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Citations for GE Life Sciences :
1051 - 1100 of 1549 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant of the lysate was filtered through a 0.22 µm membrane filter and loaded onto a Ni-NTA column (HiTrap, 5 ml, GE Healthcare) using a low imidazole buffer (Buffer C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA IMAC column (GE Healthcare) equilibrated with buffer A (50 mM KH2PO4 pH 7 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered using a 0.2 μl filter and flown over a 5 ml StrepTactin-Sepharose column (GE Healthcare) equilibrated with buffer A (50 mM MES-NaOH ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA Immobilised Metal Affinity Chromatography (IMAC) column (GE Healthcare) equilibrated with buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 15,000xg for 5 min and supernatants were incubated with 50 μL of Streptavidin Sepharose High Performance beads (GE Healthcare) for 1 h rotating ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL imidazol gradient) or by size exclusion chromatography (SEC) using a Superdex® 75 10/300 GL (GE Healthcare) with a flow rate of 0.5 mL/min and dialysis buffer as running buffer ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was filtered using a 0.2 μM filter and loaded onto a 5 ml HisTrap HP Ni-affinity column (GE Healthcare). Protein was eluted in a gradient of 10 to 300 mM imidazole over five column volumes ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing MBP-AIM2 were pooled and purified by ion-exchange chromatography using 5 mL HiTrap-SP column (GE Healthcare) equilibrated in 20 mM HEPES ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... All immunoblots were visualized with ECL Plus Detection Reagent and scanned with a Typhoon 5 Variable Mode Imager (GE Healthcare). Results are expressed as means ± SEM and expressed relative to the “no ligand” condition.
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bioRxiv - Neuroscience 2022Quote: ... All immunoblots were visualized with ECL Plus Detection Reagent and scanned with a Typhoon 5 Variable Mode Imager (GE Healthcare). Results are means ± SEM from 5-6 mice per genotype and expressed relative to TrkAWT mice.
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bioRxiv - Immunology 2022Quote: ... The lysed cells were centrifuged and only the supernatant with the soluble proteins was then further filtered with 0.45 µm filters and loaded onto two connecting 5 mL HisTrap IMAC FF columns (GE Healthcare) which were pre-equilibrated with 50mM phosphate buffer with 500 mM NaCl and 20 mM imidazole at pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... to remove cell debris the supernatant was filtered through a 0.45-μm pore size filter and then loaded onto a 5-ml Ni-HiTrap column (GE Healthcare) that had been pre-equilibrated in the same lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plates were incubated at 37° C and imaged after 20 h with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare) using the following settings ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation for 45 min at 48.000 xg and loaded onto a HisTrap FF 5-ml column (GE Healthcare). Eluted proteins were incubated with TEV protease at 4 °C overnight and both the His6-tag ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mM imidazole pH 7.5 was added and the solubilized membranes were loaded on a pre-equilibrated 5 ml Ni-NTA column (GE Healthcare) at 3 ml/minute ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Sample was filtered through a 0.45 μm nitrocellulose filter and loaded onto a 5 ml HisWash1-equilibrated HisTrap HP column (GE Healthcare). 2 ml fractions were collected through a linear gradient over 100ml up to 100% HisElution [50 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown at 37°C with 5% CO2 in a humidified atmosphere in DMEM/HIGH glucose (Cat# SH30022.01, GE Healthcare) for SK-N-SH ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... Precipitated DNA was pelleted by centrifugation and soluble lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 7 column volumes of loading buffer before being eluted with a gradient of imidazole (20–500 mM ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was filtered and the model degrons were loaded on a HiTrap SP HP 5 ml column (GE Healthcare) in 50 mM ammonium acetate pH 4.5 ...
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bioRxiv - Biochemistry 2021Quote: ... then the soluble fraction was filtered through 0.22 μm PVDF membrane before loading onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 20 column volumes of 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... Reaction mixtures (18.5 μl) were resolved on 5% Tris-Glycine native gels and imaged on a Typhoon scanner (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... were cultured at 37°C in 5% humidified CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma or GE Healthcare), supplemented with 4 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Immunology 2020Quote: ... tissue was minced with scissors into 5 mm pieces and digested three times with 0.25% trypsin (GE Healthcare Life Sciences) for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged at 40,000 rcf for 1 h and the supernatant was filtered through a 0.45 μM syringe filter (Chromafil GF/PET 45, MachereyNagel) and passed through a HiTrap SP HP (5 mL, GE Healthcare) column connected to an NGC FPLC system (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.