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Citations for GE Life Sciences :
1001 - 1050 of 1549 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: IgG was isolated from porcine sera by passing the sera over a MabSelect 5 mL HiTrap Column (GE Life Sciences), washing with two column volumes of tris-buffered saline and elution with 0.1 M glycine ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
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bioRxiv - Microbiology 2020Quote: ... The cleared membrane fraction was loaded with a flow of 0.8 mL/min on a pre-equilibrated 5 mL StrepTrap column (GE healthcare) mounted on as AktaPurifier system (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... images were obtained every 5 min for 4 h using a 20X objective (0.75 NA, PlanApo) on a DeltaVision microscope (GE Healthcare) equipped with a CoolSNAP HQCCD camera (Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Molecular Biology 2021Quote: ... radioactive signal was impressed on a FUJIFILM Storage Phosphor screen for 5 hours and acquired with Typhon Trio (GE healthcare).
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bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and the supernatant was loaded onto a 5-ml bed volume of HiTrap Heparin column (GE Healthcare, Pittsburgh, PA, USA) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the sample was concentrated to 5 ml and loaded over the HiLoad 26/600 Superdex 200 prep grade (GE Healthcare). In order to clear the impurities which had been eluted with the complex ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied onto a 5 ml MBPtrap column (GE Healthcare). The column was washed with 10 CV of lysis buffer and the protein was eluted with 5 CV of lysis buffer supplemented with 15 mM maltose ...
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bioRxiv - Immunology 2021Quote: ... the supernatant of stable HEK293S cells expressing αMβ2 headpiece was recovered and purified by immobilized ion-affinity chromatography using a 5 mL HisTrap Excel column (GE Healthcare). The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was boiled at 98 °C for 5 min, applied to SDS page gel (SuperSep, WAKO) and transferred onto a 0.2 µM nitrocellulose blotting membrane (GE Healthcare). After incubation with blocking buffer (5% milk in tris-buffered saline with 0.1% Tween20 ...
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bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
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bioRxiv - Genomics 2020Quote: ... 20 µl PCR products were subsequently immobilized to 5 µl Streptavidin Sepharose High Performance Bead (GE Healthcare, Piscataway, NJ, USA), and then were finally annealed to 1 µl sequencing primer (5 μM ...
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bioRxiv - Microbiology 2021Quote: ... The resulting lysate was centrifuged for 30 minutes at 20,000 rpm to remove cell debris and the supernatant was filtered with a 0.45 μm filter before loading onto an equilibrated 5 ml His Trap HP column (GE healthcare) connected to an ÄKTA pure 25 system ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Bioengineering 2021Quote: The yielded supernatant was passed through a 0.2 μm filter and loaded with a flow rate of 0.5 mL/min on a 5 mL HisTrap HP column (GE Healthcare) equilibrated with 12 CV binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... peak fractions were diluted to 100 mM NaCl and loaded onto a 5 ml HiTrap Q XL column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biophysics 2021Quote: ... Pooled eluate fractions were adjusted to 150 mM NaCl and purified on a 5 ml HiTrap Heparin column (GE Healthcare) using a linear gradient from 150 mM NaCl to 1 M NaCl in buffer A ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μL of Amersham ECL Rainbow Marker was loaded as a molecular weight standard (GE Life Sciences, Pittsburgh, PA). Proteins were transferred onto nitrocellulose and incubated with primary antibody ...
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The co-expressed complex of ctCyclin H and ctMAT1short was loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated in lysis buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was filtrated through a O.22μm pore filter and loaded on a 5 ml pre-packed Hitrap Q FF column (GE Healthcare) equilibrated with buffer A (50mM bis-tris-HCI pH 6.0 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was subjected to immobilized-metal affinity chromatography (IMAC) using a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB1 containing 20 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation for 30 min at 47,000 xg and the supernatant was loaded onto a 5 ml GSTrap column (GE Healthcare). The resin was washed by the lysis buffer and bound protein was eluted with elution buffer of 100 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared by centrifugation and it was applied to a 5 ml GSTrap FF column (GE Healthcare, 17513101) to purify GST-proteins ...
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bioRxiv - Cell Biology 2021Quote: ... His-Bro1ΔAPRR purified from BL21-DE3 using Ni2+-affinity chromatography (5 ml HiTrap Chelating FF column; GE Healthcare, United Kingdom). GST-Bro1V(370-709 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM TCEP) and the sample was loaded onto a 5 mL HiTrap Heparin HP affinity column (GE healthcare) pre-equilibrated with 5% buffer B (50 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of HIC-EBA was subjected to affinity chromatography using Strep-Tactin® column (5 ml cartridge, GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...