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Citations for GE Life Sciences :
51 - 100 of 6090 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 1h at 20000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged protein was eluted with a linear gradient of buffer B (50 mM Hepes pH 7 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). The His-tagged protein was eluted with a linear gradient of buffer B (Hepes 50 mM pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
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bioRxiv - Biophysics 2021Quote: SPR measurements were recorded at either 25°C or 35°C using a Biacore T200 system (GE Healthcare). All experiments were performed using an SA Series S Sensor Chip (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Biochemistry 2021Quote: ... or by sonication and cleared lysate was obtained by centrifugation (24,446 x g; 30 min; 4°C) and filtration using a Whatman™ folded filter (GE Healthcare, now Cytiva).
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... onto a Hybond-C extra membranes (GE Healthcare). Membrane strips were blocked with 3% fatty acid-free BSA (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... at 25°C using Biacore T200 (GE Healthcare). The 6×His-tagged AVR-PikD (ligand ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was Hybond-C nitrocellulose (GE Healthcare), and transfer was performed in 25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... at 20 °C in HBS-EP+ (GE Healthcare) running buffer ...
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bioRxiv - Genomics 2023Quote: ... transferred onto Hybond C nitrocellulose membrane (GE Healthcare), probed with primary antibodies anti-RRM diluted 1:300 ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T were obtained from ATCC and cultured at 37°C and 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plates were incubated at 37° C and imaged after 20 h with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare) using the following settings ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown at 37°C with 5% CO2 in a humidified atmosphere in DMEM/HIGH glucose (Cat# SH30022.01, GE Healthcare) for SK-N-SH ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured at 37°C in 5% humidified CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma or GE Healthcare), supplemented with 4 mM L-glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... and the soluble proteins were isolated by centrifugation (80,000g, 45 min, 4°C) and purified by cation exchange chromatography (HiTrap SP, GE Healthcare) with a gradient of 0-1M NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates containing protein were concentrated to <500µL and size-exclusion was performed at 4°C using an ÄKTA Pure Protein Purification System (GE healthcare) and Superose® 6 Increase 100/300 GL column (Cytiva) ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed in PBS-T and incubated with 1 mL of Enhanced ChemiLuminescence (ECL) (Amersham) reagent for 5 minutes to allow protein visualization on an x-ray Amersham Hyperfilm ECL (GE Healthcare). Fiji-Image J Software was used for quantification of the difference in protein expression comparing with GAPDH or HSP70 expression.