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Citations for GE Life Sciences :
51 - 100 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysates containing Hb were initially purified using a Zn2+ charged chelating Sepharose Fast Flow resin column (GE healthcare) because Hb surfaces are rich with His residues that can bind Zn2+ (also see (42)) ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Neuroscience 2021Quote: ... Biotinylation was quenched and free biotins were separated using a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Developmental Biology 2020Quote: ... containing 10% FBS (HyClone; GE Healthcare) and 1x penicillin/streptomycin (from 100x stock ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 10% FBS Hyclone (GE Healthcare), 100 U/mL penicillin (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were separated from supernatant by centrifugation and supernatant containing the secreted proteins was loaded onto a NiFF (GE Healthcare) column as described above.
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bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The PLPs were pelleted from the lysed cells and purified over a sizing column containing Sephacryl S1000 matrix (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The reaction was quenched and free biotins were separated using a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% fetal bovine serum (GE Healthcare), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% fetal bovine serum (GE Healthcare), 100 U/ml penicillin ...
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bioRxiv - Systems Biology 2020Quote: ... Whole blood was diluted with room temperature RPMI medium in a 1:2 ratio to facilitate cell separation for other analyses using the SepMate PBMC isolation tubes (STEMCELL) containing 16 mL Ficoll (GE Healthcare). Diluted whole blood was centrifuged at 1200 g for 20 minutes at 20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2, cells were changed with fresh media containing RMPI1640 (Thermo, 21870) supplemented with 0.2% Hyclone FBS (GE Healthcare, SH30070.03) 100 ng/mL Activin A (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated anti-mouse and anti-rabbit secondary antibodies (Cell Signaling or GE Healthcare) were used at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then incubated overnight with anti-BrdU antibody (RPN202, GE Healthcare, Buckinghamshire, UK) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTA micro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTAmicro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Capture of biotin-tagged nascent DNA was performed by incubating samples with streptavidin sepharose high performance (GE Healthcare Life Sciences) agarose beads for 16 h ...
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bioRxiv - Biophysics 2022Quote: ... The biotinylated protein was separated from excess biotin on a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in 1x PBS buffer (10 mM Na2HPO4·7H2O ...
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bioRxiv - Cancer Biology 2021Quote: ... a cell suspension containing 20,000 cells per 50uL was prepared in Biolog IF-M1 containing 0.3mM glutamine and 5% dialyzed FBS (dFBS) (Hyclone GE Life Sciences, SH30079.01) and plated into PM-M1 and PM-M2 96-well plates at 50 μL and 20,000 cells per well ...
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bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Biophysics 2020Quote: ... 3’ biotinylated RNA constructs (linked via an extended TEG spacer arm) were purchased from Integrated DNA technologies (Baulkham Hills, NSW) and immobilised on a Biotin CAPture chip (GE Healthcare) in 10 mM sodium acetate pH 4.8 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% bovine calf serum (GE Healthcare SH30072.03), 2 mM L-glutamate along with penicillin and 100 μg/ml streptomycin (Wisent 609065 ...
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bioRxiv - Biochemistry 2019Quote: ... containing an N-terminal PreScission protease (GE Healthcare) cleavable His6-tag prior to MCS 1 ...
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bioRxiv - Neuroscience 2022Quote: ... containing Cy3-conjugated streptavidin (1:1000) (GE Healthcare). To probe expression of different molecular markers in labeled neurons ...
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Immunology 2019Quote: ... containing 10% fetal bovine serum (GE Healthcare, Pittsburgh, PA); 50 ng/ml Granulocyte-macrophage colony-stimulating factor (R&D Systems ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 10% fetal calf serum (GE Healthcare, Glattbrugg, Switzerland), detached using dissociation buffer (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 10% bovine calf serum (GE Healthcare cat# SH30072.03), 2 mM L-glutamate (Wisent cat# 609065 ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10% bovine calf serum (GE Healthcare cat# SH30072.03), 2 mM L-glutamate (Wisent cat# 609065 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10% bovine growth serum (BGS; GE Life Sciences), 10 units/mL penicillin ...
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bioRxiv - Cell Biology 2023Quote: ... containing 10% bovine calf serum (GE Healthcare cat# SH30072.03), 2 mM L-glutamate (Wisent cat# 609065) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 10% bovine calf serum (GE Healthcare cat# SH30072.03), 2 mM L-glutamate (Wisent cat# 609065) ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... Fractions containing AdhE were loaded on a Superdex200 (GE Healthcare) size exclusion column ...
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bioRxiv - Neuroscience 2020Quote: ... containing a 0.2 μM cellulose acetate membrane (Whatman, GE Healthcare) as described in (Torres et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... loaded to a gravity column containing Ni-Sepharose (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 8 ...