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Citations for GE Life Sciences :
1 - 50 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Biotin CAPture Reagent provided in the Biotin CAPture Kit (GE Healthcare) was captured onto the sensor chip according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting LdCsm effector complex preparation was further purified by size exclusion chromatography (SEC) with Superdex 200 (GE Healthcare) using the chromatography buffer [20 mM Tris-HCl (pH 8.5) ...
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Molecular Biology 2019Quote: ... 40 μg of GST-FG-repeat were incubated with 50 μL Glutathione Sepharose 4B beads (GE healthcare) at 4°C for 3 h ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... 300 μl plasma from WT and KO mice after 6 months of WD feeding were analyzed by FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare)] ...
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bioRxiv - Cell Biology 2019Quote: ... 6His-Cdc11-SNAP was biotinylated with BG-Biotin and subsequently immobilized on a CAP chip (Biotin Capture Kit; GE Healthcare, Freiburg, Germany). Preparation of the chip ...
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bioRxiv - Biophysics 2019Quote: ... The mAb TS2/4-biotin was purified from an excess of NHS-LC-LC-Biotin via gel filtration (Superdex-200 10/300; GE Healthcare Life Sciences), concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Immunology 2020Quote: ... Cell culture medium containing 20 % FBS (SH30066.03, HyClone, GE Life Sciences), 10 mM HEPES (15630-080 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then resuspended in medium containing Percoll (GE Healthcare), centrifuged at 60 × g for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01 ...
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bioRxiv - Bioengineering 2019Quote: ... containing 500 mM glucose and HyClone Cell Boost 7b Supplement (GE Healthcare) were fed daily when cell numbers reached 1.4 − 2.2 × 106 cells/mL at 2% and 0.2% of daily total volume ...
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bioRxiv - Immunology 2020Quote: ... Cells were frozen down in freezing medium containing FBS (Hyclone, GE Healthcare, SH30071.03) and 10 % dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... Biotin excess was removed by SEC on a Superdex 200 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Biophysics 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... followed by the removal of biotin by desalting on PD10 columns (GE Healthcare). The desalted lysates were then applied to streptavidin agarose resin (100 µl ...
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bioRxiv - Immunology 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare). The control MHC-I (HLA-A2 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated proteins were immobilized on a CAP chip (Biotin CAPture kit, GE Healthcare). As a blocking control anti-LILRB2 antibody ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with TBST containing horseradish peroxidase-conjugated secondary antibody (1:1000; GE Healthcare, Pittsburgh, PA) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Biotin-labeled SUMO-fusion TRIM14 was coupled on the sensor chip SA (GE Healthcare). Dilution series of TBK1 or IRF-3 (1.25 ...
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Immunology 2020Quote: ... The fractions containing the antibody were pooled and elution buffer was exchanged for PBS using NAP-25 columns (GE Healthcare). Purified VHH72hFc1X7 in PBS was quantified by absorbance at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting elutes containing antibodies were applied to a size exclusion chromatography column (Superdex 200 10/300 GL; GE Healthcare) with PBS served as running buffer and a flow rate of 0.5 mL/min (SI Figure 8) ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS containing cell lysate was directly applied to a HiTrap Heparin HP column (GE Healthcare) equilibrated with Binding Buffer with 130 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated biotin and aggregated viruses were removed by NAP-5 gel filtration columns (GE Healthcare) and 0.2 μm pore size filters ...
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bioRxiv - Microbiology 2021Quote: ... Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS containing 0.05 % Tween®20 (PBS-T) and incubated with HRP-conjugated antibodies (GE healthcare) for 3 hours at RT ...
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bioRxiv - Immunology 2021Quote: ... Biotin excess was removed by SEC on either a Superdex 200 10/300 column (GE Healthcare) for the RBD probe or a HiLoad Superose 6 16/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and unincorporated biotin-17-dCTP was removed by using S-200 HR Microspin columns (GE Healthcare). The DNA was ethanol precipitated and resuspended in TE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin-labelled PCR amplicons were captured by mixing PCR products with streptavidin-sepharose beads (GE Healthcare). Vacuum Prep Tool with filter probes was used to capture sepharose beads with biotin labelled PCR amplicons ...
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bioRxiv - Biochemistry 2021Quote: ... Biotin incorporation was assessed by incubating blots with streptavidin-HRP (1:1000 v/v, GE Healthcare), and vimentin levels estimated by western blot with anti-vimentin V9 monoclonal antibody (1:1000 v/v ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labelled HCV 3’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
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bioRxiv - Immunology 2021Quote: ... All primary antibody incubations were followed by incubation with appropriated secondary HRP-conjugated antibodies (GE Healthcare or Cell Signaling) in 5% milk plus 0.1% Tween20 (Sigma P2287) ...
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bioRxiv - Microbiology 2024Quote: ... containing Glutamax (Hyclone, GE Healthcare) and supplement B27 (Hyclone ...
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bioRxiv - Molecular Biology 2019Quote: ... Unreacted biotin label was removed via SEC on a Superdex 200 Increase column (GE Healthcare Life Sciences). Biotinylated BSA-Lewis X was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Biophysics 2021Quote: ... Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare, Marlborough, MA).
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bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Systems Biology 2020Quote: ... The signal of biotin-bound HRP was visualized using Amersham ECL Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Excess biotin was removed by size-exclusion chromatography on a S200 10/300 GL column (GE Healthcare) with a distal 1 ml GSTrap 4B (GE Healthcare) ...