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Citations for GE Life Sciences :
1 - 50 of 178 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... HepG2 and HEK293 cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, GE Healthcare) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Buffer was added to the supernatants to a final concentration of 0.01 M HEPES/0.2 M NaCl (pH 7.4) and passed through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.2 ...
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bioRxiv - Immunology 2023Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Neuroscience 2021Quote: ... A Tracerlab FX M module (GE Healthcare) was used for automated methylation ...
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bioRxiv - Cancer Biology 2022Quote: ... M-016083-00-0005 (GE Healthcare Dharmacon). siRNA non-targeting pool #2 (GE Healthcare Dharmacon ...
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bioRxiv - Microbiology 2020Quote: ... Binding analyses were performed in HBS-P buffer (0.01 M HEPES, pH 7.4, 0.15 M NaCl, 0.005% surfactant P20; GE Healthcare Life Sciences) at 37°C with a flow rate of 30 μl/min ...
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Cell Biology 2020Quote: ... anti-rat IgG-HRP (GE Healthcare, UK), anti-rat IgG-HRP (Cell signaling ...
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2021Quote: ... a hydrophobic interaction chromatography using a linear decreasing (NH4)2SO4 gradient (from 1.5 M to 0 M) (HiTrap HIC phenyl column, GE Healthcare) was applied to the mixture ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... and eluted with an elution buffer (20 mM NaK, 0.5 M NaCl, and 0.5 M imidazole) in an ÄKTA-Pure instrument (GE Healthcare, Piscataway, NJ). The buffer of the eluted proteins was replaced with a mesotrypsin buffer (100 mM tris(hydroxymethyl)aminomethane (Tris) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... using an ÄKTA pure 25 M system (GE Healthcare) and eluted against the same buffer including 500 mM imidazole over 10 column volumes ...
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bioRxiv - Cell Biology 2020Quote: ... mouse or rat IgG was purchased from GE Healthcare.
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bioRxiv - Cell Biology 2019Quote: ... HUVECs were cultured in HyClone M-199 media (GE Healthcare) with 1% antibiotics-antimicrobial (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatography was performed on an AKTApure M system (GE Healthcare) at a flow rate of 0.05 ml/min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatography was performed on an AKTApure M system (GE Healthcare) at a flow rate of 0.03 ml/min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Biochemistry 2021Quote: ... anti-rat IgG and anti-rabbit IgG antibodies (GE Healthcare). Blots were detected using ECL Prime Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-linked goat anti-rat IgG pAb (#NA935V, GE Healthcare) and HRP-linked rabbit anti-goat IgG pAb (DAKO #P0449 ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 M Na3PO4 (#28-9030-59, Ab Buffer Kit, GE Healthcare). Beads were incubated at 4°C on a rotator ON ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C using an ÄKTA Pure 25 M (GE Healthcare). The column was washed with 75 mM imidazole [in 20 mM NaH2PO4 and 0.5 M NaCl (pH 7.4)] ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
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bioRxiv - Plant Biology 2021Quote: ... The pellets were resuspended in buffer containing 7 M urea (GE Healthcare), 2 M thiourea (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... anti-rabbit-HRP (GE_NA934V) and anti-rat-HRP (GE_NA935V) (both GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 M ammonium sulfate) and fractionated on a DEAE Sephacel column (GE Healthcare). The flow-through fractions were further fractionated on a phosphocellulose 11 column ...
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bioRxiv - Immunology 2022Quote: ... and HEK293 cell lines were obtained from Royan Institute Cell Bank and cultured in Dulbecco’s Modified Eagle Medium (DMEM, GE Healthcare Life Sciences, Pittsburgh, PA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 M urea gel and transferred onto an Amersham Hybond-XL membrane (GE Healthcare) for Northern Blotting analysis ...
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bioRxiv - Neuroscience 2021Quote: ... The chip was deactivated by 1 M Ethanolamine hydrochloride-NaOH (GE Healthcare Life Sciences) at a flow rate of 10 μL/min for 420 s ...