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Citations for GE Life Sciences :
1051 - 1100 of 1963 citations for Diethyl 2 5 diaminoterephthalate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Immunology 2022Quote: ... 5% glucose) and the proteins purified by affinity chromatography using a HiTrap Heparin High-Performance column (GE Healthcare) and gradient elution (0-100% NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Biochemistry 2020Quote: ... and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and LC8 was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... The EcA2 used for these experiments were previously desalted in a 5-mL HiTrap Desalting (GE Healthcare, USA) and eluted in 10 mM sodium phosphate at pH 7.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing Pol I were then loaded onto a 5-mM HiTrap Heparin HP column (GE Life Sciences) equilibrated in Heparin BufferA(50mMTris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence was detected after 5 min of incubation with ECL™ Select Western Blotting Detection Reagent (GE Healthcare) in a ChemiDoc chemiluminescence detector (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Cell Biology 2024Quote: ... We then applied the supernatant onto two tandem 5 mL HiTrap Q HP anion exchange columns (GE Healthcare) pre-equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... blots were incubated at room temperature for 5 min in the dark with ECL detection reagent (GE Healthcare), made up in accordance with manufacturers’ instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The Spt4/5 heterodimer was further purified by size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare) through isocratic elution in low salt buffer ...
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bioRxiv - Biophysics 2023Quote: ... centrifuged and applied to a cation exchange chromatography column (5 mL HiTrap SP HP, GE Healthcare Life Science) equilibrated with 25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Biophysics 2022Quote: ... with a cut-off of 5 kDa and injected into HiLoad Superdex 200 prep grade chromatography (GE Healthcare) equilibrated with buffer C (20 mM Hepes-NaOH ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then transferred to a 5 mL tube containing 300 μL CaM-Sepharose resin (GE Healthcare) equilibrated in 50 mM HEPES (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... RRM2 (>5 mg mL-1) was injected onto a 24 mL Superdex 75 size exclusion column (GE Healthcare) at a flow rate of 1 mL min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess HaloTag ligand was then removed by purification on desalting NAP-5 or PD-10 columns (GE Healthcare). To generate the HRP-his6Halo-hPKR-N conjugate ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5% glycerol and further enriched with ion exchange Mono S HR 16/10 (SOURCETM 30Q GE Healthcare) to 95% purity ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequently loaded onto a HiTrap SP (CEH-37)/Q (POT proteins) HP 5 mL column (GE Healthcare). An appropriate salt gradient was applied using 20 mM HEPES pH 7.5 buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.22 μm filter and applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 CV of buffer Q ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was diluted 10-fold and applied to a 5 mL HiTrap Q HP column (GE Healthcare) for ion exchange chromatography using a 50 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: ... 2) the reaction mix was purified using anion exchange column (HiTrap QFF 1 mL; GE Healthcare); 3 ...
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bioRxiv - Immunology 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) using a PD-10 desalting column (GE Healthcare, USA). Light chain of human enterokinase (10 μg/ml ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysates were loaded first with 2 ml of DEAE Sepharose fast flow (GE Healthcare) and the elutes obtained by a gradient of 100-500 mM NaCl in 50 mM NaH2PO4 ...
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bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
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bioRxiv - Cancer Biology 2021Quote: ... and after 1 and 2 days of co-culture on an INCell Analyzer 2200 (GE Healthcare), with a large field-of-view sCMOS camera (resolution 2048×2048 pixels ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Neuroscience 2020Quote: The two animals (Monkey 1 and Monkey 2) were scanned on a Discovery MI (GE Healthcare) PET/CT scanner ...