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Citations for GE Life Sciences :
951 - 1000 of 4827 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Developmental Biology 2021Quote: ... Full-length coding regions were amplified and inserted in frame into pET-28a(+) (69864-3, MilliporeSigma) and/or pGEX-4T-1 (28-9545-49, GE Healthcare) to create N-terminal ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Time-lapse imaging of one-cell embryos expressing CYB-1::mNG or mNG::CYB-3 was performed on a widefield deconvolution microscope (DeltaVision Elite; Applied Precision) equipped with a charge-coupled device camera (pco.edge 5.5 sCMOS ...
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bioRxiv - Immunology 2021Quote: ... 2-D and 3-D images were obtained and analyzed using the software program MicroView (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Microbiology 2021Quote: ... The resultant nickel column eluate was loaded onto a 5 ml SOURCE 15Q column (GE Healthcare), instead of 15S column ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was filtered (0.45 μm) applied to a 5 ml Ni-NTA column (GE Healthcare, Sweden) and eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Biophysics 2020Quote: ... and 5% glycerol at pH = 5.9 for HiTrap™ Heparin HP column (GE Healthcare Lifescience, 17040601). p53-R248Q is eluted with linear gradient of NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: CMV clone 5 cells were maintained in McCoy’s 5A media without phenol-red (GE Healthcare SH30200.01), supplemented with 10% fetal bovine serum (Gemini Bio ...
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 5 μg of PCR products were transformed into the haploid yeast strain BY4741 (GE Healthcare Dharmacon Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... 1mM DTT) and loaded onto a 5 ml Hitrap Q sepharose anion exchange column (GE Healthcare). A gradient from 0 % to 100 % high salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... the first step purification was done on a 5 mL HisTrap™ HP column (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labelled at the 5′ end with A647-maleimide or Cy5-maleimide conjugates (GE Healthcare) using the 5′ EndTag kit (Vector Labs ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleaved G5-TPR was loaded onto a 5 ml HiTrap S HP column (GE Healthcare, USA). Whereas the MAH6SSG_MBP tag is recovered in the flow-through ...
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bioRxiv - Biochemistry 2019Quote: ... and the supernatant was loaded onto a Ni2+ affinity column (HisTrap HP 5 ml, GE Healthcare). The column was washed with 5 column volumes (CV ...
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bioRxiv - Biophysics 2019Quote: ... Eluted protein was further purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to a 5 ml HiTrap HP Q column (GE Healthcare Biosciences). The flow-through ...
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bioRxiv - Biochemistry 2021Quote: ... and protein loaded onto a GSTrap FF 5 mL column (GE Life Sciences, Marlborough, MA, USA) using the NGC Chromatography system (Bio-RAD) ...
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bioRxiv - Biochemistry 2021Quote: ... The flow through was collected and loaded onto a MonoQ 5/50 GL column (GE healthcare) with buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a Ni2+-NTA column (5 ml HisTrap FF column, GE Healthcare) and the protein was eluted with an imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex were then applied onto a 5 ml HiTrap Heparin column (GE Healthcare), and after washing with 5 CV of lysis buffer the bound material was eluted with 4 CV of Heparin elution buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Peak fractions containing bS1 were directly loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated with Strep binding buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... 32P phosphorylation signals were detected using an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences) and quantified using ImageQuant TL (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was added to 5 ml of Chelating Sepharose Fast Flow resin (GE Healthcare), which had been loaded with 0.1 M NiCl2 and equilibrated with 100 ml of 50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... aSyn was buffer exchanged into 5% D2O and 95% H2O using PD10 desalting columns (GE Healthcare). CsI and NaCl were added to a final concentration of 150 mM and 1.5 M just before performing the experiments.
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bioRxiv - Plant Biology 2020Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The MBP-OsHIPP19-HMA/6xHis-SUMO-AVR-PikF complex was step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was loaded on a 5 ml HisTrap FF nickel affinity column (GE Healthcare), pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... diluted crude extract containing Nla28-DBD was loaded onto 5 ml MBPTrap HP columns (GE Healthcare) at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC ...