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Citations for GE Life Sciences :
1 - 50 of 126 citations for Guinea Pig Plasma With Sodium Citrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare). For non-covalent coupling of antibodies to beads ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 M sodium citrate pH 7.0) and transferred to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) by capillary action overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Plasma antibodies were purified using Protein A Sepharose resin (GE Healthcare), eluted from the resin with 0.1 M glycine at pH 2.5 and buffer exchanged into 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... 0.1% plasma and further fractionated on a discontinuous Percoll (GE Healthcare) gradient consisting of layers with densities of 1105 g/ml (85%) ...
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bioRxiv - Immunology 2023Quote: ... Polyclonal IgG was purified from plasma using Protein A resin (GE Healthcare) and digested into Fabs using immobilized papain resin as described previously46 ...
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bioRxiv - Microbiology 2021Quote: All surface plasma resonance assays were performed on a Biacore 3000 (GE Healthcare) with a running buffer of 10 mM HEPES pH 7.5 and 150 mM NaCl supplemented with 0.05% Tween 20 at 25°C ...
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bioRxiv - Immunology 2021Quote: All surface plasma resonance assays were performed on a Biacore 3000 (GE Healthcare) with a running buffer of 10 mM HEPES pH 7.5 and 150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... and TBS (for qmTQ2-citrate biosensor) using the PD-10 gel filtration column (GE Healthcare, Illinois, USA) to remove imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... plasma was passed through a size-exclusion column (PD-10 Desalting columns, GE Healthcare) and loaded onto a gelatin-sepharose 4B column (VWR Schweiz) ...
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bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... 1X sodium pyruvate (HyClone, GE Healthcare, Chicago, IL), and 1X non-essential amino acids (HyClone ...
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bioRxiv - Neuroscience 2021Quote: ... 20 mg/ml sodium dodecyl sulfate (SDS, GE Healthcare), 40 nM N-ethylmaleimide (NEM ...
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bioRxiv - Plant Biology 2020Quote: ... Excess iron ammonium citrate and lithium sulfide was removed by desalting using a NAP™ 25 column (GE Healthcare). Subsequently ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved products were further purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences). The purity of the protein was inspected using a 15% SDS PAGE (Figure S1) ...
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bioRxiv - Pathology 2021Quote: ... Excess reagents were removed by repeated diafiltration into fresh PBS buffer (140 mM sodium chloride and 12 mM sodium phosphates at pH 7.4) using VivaSpin sample concentrators (GE Healthcare, 10,000 Da MWCO), and the volume was corrected to 200 μL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NHR2 was then purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences) and analyzed using SDS-PAGE ...
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bioRxiv - Immunology 2022Quote: ... PBMCs and plasma samples were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and stored at -80°C until use.
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bioRxiv - Molecular Biology 2022Quote: ... and white blood cells from plasma were isolated with Ficoll-Paque™ PLUS medium (GE healthcare). Thereafter ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Coverslips were plasma cleaned using Carbon Coater (Agar Scientific) and treated with PlusOne Repel-Silane (GE Healthcare) for 10–15 min ...
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bioRxiv - Biochemistry 2021Quote: The plasma samples were depleted of IgG and albumin using Albumin & IgG depletion SpinTrap columns (GE healthcare) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Microbiology 2020Quote: ... Whole venous blood collected in Citrate-Phosphate Dextrose-Adenine-1 (CPDA-1,JMS) and PBMC were isolated from whole blood over Ficoll-Paque PLUS (GE Healthcare) density gradient ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of plasma was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare). PBMC were frozen in 10% DMSO/FBS and stored in liquid nitrogen until analysis ...
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bioRxiv - Systems Biology 2024Quote: Mouse plasma samples were subjected to fast-performance liquid chromatography (gel filtration on Superose 6 column (GE Healthcare). Different lipoprotein fractions were separated and evaluated based on flow-through time ...
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bioRxiv - Cancer Biology 2024Quote: ... The platelet-rich plasma fraction was passed through a filtration column of sepharose CL-2B beads (GE Healthcare) loaded into a siliconized glass column with a 10 µm nylon net filter (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Genetics 2020Quote: ... Plasma lipoprotein particles were size fractionated by Fast Protein Liquid Chromatography (FPLC) using a Superose 6 column (GE Healthcare) and the cholesterol content in each fraction was measured accordingly26.
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bioRxiv - Cell Biology 2023Quote: ... Fibronectin was isolated from human plasma using Gelatin-Sepharose 4B (Cat No. 17-0956-01, GE Healthcare, Sydney, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... without L-Glutamine and Sodium Pyruvate (GE Healthcare Life Sciences Cat. # SH30081.01); 1X GlutaMAX-I (Thermo Fisher Scientific Cat ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5% Sodium dodecyl sulfate (SDS; GE Healthcare, cat. no 17-1313-01), 8% glycerol (Alfa Aesar ...
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bioRxiv - Neuroscience 2020Quote: ... 50-250 μl of plasma were used for immunoglobulin purification via 1:1 mixture of proteinA/proteinG resin (GE Healthcare). Slurry was washed once with PBS ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2022Quote: Fibronectin was purified from outdated human plasma (American Red Cross, Rochester, NY) using gelatin-Sepharose (GE Life Sciences, now Cytiva) affinity chromatography (102) ...
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Immunology 2023Quote: IgG was purified from plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 150 mM sodium chloride (HBS) and loaded onto protein G resin (GE Healthcare) equilibrated with HBS ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Pathology 2023Quote: Peripheral blood mononuclear cell (PBMC) was isolated from whole blood collected in citrate tube by ficoll gradient method (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). 500.000 PBMC /well was inoculated in 24 well plate in 1 ml ...
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bioRxiv - Microbiology 2021Quote: ... Lithium heparin-coated tubes were used for blood collection and plasma was isolated using Ficoll-Hypaque (GE Healthcare; 17-1440-03) in accordance with manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... blood samples were collected during hospitalization and follow-up visits and separated into plasma and peripheral blood mononuclear cells (PBMCs) by Ficoll-Hypaque gradient (GE Healthcare) centrifugation ...
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bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
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bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...