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Citations for GE Life Sciences :
751 - 800 of 1360 citations for 6 Methyl 3 pyridinecarbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Molecular Biology 2019Quote: ... concentrated with Amicon Millipore 15 ml 100,000 MWCO centrifugal concentrator and applied to Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated in storage buffer (400M KCl ...
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bioRxiv - Microbiology 2019Quote: ... Intact complexes were separated from aggregate by size-exclusion chromatography in TBS containing 0.01% (w/v) LMNG (S200 10/300 increase or Superose 6 increase, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Labeled protein was separated from unreacted fluorophore and the Sfp synthase by size-exclusion chromatography on a superose 6 3.2/200 (GE Healthcare) preequilibrated in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting filtrates were analyzed by size-exclusion column chromatography using Superose 6 Increase 10/150 GL (GE healthcare). Peak values at the retention time for the complex were plotted as a function of incubation temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The target protein was cleaved off the column with TEV protease and subjected to size exclusion chromatography on a Superose 6 Increase 3.2/300 column connected to the Ettan system (GE Healthcare) operated at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... These two complexes were then mixed together with 1.5x molar excess of Nup84-Nup133 and run over a Sepharose 6 10/300 column (GE Healthcare). Fractions containing all Y complex components were pooled and concentrated.
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bioRxiv - Biochemistry 2020Quote: ... Inc) and subsequently subjected to further purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) with the buffer of 75mM Tris (pH7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was further purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon 7000 phosphorimager (GE Healthcare) and ImageJ software (76).
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bioRxiv - Microbiology 2020Quote: ... The concentrated HDL was purified based on the difference in their molecular size by size exclusion chromatography using a Superose 6 column (1.5×60cm) (GE Healthcare) Fast Protein Liquid chromatography [V0= 34ml ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... unreacted labelling dye was removed via size exclusion chromatography (SEC) on a Superose 6 Increase column (GE Healthcare Life Sciences). All labeled proteins were flash-frozen in liquid nitrogen in the presence of 35% glycerol and stored at −80 °C until usage.
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Cell Biology 2020Quote: ... Between 3.6 and 3.8 mg of total extract was separated on a Superose 6 10/300 GL column (GE Healthcare, 17517201) operated on a NGC Quest System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... annealed and then the double stranded damaged or undamged product was purified by size exclusion chromatography (Superose 6, GE Healthcare) in buffer 300 (20 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Immunology 2022Quote: ... Additional purification was then carried out by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) with 2 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by SDS-PAGE on a 6% gel and transferred to a nitrocellulose membrane (0.45 µm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Biochemistry 2021Quote: Hexamerization of both wild-type and L396A Lon was tested by gel filtration chromatography using a Superose 6 Increase 3.2/300 column (GE Healthcare). The column was pre-equilibrated in running buffer (25 mM Tris–HCl ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Z-stacks of 6 µm (30 sections, 0.2 µm thickness each) were acquired using SoftWoRx Suite 2.0 (Applied Precision, GE). High resolution images were captured on an Elyra PS.1 super resolution microscope (Zeiss ...
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bioRxiv - Biochemistry 2019Quote: ... Base subcomplex was further purified by size-exclusion chromatography using a Superose™ 6 increase 10/300 column (GE Healthcare) pre-equilibrated with base GF buffer (60 mM HEPES pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis tagged protein was purified by IMAC affinity purification using 0.5 ml of packed Sepharose 6 Fast Flow Ni-NTA Resin (GE Healthcare) pre-washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate is then incubated overnight with 400ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) at 4°C with slow rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified U1 snRNP from the heparin column was further purified on the Superose 6 Increase 3.2/300 column (GE Healthcare) in SEC150 buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... A 50 µg sample of HELB was loaded at 0.5 mL/min onto a Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Pure proteins with the correct homooligomeric conformation were collected from a Superose 6 10/300 GL SEC column (GE Healthcare) in Tris-buffer (TBS ...
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bioRxiv - Biochemistry 2019Quote: ... The total eluate was diluted 1:2 with 25 mM Tris-HCl pH 8.0 and further purified by anion exchange chromatography using a 6 ml RESOURCE Q column (GE Healthcare) equilibrated in 95% AEX-A (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The crosslinker was inactivated with 30 mM Tris pH 8.0 and the sample was loaded onto a Superose 6 Increase 10/300 GL (GE healthcare) column equilibrated in GF buffer (30 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... the eluate was applied to a Superose 6 Increase GL size exclusion chromatography (GE Healthcare Life Sciences, Little Chalfont, UK) pre-equilibrated in BUFFER D ...
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bioRxiv - Molecular Biology 2020Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon 7000 phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were removed by centrifugation at 40,000 rpm for 30 min and supernatants were loaded onto Superose 6 Increase (GE Healthcare) for FSEC analysis.
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 33,000 g and loaded on pre-equilibrated Ni-Sepharose 6 Fast Flow (HisTrap FF, GE Healthcare). The column was washed with 20-30 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μl of the concentrated 26S proteasome sample (alone or mixed with 90 μM of model degron) was further purified by size exclusion chromatography using a Superose 6 PC 3.2/30 column (GE Healthcare), equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and concentrated for further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... equilibrated in PBS pH 7.4 and NiV-F was purified using a Superose 6 Increase 10/300 GL column (GE healthcare) equilibrated in 10 mM Tris and 150 mM NaCl pH 8.0.