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Citations for GE Life Sciences :
851 - 900 of 2934 citations for 6 OXO 9α 11α 15S TRIHYDROXY PROSTA 13E 17Z DIEN 1 OIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The sample was separated and analysed on a 15 % SDS-PAGE gel and visualized by in-gel fluorescence on a LAS 4000 Imager (GE Healthcare, USA).
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bioRxiv - Immunology 2020Quote: ... The rest of the blood were diluted with PBS (pH7.2) to 2X of original volume and layered over 15 ml Ficoll (GE Healthcare, Waukesha, WI) in SepMate-50 tubes (Vancouver ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed again for 3 × 15 min in PBS-T and signals were detected with ECL Western Blotting Detection Reagent (GE Healthcare, #RPN2106) and acquired with Chemidoc MP Imaging System (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... The probes were run on the 15 % polyacrylamide gels and stained with Coomassie stain (Phastgel Blue R-350 from GE healthcare, US). The gel images were acquired and either Image Quant or Image J program was used for the densitometric analysis to determine the RLC exchange efficiency for the chimeric myosins ...
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bioRxiv - Molecular Biology 2021Quote: ... eIF1A preparations were re-purified on a HiTrap SP HP column and the fractions containing labeled eIF1A visualized on a 15 % SDS-PAGE using a fluorescence scanner (Typhoon™ FLA7000 (GE Healthcare)).
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bioRxiv - Biophysics 2020Quote: The reconstituted liposomes were subjected to four freeze-thaw cycles followed by extrusion 15 times using an Avanti Mini Extruder fitted with a 0.4 μm-filter (GE Healthcare, Chicago, IL). For each assay ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were obtained by centrifugation at 12,000 x g for 15 min and subjected to affinity purification on a 5 mL Excel HisTrap affinity purification column (GE Life Sciences) equilibrated with IMAC-A buffer (20 mM sodium phosphate buffer pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... All samples were loaded with the same amount of total protein (40μg) and separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (GE Healthcare, Buckinghamshire, UK) using a Transfer-Blot system (Bio-rad ...
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bioRxiv - Systems Biology 2024Quote: ... by SDS-PAGE (10 %, 15 %, 90 µg) and transferred onto nitrocellulose membranes (0.2 µm) (GE Healthcare Life Sciences, Chalfont St Giles, UK) using the semi-dry TransBlot® Turbo™ transfer system (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... SEC-MALS analysis was carried out by injecting 100 μL (A280nm = 1.0) of either sample onto a Superose 6 increase 10/300 GL (GE Healthcare) equilibrated in TBS containing 0.02% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Cell supernatant were collected after centrifuge at 40,000 rpm for 30 min and loaded onto superpose 6 increase (GE healthcare) for FSEC analysis.
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Microbiology 2019Quote: ... Intact complexes were separated from aggregate by size-exclusion chromatography in TBS containing 0.01% (w/v) LMNG (S200 10/300 increase or Superose 6 increase, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Labeled protein was separated from unreacted fluorophore and the Sfp synthase by size-exclusion chromatography on a superose 6 3.2/200 (GE Healthcare) preequilibrated in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting filtrates were analyzed by size-exclusion column chromatography using Superose 6 Increase 10/150 GL (GE healthcare). Peak values at the retention time for the complex were plotted as a function of incubation temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The target protein was cleaved off the column with TEV protease and subjected to size exclusion chromatography on a Superose 6 Increase 3.2/300 column connected to the Ettan system (GE Healthcare) operated at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... These two complexes were then mixed together with 1.5x molar excess of Nup84-Nup133 and run over a Sepharose 6 10/300 column (GE Healthcare). Fractions containing all Y complex components were pooled and concentrated.
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bioRxiv - Biochemistry 2020Quote: ... Inc) and subsequently subjected to further purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) with the buffer of 75mM Tris (pH7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was further purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon 7000 phosphorimager (GE Healthcare) and ImageJ software (76).
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bioRxiv - Microbiology 2020Quote: ... The concentrated HDL was purified based on the difference in their molecular size by size exclusion chromatography using a Superose 6 column (1.5×60cm) (GE Healthcare) Fast Protein Liquid chromatography [V0= 34ml ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... unreacted labelling dye was removed via size exclusion chromatography (SEC) on a Superose 6 Increase column (GE Healthcare Life Sciences). All labeled proteins were flash-frozen in liquid nitrogen in the presence of 35% glycerol and stored at −80 °C until usage.
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Cell Biology 2020Quote: ... Between 3.6 and 3.8 mg of total extract was separated on a Superose 6 10/300 GL column (GE Healthcare, 17517201) operated on a NGC Quest System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... annealed and then the double stranded damaged or undamged product was purified by size exclusion chromatography (Superose 6, GE Healthcare) in buffer 300 (20 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Immunology 2022Quote: ... Additional purification was then carried out by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) with 2 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by SDS-PAGE on a 6% gel and transferred to a nitrocellulose membrane (0.45 µm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Biochemistry 2021Quote: Hexamerization of both wild-type and L396A Lon was tested by gel filtration chromatography using a Superose 6 Increase 3.2/300 column (GE Healthcare). The column was pre-equilibrated in running buffer (25 mM Tris–HCl ...
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bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Z-stacks of 6 µm (30 sections, 0.2 µm thickness each) were acquired using SoftWoRx Suite 2.0 (Applied Precision, GE). High resolution images were captured on an Elyra PS.1 super resolution microscope (Zeiss ...
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bioRxiv - Biochemistry 2019Quote: ... Base subcomplex was further purified by size-exclusion chromatography using a Superose™ 6 increase 10/300 column (GE Healthcare) pre-equilibrated with base GF buffer (60 mM HEPES pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis tagged protein was purified by IMAC affinity purification using 0.5 ml of packed Sepharose 6 Fast Flow Ni-NTA Resin (GE Healthcare) pre-washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate is then incubated overnight with 400ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) at 4°C with slow rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified U1 snRNP from the heparin column was further purified on the Superose 6 Increase 3.2/300 column (GE Healthcare) in SEC150 buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... A 50 µg sample of HELB was loaded at 0.5 mL/min onto a Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Pure proteins with the correct homooligomeric conformation were collected from a Superose 6 10/300 GL SEC column (GE Healthcare) in Tris-buffer (TBS ...
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bioRxiv - Biochemistry 2019Quote: ... The total eluate was diluted 1:2 with 25 mM Tris-HCl pH 8.0 and further purified by anion exchange chromatography using a 6 ml RESOURCE Q column (GE Healthcare) equilibrated in 95% AEX-A (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The crosslinker was inactivated with 30 mM Tris pH 8.0 and the sample was loaded onto a Superose 6 Increase 10/300 GL (GE healthcare) column equilibrated in GF buffer (30 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... the eluate was applied to a Superose 6 Increase GL size exclusion chromatography (GE Healthcare Life Sciences, Little Chalfont, UK) pre-equilibrated in BUFFER D ...