Labshake search
Citations for GE Life Sciences :
1001 - 1050 of 1336 citations for CD122 IL 2RB Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
-
bioRxiv - Microbiology 2020Quote: ... 200 ml of dense culture was filtered onto a 0.22 μm polyethersulfone Whatman Nuclepore filter (GE Healthcare, Chicago, IL, USA), flash frozen with liquid nitrogen and stored at −80 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... For each IP 100µL chromatin was pre-cleared with BSA blocked protein-G sepharose beads (GE Healthcare, Chicago, IL, USA) and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Exposure of the resultant protein bands was performed with an ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare; Chicago, IL).
-
bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and applied to an SEC step using HiLoad 16/60 Superdex 75 (GE Healthcare, Chicago IL) in SEC75 buffer (50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated with peroxidase-labeled donkey anti-rabbit IgG (#NA9340-1ML, Ge Healthcare Life Sciences, Chicago, IL, USA) with 5% nonfat dry milk for one hr ...
-
bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
-
bioRxiv - Biochemistry 2020Quote: ... and 500 μL (4 μg BSA) was injected into an AKTA™ PureL system (GE Healthcare Life Sciences, Chicago IL), and run over a Superdex 200 GL (10/300 ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
-
bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2021Quote: ... The dried seeds were placed on two layers of glass microfiber filters (Whatman GF/D, GE Healthcare, Chicago, IL, USA) moistened with distilled water in a Petri dish in the dark at 23 °C for a week (conditioning) ...
-
bioRxiv - Microbiology 2020Quote: ... and the resulting signal was developed into an image using the Typhoon FLA 9500 (GE Healthcare, Chicago, IL, United States). Densitometry measurements were determined with Image Lab 6.0.1 ...
-
bioRxiv - Microbiology 2021Quote: ... Z-stack images were acquired on a DeltaVision Elite High-Resolution imaging system (GE Healthcare Life Sciences, Chicago, IL, USA) using a step size of 0.2 μm with a 60×/l.42 oil objective ...
-
bioRxiv - Immunology 2021Quote: ... the protein bands on SDS-PAGE gels were scanned and quantified by laser densitometry (Personal Densitometer SI; GE Healthcare, IL). The densities of the Hoc ...
-
bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The gels were dried and exposed for 14 days on a Storage Phosphor Screen (GE Healthcare Biosciences, Chicago, IL, USA) and imaged with a Typhoon FLA 7000 (GE Healthcare Biosciences) ...
-
bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... Membranes were developed using ECL and images were obtained with ImageQuant LAS 4000 camera system (GE Healthcare, Chicago, IL, USA). Band intensities were quantified using ImageJ software (NIH).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Biosensors selected for further study were purified using a His6 sequence using an ÄKTA Start FPLC (GE Healthcare, Chicago, IL) as previously described (Shivange ...
-
bioRxiv - Microbiology 2020Quote: ... and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL)
-
bioRxiv - Immunology 2020Quote: ... mononuclear cells were isolated from buffy coats blood using Ficoll-Paque™ PLUS (γ=1.007, GE Healthcare, Chicago, IL, USA). Next ...
-
bioRxiv - Cancer Biology 2022Quote: MSLN complex with VH 3C9 was prepared by injecting the protein mixture to Superdex 75 column (GE Healthcare, Chicago IL), and concentrated to 12 mg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
-
bioRxiv - Microbiology 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Microbiology 2023Quote: ... the cell supernatant was collected and soluble IgG was purified using affinity chromatography (GammaBind Plus Sepharose, GE HealthCare, Chicago, IL). Purified IgG was buffer-exchanged in PBS ...
-
bioRxiv - Neuroscience 2022Quote: Biosensors selected for further study were purified via the His6 sequence using an ÄKTA Start FPLC (GE Healthcare, Chicago, IL) as previously described (Shivange et al. ...
-
bioRxiv - Physiology 2022Quote: ... M-mode parasternal long axis view was applied using 11.5 MHz transducer (GE 10S-RS, GE Healthcare, Chicago, IL, USA), connected to an echocardiographic imaging unit (Vivid S5 ...
-
bioRxiv - Neuroscience 2023Quote: ... Heart rate (pulse oximeter clipped to the distal phalanx) and respiration (mid-thoracic strain gauge; both GE Healthcare, Chicago, IL) were acquired at 50 Hz during each TMS-fMRI run.
-
bioRxiv - Molecular Biology 2023Quote: ... passed through a 0.45 µM filter and loaded onto a pre-equilibrated HisTrap HP 5ml column (GE Healthcare, IL, US). The column was washed with 50 ml lysis buffer and eluted with buffer containing 200 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Neuroscience 2023Quote: ... brains were taken out quickly and rinsed with phosphate buffered saline (PBS) (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 1% fresh protease inhibitor cocktail (EMD Millipore ...
-
bioRxiv - Biophysics 2023Quote: ... Fractions containing Aβ42 were lyophilised and further purified using a Superdex 75 HR 26/60 column (GE Healthcare, Chicago, IL), and eluates were analyzed using SDS−polyacrylamide gel electrophoresis for the presence of the desired protein product ...
-
bioRxiv - Plant Biology 2023Quote: ... The DNA bands were visualized by fluorescence imaging using a Typhoon Trio Variable Mode Imager (GE Healthcare, Chicago, IL, USA). Analyses of the binding to promoter mutants MA ...
-
bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
-
bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
-
bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
-
bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
-
bioRxiv - Plant Biology 2021Quote: ... 0.5 ml aliquots of xylem sap were filtered through Sephadex columns (PD SpinTrap® G-25, GE Healthcare, Chicago, Il, USA) using Tris-buffer (50 mM Tris HCl (Roth) ...
-
bioRxiv - Cancer Biology 2021Quote: ... filtered using a 0.45 µm Puradisc 25 mm polyethersulfone Syringe Filter (6780-2504, GE Healthcare Life Sciences, Whatman™, Chicago, IL) to remove cellular debris ...
-
bioRxiv - Biochemistry 2021Quote: ... All labeled and unlabeled G-actins were further purified by size-exclusion chromatography on Sephacryl S200-HR (GE Healthcare, Chicago, IL), stored on ice in G-buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... non-α-gal allergic donor (α-gal sIgE <0.10) were isolated using a Ficoll–Paque gradient (GE Healthcare, Chicago, IL, USA). Endogenous IgE was stripped from basophils within the PBMC fraction by incubating the cells with cold lactic acid buffer (13.4 mM lactic acid ...
-
bioRxiv - Cell Biology 2022Quote: ... GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare, IL, USA). The pac sequence was amplified from pBApo-EF1α Pur and cloned into pBApo-EF1α Neo ...
-
bioRxiv - Genetics 2019Quote: ... Following washing with TBST membranes were incubated with ECL horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Chicago, IL, USA) diluted 1:5000 in blocking buffer for one hour at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... The GST and GST fusion protein extracts were mixed with glutathione Sepharose 4B beads (71024800-GE; GE Healthcare, Chicago, IL, USA) overnight at 4 °C and the mixtures were then washed three times with ice-cold PBS ...