Labshake search
Citations for GE Life Sciences :
851 - 900 of 966 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... The sample was then injected in an Äkta Purifier System equipped with a Superdex Peptide 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with ultrapure water ...
-
bioRxiv - Biochemistry 2021Quote: ... The reaction was performed at 37 °C for 16 h and terminated by desalting it to 50 mM ABC buffer with HiTrap Desalting column (GE Healthcare: Chicago, IL, USA). It was further purified using Ni-NTA resin and collected the flow-through to remove the side product ...
-
bioRxiv - Microbiology 2020Quote: ... the samples were resuspended in mobile phase A and filtered through a 0.45 μm nylon membrane syringe filter (GE Healthcare, Chicago, IL, United States). Mobile phase A consisted of 0.15 M triethylammonium acetate (TEAA ...
-
bioRxiv - Immunology 2020Quote: Blood samples were obtained from four healthy donors with approval of the local Ethics Committee and PBMC were isolated via density gradient centrifugation with Ficoll Paque Plus (GE Healthcare, Chicago, IL, USA). Monocytes were enriched by negative selection using a pan monocyte isolation kit (MiltenyiBiotec ...
-
bioRxiv - Microbiology 2020Quote: ... the cDNA obtained in the section “Realtime and Endpoint RT-PCR detection of deltaviruses from animal specimens” was amplified using illustra GenomiPhi V2 Kit (GE healthcare; Chicago, IL, USA). The amplified DNA was then purified with innuPREP PCRpure Kit (Analytik Jena ...
-
bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
-
bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... The blots were developed using Radiance Plus chemiluminescent substrate (Azure Biosystems, Dublin, CA, USA) and the signals quantified using the ImageQuant LAS-4000 mini Imager (GE Healthcare, Chicago, IL, USA). Primary hepatocytes were used as control.
-
bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
-
bioRxiv - Immunology 2022Quote: ... the target dsDNA from the PCR amplification was first concentrated by ethanol precipitation and then purified on an Akta Pure 150 FPLC instrument (GE Healthcare, Chicago, IL, USA) with a GE HiPrep 26/60 Sephacryl S-500 High Resolution size exclusion column and 0.3M NaCl running buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
-
bioRxiv - Physiology 2019Quote: The optical density of cell suspensions was measured at a wavelength of 600 nm using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA). The cell dry weight (CDW ...
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes and visualized by enhanced chemiluminescence using ECL Prime Western Blotting Detection Reagent (#RPN2232, GE Healthcare; Chicago, IL, USA) and imager ImageQuant LAS 4000 (GE Healthcare ...
-
bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Physiology 2021Quote: ... Membranes were again washed with 4 x 15 min TBS-T washes and a final 15 min TBS wash prior to band development with an ECL Prime Western Blot Detection Kit (GE Healthcare, Chicago, IL, USA) and imaged using a Chemidoc Imaging system (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... the samples were separated on an SDS-15% polyacrylamide gel and then electrophoretically transferred onto the nitrocellulose membrane Amersham Protran 0.45 µm NC (GE Healthcare Lifesciences, Chicago, IL, USA). Membrane-immobilized proteins were probed with mouse anti-linker histone H1 monoclonal antibody clone AE-4 (Upstate Biotechnology ...
-
bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
-
bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
-
bioRxiv - Immunology 2020Quote: ... and the size and purity of each antibody were determined by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
-
bioRxiv - Biophysics 2022Quote: ... resolved in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS-PAGE, 12% acrylamide) gels and transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA) using standard procedures ...
-
bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
-
bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
-
bioRxiv - Microbiology 2023Quote: ... The gel filtration process was performed on an Äkta Pure 25 XK 16/70 column packed with 120 ml Superdex 200 (GE Healthcare, Chicago, IL, USA), and purified proteins were eluted with 1xPBS at 1 ml/min by collecting 2 ml per fraction ...
-
bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
-
bioRxiv - Microbiology 2022Quote: ... a 1.5 cm x 50 cm (bed volume 85 mL) was packed with Sephacryl™ S-1000 superfine resin (GE Healthcare, Chicago, IL, USA) [43] and equilibrated with two bed volumes of PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
-
bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
-
bioRxiv - Biochemistry 2023Quote: ... The flow-through was collected and purified by ion-exchange chromatography on a HiTrap Q HP column (GE Healthcare Life Sciences, Chicago, IL, USA), using Buffer I1 (20 mM Tris ...
-
bioRxiv - Genetics 2023Quote: ... Then these cells were repositioned under the DeltaVision microscope (OMXTM V4; GEhealthcare, Chicago, IL, USA) and the super-resolution images were processed with SoftWoRx (Applied Precision, Issaquah, WA, USA). And the distance between axial elements was measured using IMAGEJ 1.37a software.
-
bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
-
bioRxiv - Biochemistry 2023Quote: ... thawed ASK1 TBD-CRR-KD was subjected to size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2023Quote: ... or 25 °C (ASK1 TBD-CRR and ASK1 CRR) for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
-
bioRxiv - Immunology 2023Quote: ... and processed within 2 hours by dilution at 1:1 (v/v) ratio in phosphate-buffered saline solution (PBS) with layering on top of 1 volume of Ficoll-Paque Premium (GE Healthcare, Chicago, IL, USA) and separation by density centrifugation at 500× g for 30 minutes (20°C ...
-
bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
-
bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
-
bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
-
bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
-
bioRxiv - Zoology 2019Quote: ... The gDNA concentration was estimated by Qubit and about 20 ng of gDNA amplified with primers specifically designed to include the intron region (Hsp70 genomic F/R, Table 1) using PuReTaq Ready-To-Go™ PCR Beads (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Cell Biology 2019Quote: ... and imaged using the ChemiDoc-It Imaging System and VisionWorksLS software (UVP, Upland, CA, USA) or Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL). Bands were quantified using densitometry analysis in ImageJ (Abramoff et al. ...
-
bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
-
bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in the medium for Leydig cells (low glucose DMEM (PanEco) with 10% fetal bovine serum (FBS, HyClone, GE Healthcare Life Sciences, Chicago, IL), 1% penicillin/streptomycin (PanEco) ...
-
bioRxiv - Biochemistry 2023Quote: ... USA, 1:10000), luminescence was revealed with Amersham ECL™ Prime reagents (Cytiva, UK) and acquired with ImageQuant™ LAS 4000 (GE Healthcare, Il, USA).
-
bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
-
bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
-
bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...