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Citations for GE Life Sciences :
1101 - 1150 of 1372 citations for CD122 IL 2RB Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... RNA was cleaned up using solid phase reversible immobilization (SPRI) beads (Sera Mag™, GE Healthcare Life Sciences, Chicago, IL, USA) in a homemade bead binding buffer containing 22% polyethylene glycol (PEG) ...
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bioRxiv - Bioengineering 2023Quote: ... Canada) through 0.6 and subsequently 0.08 µm polycarbonate membranes (10417206 and 110604, Nuclepore Track-Etch Membrane; Whatman, GE Healthcare, Chicago, IL) sandwiched by polyester drain discs (230600 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were treated with EZ-ECL (Biological industries, Beit-Ha’emek, Israel) and visualized using ImageQuant LAS 4000 (GE Healthcare, IL, USA). Blot intensities were quantified using the FIJI ImageJ software86 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of each sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), and the dry membrane was incubated in NRP solution (0.01 g/ml KMnO4 in ddH2O ...
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bioRxiv - Neuroscience 2023Quote: ... The course of the radial nerve just above the elbow was visualized using ultrasound (LOGIQ e, GE Healthcare, Chicago, IL, USA). A high-impedance tungsten recording electrode was inserted percutaneously and with ultrasound guidance it was inserted into the nerve ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by the appropriate horseradish peroxidase–conjugated anti-mouse (NA931V) or anti-rabbit (NA934V) secondary antibodies (GE healthcare, Chicago, IL, USA) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (AmershamTM Protran® 0.45 µm, GE Healthcare Chicago, IL, USA). Membranes were blocked for 1 hour with 5% BSA in TTBS (15 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: Quantitative analysis of the sagittal sections was performed using the automated microscope IN Cell Analyzer 6000 (GE Healthcare, Chicago, IL, USA). Four microscope slides were loaded into the system ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Biophysics 2019Quote: ... The protein bands were subsequently imaged using an Amersham Imager 600 instrument and its accompanying software (v0.9.8) (GE Healthcare, Chicago, IL, USA) with a 1 s exposure time ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... The Nitrocellulose membrane was rinsed in phosphate buffered saline and placed in a phosphorimage cassette and a film (GE Healthcare, Chicago, IL) exposed for 24 hours ...
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bioRxiv - Microbiology 2019Quote: ... Affinity-purified proteins were polished by size-exclusion chromatography (SEC) on Superdex 75 or Superdex 200 columns (GE Healthcare, Chicago, IL, USA) equilibrated with buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... iPSC-moDC and MDM were washed with T cell expansion medium (RPMI supplemented with 10% HyClone FBS (GE Healthcare, Chicago, IL, USA), 1000 U/mL Penicillin/streptomycin ...
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bioRxiv - Biophysics 2021Quote: ... The column eluate containing cyt c was further purified through a gel filtration Superdex 75 16/600 column (GE Healthcare, Chicago, IL) phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Bioengineering 2020Quote: ... Small- (Superose 6 10/300 GL) or large-scale (Superose 6 pre-packed XK 26/70) SEC columns (GE Healthcare, Chicago, IL) were run with 500 µL or 15 mL of supernatant ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting sample was loaded onto a size exclusion column (HiPrep 16/60 Sephacryl S400 High Resolution, GE Healthcare, Chicago, IL, USA) equilibrated with filtered (0.2 μm ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare, Chicago, IL). Fractions were collected and stored at 4°C and the presence of GSTU7-His protein was confirmed by analyzing the collected fractions by SDS-PAGE.
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bioRxiv - Plant Biology 2020Quote: ... two bead-based clean-up steps were carried out using SPRI beads (Magnetic SpeedBeads™, GE Healthcare No.:65152105050250, Chicago, IL, USA). The first PCR product was cleaned using a ratio of 1:0.9 and resuspended in 17 μL of ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... and immediately processed for isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation using Ficoll-Paque Premium 1.077 g/mL (GE Healthcare, IL; 17544202) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 10 μg of total protein was separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane with either 0.4 μm pores (GE Healthcare, Chicago, IL) or 0.2 μm pores (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were prepared from healthy individuals by centrifugation using Ficoll-Paque™ PLUS (GE Healthcare, Chicago, IL, USA). CD14+ cells were isolated by positive selection using anti-CD14 conjugated microbeads (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... The relative band intensities of the small RNA products were quantified by a Typhoon FLA 7000 image analyzer (GE Healthcare, Chicago, IL). The dsRNA-cleaving activity was calculated as the relative band intensity corresponding to the small RNAs in comparison with the total intensity of all bands in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Microbiology 2021Quote: ... Concentrated protein was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare, Chicago, IL, USA) column containing 0.003% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The FITC-TB_KKG6K bound to specific phosphoinositides was detected fluorometrically with a Typhoon FLA 9500 biomolecular imager (GE Healthcare, Chicago, IL, USA) equipped with a 473 nm laser and filters for excitation/emission wavelengths of 494/520 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... or normal rabbit serum (Abclon, Seoul, Republic of Korea) was cross-linked with Protein G Mag Sepharose beads (GE Healthcare, IL, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Reconstituted vesicles were subjected to 4 freeze-thaw cycles and were then extruded with an Avanti Mini Extruder using a 0.4 µm-filter (GE Healthcare, Chicago, IL) 15 times ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated either using RBC lysis or using density centrifugation with Ficoll-Paque PLUS (GE Healthcare, IL). RBC lysis was performed with a 45-minute incubation with Dextran T500 (Pharmacosmos ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... after which it was passed through a 0.45-μm filter and loaded onto a pre-equilibrated 5-mL StrepTrap HP column (GE Healthcare, Chicago, IL) with bound SBP-FKBP12.6 fusion protein ...
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bioRxiv - Biophysics 2020Quote: The reconstituted liposomes were subjected to four freeze-thaw cycles followed by extrusion 15 times using an Avanti Mini Extruder fitted with a 0.4 μm-filter (GE Healthcare, Chicago, IL). For each assay ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA) as above.
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of each mucus sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), left to dry out ...
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were eluted and fractionated by size exclusion chromatography (SEC) using a Superdex Peptide 3.2/300 column (GE Healthcare, Chicago, IL, USA) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Bands were detected using a chemiluminescent method with Chemi Lumi One Super (Nacalai Tesque) in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL).
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... and filtering through a 0.45-μm syringe filter followed by a 0.2-μm syringe filter (Whatman GE Healthcare Life Sciences, Chicago, IL). Sterile soluble mucin was then added to the fermentation buffer at a final concentration of 0.5%.
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bioRxiv - Physiology 2023Quote: ... Bands were detected using a chemiluminescent method with Chemi-Lumi One Super (Nacalai Tesque) in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL). The primary antibody was a rabbit polyclonal anti-SLC26A4 antibody (PA5-115911 ...
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bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...