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Citations for GE Life Sciences :
1301 - 1350 of 1963 citations for Diethyl 2 5 diaminoterephthalate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were cultured at 37°C in 5% humidified CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma or GE Healthcare), supplemented with 4 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Immunology 2020Quote: ... tissue was minced with scissors into 5 mm pieces and digested three times with 0.25% trypsin (GE Healthcare Life Sciences) for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... the clarified material was filtered with a 0.22 µm membrane and then applied a HisTrap HP 5 ml (GE Healthcare) and eluted with buffer B (30 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and loaded onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated in binding buffer ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5) for subsequent anion exchange chromatography on a 5 ml Mono-Q column (GE Healthcare Europe GmbH, Freiburg, Germany). Following an elution gradient with high-salt buffer (20mM TRIS ...
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bioRxiv - Cell Biology 2022Quote: ... Data were collected following fractionation with an in-line size-exclusion chromatography column (Superdex 200 5/150 Increase, GE Healthcare,) pre-equilibrated in TBS pH 8.5 ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biochemistry 2022Quote: ... Histones were then further purified by cation exchange on a 5 ml HiTrap SP HP column (GE Healthcare Life Sciences), pre-equilibrated with HGFB150 (full-length histones and tailless H2B) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM dithiothreitol (DTT), and concentrated using Vivaspin 5 kDa (HipBLp, HipSLp) and 10 kDa (HipTLp) cutoff columns (GE Healthcare). Protein concentration was quantified using the Bradford assay according to the manufacturer’s instructions (BioShop) ...
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bioRxiv - Neuroscience 2022Quote: ... The unbound fluorophores were removed by gel filtration on a NAP-5 column (Sephadex-25 medium, GE Healthcare, Munich, Germany). The final antibody concentration and degree of labelling was analyzed on a spectrophotometer (NanoDrop 2000 UV-Vis ...
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bioRxiv - Biochemistry 2022Quote: ... monomeric C9 samples were passed through a Superdex 200 Increase 5/150 GL size-exclusion column (GE Healthcare Life Sciences) in 10 mM HEPESNaOH ...
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Biophysics 2024Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using the Chemidoc XRS imaging system (Bio-Rad)..
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged at 40,000 rcf for 1 h and the supernatant was filtered through a 0.45 μM syringe filter (Chromafil GF/PET 45, MachereyNagel) and passed through a HiTrap SP HP (5 mL, GE Healthcare) column connected to an NGC FPLC system (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was diluted 5-fold to contain 100 mM NaCl and purified over SP-Sepharose (HiPrep SPFF, GE Healthcare) using a linear gradient of buffer containing 2 M NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 and equilibrated with wash buffer (50 mM K-HEPES pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biophysics 2023Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using CL-Xposure films (Cat.no ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Biochemistry 2024Quote: The Ni-NTA elution was immediately loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare, Chicago, IL, USA) that was equilibrated with 20 mM HEPES (pH 7.5) ...