purified by SEC using a Superose 6 increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The clarified supernatant was applied to a Ni2+-charged IMAC column with chromatography carried out on an AKTATM purification system (GE Healthcare, Chicago, IL). The loaded column was washed with additional IMAC binding buffer and then elution carried out using an elution buffer (0.5 M imidazole ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated antibodies at room temperature for 1 hr before exposure on photographic film (28906844, Amersham, UK) following incubation with ECL for 1 minute (RPN2232, GE Healthcare, IL, USA) as per manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was adjusted to 35mM imidazole and loaded onto a 5ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) equilibrated in 20mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and purified using immobilized metal affinity chromatography via a HisTrapTM HP 5 mL (GE Healthcare, Chicago, IL, USA) and an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and then applied to an initial size-exclusion chromatography (SEC) step using HiLoad 16/60 Superdex 200 (GE Healthcare, Chicago IL) in SEC200 buffer (50 mM Tris-HCl ...
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bioRxiv - Physiology 2020Quote: Distinct bands of correct size were cut from 1.5% agarose gel and isolated using illustra GFX PCR and Gel Band Purification Kit (GE Healthcare, Chicago, IL, USA), following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA). The HisTrap HP column was washed with wash buffer (25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL GSTrap HP (GE Healthcare, Chicago, IL, USA). The GSTrap HP column was washed with wash buffer (25 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... containing 10 mM Tris pH 8.0 and 20 mM NaCl and purified using a SEC HiLoad 26/600 Superdex 75 column (GE Healthcare, Chicago, IL, USA) in 25 mM sodium phosphate pH 6.4 with 100 mM NaCl for wt-PD-1 and PD-1 mutants or in PBS pH 7.4 for PD-L1 and PD-L1-Long.
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 2 mL in a 30 kDa MWCO spin concentrator and applied onto a HiLoad 16/600 Superdex 200 pg size exclusion chromatography (SEC) column (GE healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The self-assembled crude octamers were purified by size-exclusion chromatography on a Superdex-200 10/300 GL column attached to an AKTA FPLC (GE Healthcare, Chicago, IL). Pure fractions were identified by 15% SDS-PAGE after coomassie staining ...
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bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.
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bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 5 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed by agarose gel electrophoresis with ethidium bromide staining and purified using the Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, Chicago, IL). Reactions (10 μL ...
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bioRxiv - Microbiology 2020Quote: ... through three in-line HEPA vent filters (0.3-µm glass-fiber to 0.2-µm PTFE to 0.1-µm PTFE; Whatman, GE Healthcare Life Sciences, Chicago, IL, USA) and stored at 4°C until use ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed using 0.1% PBSt and secondary antibody incubated 1h at room-temperature using Dako Polyconal Goat Anti-Rabbit or Anti-Mouse Immunoglobulins/HRP (Aglient Technologies, Santa Clara, CA, USA) washed using 0.1% PBSt and visualized using ECL or ECL Select (GE Healthcare, Chicago, IL, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2020Quote: ... Protein separation was carried out by SDS-PAGE in 10% bis-acrylamide gels and transferred to nitrocellulose membranes (Amersham Protran; pore size, 0.45 μm; GE Healthcare, Chicago, IL, USA). The membranes were blocked with 5% fat free milk in Tris-buffered saline (TBS ...
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bioRxiv - Bioengineering 2022Quote: T1-weighted axial images of the non-dominant arm were obtained using a 3T MRI scanner (Discovery MR750w, GE Healthcare, Chicago, IL, USA) and a receiver 16-channel flex coil ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or medium alone for 30 min at room temperature and added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Synthetic Biology 2020Quote: ... for 1 minute in the dark and either exposed to an X-ray film or scanned in an Amersham Imager 600 (GE Healthcare, IL, USA). Protease accessibility assays were performed as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... the gel was visualized with the Typhoon FLA 9500 laser scanner (FAM filter, 50 µm pixel and 450 V unless otherwise noted, GE Healthcare, Chicago, IL).
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bioRxiv - Neuroscience 2019Quote: ... each volunteer received a high-resolution structural 3D T1-weighted magnetic resonance scan acquired on a General Electric MR750 3T scanner (MR750; GE Healthcare, Chicago, IL).
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bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 10 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...