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Citations for GE Life Sciences :
801 - 850 of 5333 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Binding affinity of a repebody was determined through surface plasmon resonance (Biacore T200, GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then passed through a HisTrap FF Ni2+-NTA affinity column (GE Healthcare) that had been equilibrated with 10 column volumes of equilibration buffer (75 mM KxHxPO4 ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was used for affinity purification and incubated with Glutathione Sepharose 4B (GE healthcare). The beads were washed in wash buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto Ni–NTA Sepharose High Performance affinity resin (GE Healthcare Biosciences) pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2022Quote: ... Affinity constants (KD) were then calculated using BIAcore T200 evaluation software v.2.0 (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... pastoris culture medium was achieved by affinity chromatography on 5 ml HisTrapFF column (GE Healthcare; equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was isolated by affinity binding to Glutathione Sepharose® 4B resin (GE Healthcare). GST tag was then cleaved off with GST-HRV 3C protease ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded onto a StrepTap HP 1 mL affinity column (GE Healthcare) at 4°C previously equilibrated with lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... the filtered supernatant ran through a protein A and protein G affinity column (GE Healthcare) and eluted with 0.2 M citric acid at pH 3.0 and neutralized in 2 M Tris-base ...
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bioRxiv - Biophysics 2024Quote: The affinity of RCC1 for nucleosome was measured on a Biacore 8K biosensor (GE Healthcare) by surface plasmon resonance (SPR) ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel affinity chromatography columns (His-Trap HP columns, GE Healthcare, Chalfont St Giles, Bucks., UK) were used to purify the polyhistidine-tagged roGFP2-GPX fusion proteins with a gradient elution (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Immunology 2019Quote: ... reaction mixture was applied to a 1 mL HiTrap KappaSelect affinity column (GE Healthcare Life Sciences). All affinity purification steps were carried out by connecting the affinity column to a peristaltic pump with flow rate of 1ml/min ...
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bioRxiv - Molecular Biology 2020Quote: ... For Brat-NHL serial reverse affinity and heparin chromatography (HiTrap HP Heparin 5ml column – GE Healthcare) followed the affinity chromatography ...
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bioRxiv - Bioengineering 2022Quote: ... with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow, 5 mL, GE Healthcare). Samples were eluted with imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... and the supernatant was loaded onto a Ni2+ affinity column (HisTrap HP 5 ml, GE Healthcare). The column was washed with 5 column volumes (CV ...
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bioRxiv - Immunology 2021Quote: ... and the supernatant was loaded to a HisTrap Chelating HP affinity column (GE Healthcare Life Sciences) previously equilibrated with 50 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was loaded on a 5 ml HisTrap FF nickel affinity column (GE Healthcare), pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: Binding affinities were measured by isothermal titration calorimetry (ITC) using a MicroCal iTC200 system (GE Healthcare) at 25°C using wild type truncated SPPV14 as well as two mutants SPPV14 Y46A and R84A in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was isolated by affinity chromatography (5 ml HisTrap, GE Healthcare Life Science, Chicago, USA) and desalted (5 ml HiTrap ...
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bioRxiv - Microbiology 2022Quote: ... The binding affinity was determined by and curves were generated by BIAEvaluation software (GE Healthcare, USA).
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bioRxiv - Biophysics 2021Quote: ... The cleared cell lysate was applied to a HisTrap FF Ni-NTA affinity column (GE healthcare) equilibrated with HisA buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... affinity chromatography followed by a Superdex HiLoad 16/600 Superdex 75 pg column (GE Healthcare, USA) in buffer C (10mM Tris-HCl (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... S2P supernatants were passed over a HisTrap FF affinity column (GE Healthcare, Cat# 17-5255-01) and further purified using a 2 mL StrepTactin sepharose column (IBA Lifesciences Cat# 2-1201-002 ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length IgG was obtained by affinity purification utilizing a Protein A chromatography column (GE Healthcare) in AKTA avant (Cytiva) ...
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bioRxiv - Biophysics 2021Quote: ... Protein purification was performed via affinity chromatography using Ni2+ charged Fast Flow Chelating Sepharose (GE Healthcare) as stationary phase ...
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bioRxiv - Cell Biology 2019Quote: ... Purification was by successive HisTrap affinity and size exclusion (Superdex 200 10/300) chromatography (GE Healthcare). The protein concentration was determined following OD280nm measurement and calculated using an extinction coefficient of 12.49 mM−1cm−1 ...
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bioRxiv - Cell Biology 2020Quote: ... Affinity purification was carried out by passing the clarified lysate over a GSH column (GE Healthcare) using the ÄKTA Prime Plus system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 and mtEFG2 were isolated via affinity purification on HisTrap FF 5 ml columns (GE Healthcare) and standard buffers as described above ...
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bioRxiv - Biochemistry 2022Quote: ... lysate was passed through an immobilized metal affinity chromatography (IMAC) column (GE Healthcare HisTrap™ HP) equilibrated with 5 column volumes (CVs ...
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bioRxiv - Microbiology 2022Quote: ... and isolated from supernatants by affinity chromatography using Protein A or Protein G columns (GE Healthcare). MAbs were eluted with 0.1 M glycine at low pH and dialyzed into PBS.
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bioRxiv - Immunology 2022Quote: ... and the cleaved product in the flow through was subjected to kappa affinity column (GE Healthcare). The eluted product from kappa affinity column was pooled and further purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... and applied onto a HisTrap HP 5 ml affinity chromatography column (GE Healthcare, Chicago, United States) equilibrated with binding buffer (20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatant was loaded onto a 5 mL Ni2+-affinity column (HiTrap chelating column, GE healthcare), preequilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... filtered and loaded into a HisTrapTM HP affinity column (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). The protein was eluted using a 0-500 mM imidazole linear gradient.
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... then supernatant was loaded onto a 5 mL Ni2+ affinity column (HisTrap HP, GE Life Sciences) pre-equilibrated with resuspension buffer ...
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bioRxiv - Cell Biology 2020Quote: ... polymerized from a 1:20 ratio of Cy5-labeled (GE Healthcare) and unlabeled tubulin ...
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bioRxiv - Microbiology 2020Quote: ... were labeled using the Cy5 monoreactive Dye pack (PA25001, GE Healthcare) and filtered using Illustra microspin G25 columns (27532501 ...
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bioRxiv - Biochemistry 2021Quote: ... were directly labeled with Cy3 following the manufacturer’s guidelines (GE Healthcare). After 45 min incubation in the dark ...
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bioRxiv - Biochemistry 2020Quote: The N-terminus of Rad6 was labeled with Cy5 (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2022Quote: LecB was labeled with Cy3 mono-reactive NHS ester (GE Healthcare) or with biotin using NHS-PEG12-biotin (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated biotin and aggregated viruses were removed by NAP-5 gel filtration columns (GE Healthcare) and 0.2 μm pore size filters ...
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bioRxiv - Microbiology 2021Quote: ... Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare). In the SEC profile ...
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bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...