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Citations for GE Life Sciences :
901 - 950 of 5333 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated with GFP nanobody-conjugated affinity resin (CNBr-activated Sepharose 4B resin from GE Healthcare) by rotating at 4°C for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Microbiology 2021Quote: ... Enzymes were isolated from the cleared lysates via nickel affinity chromatography (IMAC) using a HisTrap column (GE Healthcare) and size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant applied to Ni-NTA agarose followed by glutathione-agarose affinity column (GSTrap HP, GE Healthcare). AP-2-containing fractions were pooled ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was applied to a 5ml IMAC (Immobilized Metal-Ion-Affinity Chromatography) HiTrap Chelating column (GE Healthcare) preloaded with 100 mM zinc sulphate and equilibrated with low IMAC1 buffer (50 mM Na-Phosphate buffer ...
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bioRxiv - Biophysics 2019Quote: ... The solubilized fraction was subjected to affinity purification by 1D4 antibody conjugated to CNBr-activated agarose (GE Healthcare). The resin was extensively washed and the protein eluted with wash buffer supplemented with 0.2 mg/ml 1D4 peptide ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was subjected to an affinity purification using a chelating HiTrap FF crude column (GE Healthcare) charged with Ni2+ ions on an AKTA-HPLC purifier (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: The affinity of nanobodies was measured by surface plasmon resonance (Biacore 3000, GE Healthcare Bio-Sciences AB, Sweden). Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5 ...
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bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions and as previously described (83) ...
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bioRxiv - Molecular Biology 2023Quote: Surface plasmon resonance (SPR) affinity and kinetic analysis was carried out using the Biacore T200 instrument (GE Healthcare). aMino sdAbs were immobilized to a CM5 sensor chip at a density of 4300-4600 RU ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered at 0.8 µm and used for purification by nickel ion affinity chromatography (GE Healthcare). A washing step in the lysis buffer supplemented with 30 mM imidazole was followed by the elution in lysis buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on an affinity chromatography column (GE Healthcare, His-Trap FF crude, #17-5286-01) with a flow rate of 1 mL/ min ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The soluble fraction was loaded on a Ni2+ affinity column (HisTrapTM Chelating HP Column, GE Healthcare, Uppsala, Sweden) using a peristaltic pump with a flow rate of 1 mL min-1 ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Biophysics 2022Quote: Tubulin from bovine brain (PurSolutions) was labeled with Cy5 NHS ester (GE Healthcare) as previously described51.
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bioRxiv - Biochemistry 2022Quote: ... 5’-FAM-labeled AP oligonucleotide was visualized on a Typhoon Trio (GE Healthcare) using excitation and emission wavelengths of 532 and 575 nm ...
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bioRxiv - Biophysics 2022Quote: ... Lectins were labeled with Cy5 mono-reactive dye (NHS ester) from GE Healthcare. Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... Probes were labeled with Cy3 or Cy5 Mono-Reactive Dye Pack (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Fluorescently labeled peptides were detected using a Typhoon Trio Plus imager (GE Healthcare) and band intensities were quantified using the ImageJ44 ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Molecular Biology 2023Quote: ... The 14C-labeled RNA bands are visualized on a Typhoon FLA900 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: The Cy5-labeled DNA bands were detected using a Typhoon9410 imager (GE Healthcare). Band intensities were quantified in Quantity One software ...
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bioRxiv - Molecular Biology 2023Quote: ... The octamer was removed from dialysis and labeled with Cy5 maleimide (GE healthcare) at H2A(K119C ...
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bioRxiv - Molecular Biology 2019Quote: ... Unreacted biotin label was removed via SEC on a Superdex 200 Increase column (GE Healthcare Life Sciences). Biotinylated BSA-Lewis X was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Biophysics 2021Quote: ... Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare, Marlborough, MA).
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bioRxiv - Systems Biology 2020Quote: ... The signal of biotin-bound HRP was visualized using Amersham ECL Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Excess biotin was removed by size-exclusion chromatography on a S200 10/300 GL column (GE Healthcare) with a distal 1 ml GSTrap 4B (GE Healthcare) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Molecular Biology 2020Quote: Affimer affinities for KRAS were determined by surface plasmon resonance (SPR) using a BIAcore 3000 (GE Healthcare Europe GmbH). Affimer proteins with a C-terminal cysteine residue were biotinylated with the biotin-maleimide (SigmaAldrich ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate was centrifuged and the clarified supernatant loaded onto a 5ml HisTrap HP nickel affinity column (GE Healthcare). dCry protein was eluted with 100 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Plant Biology 2019Quote: ... The cleared supernatant was sterile filtered and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes of lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Neuroscience 2020Quote: ... was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare). Fractions were examined on SDS-PAGE gel before pooling ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...