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Citations for GE Life Sciences :
301 - 350 of 6090 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was bound to a 5 ml HiTrap HP column (GE Healthcare), and a linear gradient of increasing NaCl concentration was used to elute the protein fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleaved protein was loaded onto a 5 ml Heparin column (GE Healthcare). The protein was eluted by a NaCl gradient from 0.25 to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed protein was loaded on a 5 mL HiTrap Heparin column (GE Healthcare) equilibrated with buffer A to remove the MBP ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was desalted using a 5 ml HiTrap Desalting column (GE Healthcare) and sterile-filtered PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subsequently purified over a 5 ml HiTrap Heparin column (GE Healthcare) and a 1 ml HiTrap Q column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein purification was performed using a 5 ml StrepTrap HP column (GE Healthcare) followed by SEC using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Excess dye was removed by purifying protein over NAP-5 columns (GE Healthcare). Labeling was confirmed using NanoDrop 2000c (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: Protein samples were loaded onto a HiTrap TALON column (5 mL, GE Healthcare) connected to an AKTA prime system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... the protein was passed through a 5 mL HisTrap HP column (GE Healthcare), and the cleaved protein was collected in the flow through ...
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bioRxiv - Microbiology 2020Quote: ... Beads were isolated from the suspension by centrifugation (500 x g, 5 minutes) through Percoll Plus (GE Healthcare) in a 50 mL conical tube ...
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bioRxiv - Plant Biology 2021Quote: ... pastoris X-33 and the secreted proteins were purified by affinity chromatography (HiTrap Chelating HP column) (GE Healthcare). Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... time-lapse movies of high resolution were recorded at 37 °C on a DeltaVision Elite (GE Healthcare) widefield microscope using a 60×/1.42 NA oil objective and a step size of 0.25-0.35 μm (Figure 1A-C ...
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bioRxiv - Biophysics 2022Quote: SPR analysis were carried out at 25.0 °C using Biacore T100 system (GE Healthcare, Chicago, IL, USA). In all protocols ...
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bioRxiv - Biochemistry 2019Quote: Yeast were imaged at 30 °C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision Ltd.) comprising a wide-field inverted epifluorescence microscope (IX70 ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were used for silverstaining (PageSilver kit, Fermentas) or transfer to Hybond-C nitrocellulose mebranes (GE Healthcare). After overnight blocking in PBS with 5% nonfat dry milk and 0.1% Tween 20 ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was filtered (1.2 μm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom), acidified to pH ≤ 1 for sterilization using 30% hydrochloric acid ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Biophysics 2022Quote: ITC experiments were performed at 25°C by using a MicroCal VP titration calorimeter (MicroCal-GE Healthcare) under the same conditions as described previously (16) ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Molecular Biology 2020Quote: All the surface preparation experiments were performed at 25°C using a Biacore 3000 instrument (GE Healthcare). Purified Rubisco was immobilized on CM5 sensor chips using a Biacore Amine Coupling Kit according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was nutated (2h, 4°C) with 10ml glutathione-Sepharose 4B resin (GE Healthcare, Pittsburg, PA) in resuspension buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Separation was performed at 20°C using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with a flow rate of 0.5 mL min−1 and a mobile phase consisting of 20 mM Na-HEPES pH 7.8 ...
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The purified rh proSP-C BRICHOS variants were analysed by Superdex 200 GL columns (GE Healthcare, UK) using an ÄKTA system (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C overnight and then scanned using an Amersham Typhoon scanner (GE Healthcare). Individual infected cells were quantified using ImageQuant TL software (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... The binding experiments were performed at 25°C on a Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) in HBS-EP+ buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2022Quote: ... Gels were dried at 80 °C for 2 hrs and covered with phosphor imaging plate (GE Healthcare) overnight after RNA fixation in RNA fixation buffer (40% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the supernatant was applied to a 1 ml Ni-NTA HisTrap column (GE Healthcare). The protein was eluted with an imidazole gradient using an analogous gradient of HisTrap buffer B (HisTrap buffer A with 500 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Bioengineering 2022Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Biochemistry 2019Quote: ... Proteins were routinely desalted using 5 mL HiTrap™ desalting columns (GE Healthcare, USA) using desalting buffer (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 5 mL HisTrap HP column (GE Healthcare), washed with 10 CV of Nickel Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Digested protein was applied to a 5 mL HiTrap Q HP column (GE Healthcare) at 0.5 mL/min ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified with a 5-mL HiTrap SP cation-exchange column (GE Healthcare) and eluted with a salt gradient (10–1000 mM NaCl) ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed proteins were loaded onto two 5 mL HiTrap Heparin HP columns (GE Healthcare) connected in tandem ...