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Citations for GE Life Sciences :
51 - 100 of 178 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... for 5 m and visualized by using a phosphorimager (ImageQuant LAS 4000, GE Healthcare). For analysis ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... The secondary antibodies were HRP-linked antibodies against rat or mouse IgG (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... ECL Mouse IgG and ECL Rat IgG (1:5000, NA934, NA931, NA935, GE Healthcare) and immunoblots were imaged using NuGlow ECL (Alpha Diagnostics) ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... 0.150 M NaCl) using PD-10 or PD-MidiTrap G-25 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Molecular Biology 2023Quote: ... The beads were then resuspended in 6 M urea (#17-1319-01, GE Healthcare, UK), reduced with 30 nmols dithiothreitol (#D9163-25G ...
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bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse-IgG and anti-rat-IgG (all from GE Healthcare, Chicago, IL, USA). The antibodies were detected with Enhanced Chemiluminescence kit (Thermo Fischer Scientific).
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Biochemistry 2020Quote: ... 1.5 M (NH4)2SO4) and was loaded on a 50 ml Phenyl Sepharose column (GE Healthcare). After one column volume wash step the tRNAs were eluted with a linear gradient of Phe-sepharose B buffer (20 mM NaOAc ...
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bioRxiv - Molecular Biology 2020Quote: ... Agarose gels with TRFs were blotted in 0.4 M NaOH onto Hybond XL membrane (GE Healthcare). TRFs were hybridized with radioactively [Y-32P]-dATP labelled TEL C 5‘-(CCCTAAA)4-3 overnight at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... the beads were taken up in 100 µL 0.8 M urea (GE Healthcare, GE17-1319-01) in 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Microbiology 2020Quote: ... Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-coupled secondary antibodies (anti-mouse and anti-rabbit, GE Healthcare; anti-rat, Jackson Immunoresearch) in combination with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Immunology 2022Quote: ... and ECL anti-rat IgG Horseradish Peroxidase linked whole Ab from goat (GE Healthcare UK Limited) were used to detect IgG1 antibodies upon addition of TMB substrate (TMB substrate set ...
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bioRxiv - Biochemistry 2020Quote: ... 0.3 M NaCl using a Fast Protein Liquid Chromatography (FPLC) system (ÄKTA Purifier, GE Healthcare Life Sciences). Prior to loading ...
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bioRxiv - Cell Biology 2021Quote: ... a commercially available siRNA pool was used to knockdown ISG15 (#M-004235-04-005, GE Healthcare Dharmacon). In these experiments ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Neuroscience 2020Quote: ... The bound silver-gold particles were enhanced using a Silver IntenSE M kit (catalog RPN491; GE Healthcare) for 7 minutes.
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Pathology 2021Quote: ... The strip was covered with white mineral oil (DryStrip Fluid, M/s. GE Healthcare Life Sciences, Amersham, UK), and incubated horizontally at 22 °C for 18 h ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 M sodium citrate pH 7.0) and transferred to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) by capillary action overnight ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified using a 20 mL NiNTA column (Cytiva) attached to an Akta M FPLC (GE Healthcare) and with a 300 mM imidazole elution buffer ...
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.6 M NaCl before transfer under neutral conditions on Amersham™ Hybond-XL nitrocellulose membrane (GE Healthcare, Cat# RPN203S). After one round of UV auto-crosslink (120,000 μJ/cm2 ...
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bioRxiv - Biochemistry 2021Quote: ... the M-Lipxtal protein was further purified by SEC using a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... sample was desalted into Fab-storage buffer (20 mM HEPES pH 8.0, 0.1 M NaCl) using a pre-packed PD-10 column (GE Healthcare). Purified Fab30 was flash frozen stored in buffer supplemented with 10% glycerol.
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bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted samples were desalted into buffer (20 mM HEPES-Na+, pH 8.0, 0.1 M NaCl) using a pre-packed PD-10 column (GE Healthcare). Purified Fabs were flash-frozen and stored at −80 °C supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... The column was washed with 10 column volumes of Buffer A (10 mM HEPES, pH 7.3, 0.15 M NaCl, 5 mM imidazole) on an Aktapurifier (GE Healthcare) while collecting fractions ...
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bioRxiv - Molecular Biology 2023Quote: ... Free dye was removed with buffer exchange to carbonate-bicarbonate buffer (0.2 M NaHCO3 – Na2CO3, pH 8.5) using size-exclusion chromatography (PD-10 Desalting columns, GE Healthcare). Next ...
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with a linear 0.05 to 1.5 M NaCl gradient and further purified by gel filtration on a HiLoad Superdex 200 16/60 column (GE Healthcare) in 40 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... The plates were subsequently developed in 1.5 M KH2PO4 (pH 3.64 ± 0.03) and autoradiographed using a Storm 860 Phosphorimager (GE Healthcare Life Sciences). pGpp signal was quantitated using ImageJ software.74 RelQ activity was expressed as the percentage of γ-32P-ATP substrate converted into product at each timepoint.73 Michaelis-Menten assays were performed by running the reactions for 5 minutes at the indicated concentration of GDP or GTP ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... 150 mM CsI or 1.5 M CsI in H2O or D2O prior to performing experiments using PD10 desalting columns (GE Healthcare). 10 μM freshly made ThT (abcam ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA (1 – 10 μg) was separated using formaldehyde (2.2 M) gel electrophoresis and transferred to a Hybond-NX Nylon membrane (GE Healthcare) via capillary transfer ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...