Labshake search
Citations for GE Life Sciences :
801 - 850 of 966 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... Eluted proteins were further purified via size exclusion chromatography using a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare, Chicago, IL) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2019Quote: Epithelial skin cells were collected from male and female volunteers using a Whatman® FTA® Sterile Omni Swab (GE Healthcare, Chicago, IL). Participants were asked to swab the sides of their nose and behind their ears for approximately thirty seconds using swabs in order to maximize skin cell yield ...
-
bioRxiv - Bioengineering 2022Quote: ... dialysed into a Tris-saline buffer (Tris 10 mM, NaCl 150 mM, pH 7.4) then affinity purified on a protein A sepharose column (GE Healthcare, Chicago, IL, USA). Bound antibody was eluted with 0.1 M Glycine pH 2.4 and neutralised with 1 M Tris pH 8.5 ...
-
bioRxiv - Immunology 2022Quote: ... mFcRn) were purified using a HisTrap Ni NTA column followed by size exclusion chromatography (Superdex 200 Increase column; both GE Healthcare, Chicago, IL) using 20 mM phosphate pH 8.0 ...
-
bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
-
bioRxiv - Physiology 2022Quote: ... and ran this mixture through handcast Tris-glycine gels prior to blotting onto nitrocellulose membranes with 0.1 µm pore size (GE Healthcare, Chicago, IL, US). Subsequently ...
-
bioRxiv - Microbiology 2023Quote: ... The blots were incubated with γ32-P 5’-labelled probe at 42°C overnight and imaged using a phosphor screen on a Typhoon FLA 7000 Variable Mode Imager System (GE Healthcare, Chicago, IL). Probe sequences are listed in Table S2.
-
bioRxiv - Neuroscience 2022Quote: ... were purified from bacterial lysate by affinity chromatography on a GSTrap FF column (Cat. No. 71-5016-96 AM, GE Healthcare, Chicago, IL) followed by dialysis against multiple changes of phosphate buffered saline (PBS) ...
-
bioRxiv - Microbiology 2022Quote: ... after which chemiluminescence reaction was conducted using Chemi-Lumi One (Nacalai Tesque) and was subsequently viewed using Amersham Imager 680 (GE Healthcare, IL, USA).
-
bioRxiv - Immunology 2022Quote: ... Both mutants carry a carboxy-terminal c-myc-peptide extension and a poly-His peptide extension for anchoring in solid phase immunoassays and purification using nickel columns from GE Healthcare (Chicago, IL) as previously described and specified below (19 ...
-
bioRxiv - Immunology 2022Quote: ... Protein was concentrated in 10 kDa molecular weight cutoff centrifugal concentrators and purified by size exclusion chromatography on an AKTAPURE FPLC (GE Healthcare, Chicago IL), using an S200 column followed by a S75 column ...
-
bioRxiv - Immunology 2022Quote: ... and purified from pre-conditioned cell media supernatant using Ni-NTA resin and size exclusion chromatography with a S200 column on an AKTAPure FPLC (GE Healthcare; Chicago, IL).
-
bioRxiv - Microbiology 2023Quote: WB was performed on a semi-dry western blot device transferred to an Amersham Protran 0.45 mm nitrocellulose (NC) blotting membrane (GE Healthcare, Chicago, IL, USA) at 52 mA for 45 min ...
-
bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
-
bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
-
bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
-
bioRxiv - Plant Biology 2023Quote: ... and their fluorescence was recorded by direct scanning of the gel using an image analyser (Typhoon 9600, GE Healthcare Bio-Science, Chicago, IL) as described (Tasaki et al ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gel was exposed to a phosphoimager screen overnight at -20°C and the phosphoimager screen was scanned using the Typhoon FLA 9000 phosphoimager (GE Healthcare, Chicago, IL). Experiments were performed in triplicate to establish reproducibility ...
-
bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples using standard Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
-
bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
-
bioRxiv - Biochemistry 2023Quote: ... The final purification step was size-exclusion chromatography on a HiLoad 26/600 Superdex 75/200 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
-
bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
-
bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
-
bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2024Quote: ... 1:3 blood dilutions were made with sterile Roswell Park Memorial Institute (RPMI) 1640 medium (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 100 unit/mL penicillin and 100 mg/mL streptomycin (Gibco Life Technologies ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
-
bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
-
bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
-
bioRxiv - Immunology 2019Quote: Healthy human monocytes were isolated from whole blood by Ficoll-Paque PLUS (17144003, GE Healthcare) density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201 ...
-
Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
-
Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
-
bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
-
bioRxiv - Microbiology 2019Quote: Isothermal titration calorimetry (ITC) experiments were performed at 20°C following standard procedures using a Microcal VP-ITC calorimeter (GE Healthcare, Chicago, IL, US). The E ...
-
bioRxiv - Developmental Biology 2020Quote: ... The membranes were washed with Tris buffered saline containing Tween 20 (AppliChem; TBST) and incubated with secondary anti-rabbit (GE Healthcare; Chicago, IL, US) or anti-mouse (GE Healthcare ...
-
bioRxiv - Physiology 2022Quote: ... 75 V) and gel image was taken using an Image Quant LAS 500 chemo luminescence CCD camera (GE healthcare, life sciences, Chicago, IL, USA).
-
bioRxiv - Molecular Biology 2020Quote: ... 250 μl were applied to an Äkta pure chromatography system equipped with a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) and run at 6 °C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Microbiology 2019Quote: ... 75-90 μl of affinity-purified protein sample was loaded onto a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare, Chicago, IL, USA) previously equilibrated with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
-
bioRxiv - Neuroscience 2020Quote: Fusion proteins of glutathione-S-transferase and mouse Clstn3 (JK091; aa 21–244) were produced in BL21 Escherichia coli and purified using a glutathione-Sepharose column (GE Healthcare, Chicago, IL, USA). Clstn1 peptides (JK025 ...
-
bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
-
bioRxiv - Biochemistry 2020Quote: ... The clear upper phase of the lysate was desalted by size exclusion chromatography using a PD MiniTrap G-25 prepacked column (GE Healthcare, Chicago, IL, USA) with assay buffer as eluent ...
-
bioRxiv - Bioengineering 2020Quote: Binding affinity characterizations of transcribed RNA biosensors were performed using a SPR assay on the Biacore X100 instrument from GE Healthcare (Chicago, IL, USA) as previously described8 ...
-
bioRxiv - Biophysics 2021Quote: ... The SΔTM protein was then further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) (Fig ...
-
bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were HRP-conjugated and bands were visualized by the ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038–560) and bands were quantified with Gel Doc system (Bio Rad ...
-
bioRxiv - Immunology 2021Quote: ... and the homogeneity and the size of the antibodies were analyzed by using an analytical Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
-
bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...