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Citations for GE Life Sciences :
51 - 100 of 126 citations for Guinea Pig Plasma With Sodium Citrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Umbilical cord blood mononuclear cells (UCBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare, Chicago, IL). UCBMC were frozen in 10% DMSO/FetalPlex (GeminiBio ...
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bioRxiv - Immunology 2022Quote: ... were also isolated from the fraction of EDTA blood remaining following the removal of plasma using a Ficoll-Paque Premium density gradient (GE Healthcare), and washed with R-10 media ...
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bioRxiv - Immunology 2023Quote: ... Plasma lipoproteins were separated using fast-performance liquid chromatography with two Superose 6 10/300 GL columns (GE Healthcare; Boston, MA) on a Shimadzu HPLC system (Columbia ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were treated with oxygen plasma for 2 min and immediately submerged in Plus-One Repel Silane solution (GE Life Sciences) for 3 min ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of the collected plasma was heat-inactivated (56°C for 40 min) and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: Plasma samples (200 μL) were loaded onto AKTA pure FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare). Lipoproteins were eluted with PBS and 0.25 mL fractions were collected at a flow rate of 0.4 mL/min.
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bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Pathology 2020Quote: ... Equal volumes of plasma were pooled from three mice and lipoproteins were fractionated by fast protein liquid chromatography (ÄKTA pure FPLC system, GE Healthcare, Pittsburgh, PA, USA) and triglyceride and cholesterol in fractions quantified with reagent kits (Wako Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Cell Biology 2020Quote: Plasma M- and Z-AAT was isolated by affinity chromatography using the AAT specific Alpha-1 Antitrypsin Select matrix (GE Healthcare Life Sciences, Cytiva, Sheffield, UK) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Immunology 2021Quote: ... Samples were buffer exchanged into NMR buffer (20 mM sodium phosphate pH 7.0, 50 mM NaCl, 0.02% NaN3) using PD10 desalting columns (GE Healthcare). Samples were concentrated further to a volume of ~300 μL ...
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bioRxiv - Immunology 2020Quote: ... The trimer fraction was purified by size exclusion chromatography on a Superdex 200 10/300 GL column in TBS + 0.1% DDM + 0.003% sodium deoxycholate using an ÄKTA Pure 25L HPLC instrument (GE Healthcare) at a flow rate of 0.75 ml/min.
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RBD was diluted to a concentration of 15 μg/mL with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units (RU) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Cell Biology 2024Quote: Samples were separated electrophoretically on a 12.5% sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) and transferred to Polyvinylidene Difluoride (PVDF, GE Healthcare) membranes at 60 mA per membrane for 90 min in a semi-dry transfer unit (TE77X Hoefer) ...
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Microbiology 2024Quote: ... The deacylated tRNA was ethanol precipitated and dissolved in monoQ buffer A (40 mM sodium phosphate buffer, pH 7.0) and separated on the 8-ml MonoQ column (10/100, GE Healthcare), using two-step linear gradient of buffer B (A with 1M NaCl) ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of lyophilized Aβ was resuspended in 700 µl GE buffer pH 8.5 (6 M guanidinium hydrochloride, 20 mM sodium phosphate) and loaded onto a Superdex 75 SEC column (GE Healthcare) equilibrated with aggregation buffer (20mM sodium phosphate ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2020Quote: ... cysteine variants of α-synuclein were buffer exchanged into PBS or sodium phosphate buffer by use of P10 desalting columns packed with Sephadex G25 matrix (GE Healthcare). The protein was then incubated with an excess of Alexa-488 or Alexa-546 dyes with maleimide moieties (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... media was diluted with an equal volume of Buffer A (500 mM NaCl, 20 mM sodium NaH2PO4, 20 mM imidazole) and loaded onto a 1 mL HisTrap column (GE Healthcare). The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl ...
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bioRxiv - Bioengineering 2020Quote: A solution of 25 mg/L SC-SWCNTs and 2 mg/mL C16-PEG2k-ceramide in 1% sodium cholate was added to a 1 kDa molecular weight cutoff dialysis cartridge (GE Healthcare). The solution was dialyzed against water with multiple water exchanges for a week ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Hybond ECL membrane (Amersham Biosciences, GE Healthcare). Membrane was stained with Ponceau S solution (Sigma-Aldrich).
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bioRxiv - Genomics 2024Quote: ... Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare). The membranes were incubated with primary antibodies in a blocking solution (2% BSA in PBS containing 0.1% Tween 20 (PBS-T) ...
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bioRxiv - Biochemistry 2022Quote: ... both MSL8N and MSL9N were placed in a high salt storage buffer (1 M NaCl, 200 mM Sodium Phosphate) using gravity PD-10 desalting columns (GE Healthcare). The protein was labeled with Alexa Fluor 647 NHS (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... ∼1 mg of protein was desalted into 100 mM sodium bicarbonate (NaHCO3) at pH 8.3 using a PD10 desalting column (GE Healthcare 17085101) according to manufacturer’s suggestions ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts of protein extracts were electrophoresed on 12% sodium dodecyl sulphate (SDS) polyacrylamide gel prior to transfer on Hybond ECL nitrocellulose membranes (GE Healthcare). Homogenous transfer was confirmed by Ponceau Red staining ...
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bioRxiv - Microbiology 2024Quote: ... were electrophoresed on 10% sodium dodecyl sulphate-polyacrylamide gel and the separated proteins were transferred onto the PVDF membranes (Amersham International, GE Healthcare). The membranes were blocked with Tris-buffered saline with Tween (TBST ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were resolved by stain-free sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane (GE Healthcare) using the semi-dry Trans-Blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were fractionated using 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes (GE Healthcare). Western blotting was performed according to standard protocols [24].
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bioRxiv - Developmental Biology 2024Quote: ... Proteins (20 μg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (10600023, GE Healthcare). After transfer ...