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Citations for GE Life Sciences :
901 - 950 of 1360 citations for 6 Methyl 3 pyridinecarbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... ChIP for Rat1-TAP was performed by incubating 500 μl of the solubilized chromatin sample with 20 μl of IgG Sepharose 6 Fast Flow (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
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bioRxiv - Biochemistry 2020Quote: ... The full length hTop2α was first purified by a tandem affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE healthcare) bound to Ni2+ ions followed by a StrepTrap HP column (GE healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... the eluted proteins were concentrated to ∼200 µL and purified by size-exclusion chromatography on a Superose 6 10/300 GL column (GE Healthcare) in 10 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved protein was finally purified by size-exclusion chromatography (SEC) on a Superose 6 10/300 GL column (GE Healthcare) in 25 mM Tris (pH 8) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to a gravity flow chromatography column packed with 1.5 mL of Ni Sepharose 6™ fast flow resin (GE healthcare) and incubated at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Purified a-syn was applied to High capacity endotoxin removal spin columns (Pierce, Rockford, IL, USA) and Ni Sepharose 6 Fast Flow (GE Healthcare) to remove endotoxin ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... His-tagged proteins were incubated in 1 mM DTT to keep all cysteine residues in a reduced state and subsequently immobilized on a Ni Sepharose 6 Fast Flow resin (GE Healthcare). The resin was incubated 2-4 h at 4°C with 25 nmol of each fluorophore dissolved in 1 ml of labelling buffer 1 (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Insoluble material was removed by ultracentrifugation at 150,000 x g for 20 min before purification by SEC on a Superpose 6 increase 10/300 GL column (GE Healthcare) equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 spikes were captured by StrepTactin beads (IBA) and further purified by gel-filtration chromatography using a Superose 6 column (GE Healthcare) with buffer containing 20mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... and non-deamidated (pure N373 wildtype) GII.4 P dimer species was achieved by cation exchange chromatography using a 6 ml Resource S column (GE Healthcare) at 6 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µL MkeTIR-SAVED (at least 100 µM) were injected into a size exclusion column (Superose 6 Increase 10/300 GL or Superose 12, GE Healthcare) equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 1,200 rpm at 4°C for 6 minutes and the pellet was resuspended in 5 ml of 40% Percoll (GE Healthcare) in PBS and transferred to a 15 ml tube ...
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bioRxiv - Immunology 2022Quote: ... and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P and ferritin) column (GE Healthcare) in 10 mM Tris pH=8 ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried out on a Superose 6 Increase 10/300 GL column by fast protein liquid chromatography (ÄKTA; GE Healthcare); 0.25 mM fractions were collected ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB-Fab complexes were evaluated by Native PAGE and purified on a Superose-6 column (GE Healthcare Life Sciences) into TBS pH7.4 to remove aggregates.
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and further purified with size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) in a buffer composed of 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... and the elution was concentrated to 1mL and loaded onto the size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated with 50 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction products were resolved on 6–8% (w/v) denaturing polyacrylamide gels and visualized with Typhoon FLA scanner (GE Healthcare). In total ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Immunology 2021Quote: ... followed by purification to remove unbound Fab via size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare). Complexes were diluted to 30-50 µg/mL and immediately placed on 400-mesh Cu grids and stained with 2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was concentrated using centrifugal concentrators (Amicon, 30 kDa molecular weight cut-off (MWCO)) and polished by size exclusion chromatography (SEC, Superose 6, GE Healthcare) in gel filtration buffer (30 mM HEPES/KOH pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... the NP and 1TD0-derived epitope probes were further purified by SEC on a Superose 6 10/300 GL column and a Superdex 75 10/300 GL column (GE Healthcare), respectively ...
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bioRxiv - Microbiology 2020Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al, 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The samples were further concentrated and purified by size-exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare), pre-equilibrated with SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... APH variants were purified from urea-resuspended inclusion bodies in the presence of 6 M urea on a gravity Ni-sepharose (GE Healthcare) column and eluted with the step gradient including imidazole concentrations 5 mM ...
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bioRxiv - Biochemistry 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The amounts of 6-FAM fluorescence in unextended and extended primer bands were quantitated using a Typhoon 9400 scanner and ImageQuant software (GE Healthcare). Single-nucleotide incorporation data were fit to the single exponential equation using GraphPad Prism software ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples of both male and female livers from all the different treatments (N=6-8) were individually used to isolate total RNA via the Illustra RNAspin Mini RNA Isolation kit (GE Healthcare), according to the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... concentrated using a 30 kDa molecular weight cut-off (MWCO) Ultra Centrifugal tube (Amicon) and loaded to a Superose 6-Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... After dialysis the supernatant containing folded tetramers and dimers were subjected to Superose 6 prep grade XK 16/70 size exclusion column (GE Healthcare) purification using HFB buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Biochemistry 2020Quote: Analytical gel filtration was performed on a Superdex 200 Increase 10/300 GL column or Superose 6 Increase 10/300 GL (GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... The beads were removed and the solution was further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... spun down 15 min at 15000 rpm in a bench-top centrifuge (4°C) and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare), previously equilibrated in gel filtration buffer (30 mM HEPES pH 7.5 ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Cross-linked proteins were further purified from aggregation products by size exclusion chromatography on a Superose 6 increase column (GE Healthcare) equilibrated with size exclusion buffer (10 mM HEPES pH 7.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were visualized by the 6-FAM label on the RNA primer using the Typhoon 9500 FLA Imager (GE Healthcare).