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Citations for GE Life Sciences :
701 - 750 of 2934 citations for 6 OXO 9α 11α 15S TRIHYDROXY PROSTA 13E 17Z DIEN 1 OIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Molecular Biology 2022Quote: ... The complex was purified by size-exclusion chromatography on a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with buffer E (20 mM HEPES-NaOH ...
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bioRxiv - Biophysics 2022Quote: ... and 0.03% DDM (TE buffer) before anion exchange chromatography using a 6 mL Resource Q column (GE Healthcare). Nucleotide-free Tth V/A-ATPase (Vnucfree ...
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bioRxiv - Microbiology 2022Quote: Molecular weights were measured from P116 samples using a Superose 6 10/300 GL (GE Healthcare, Chicago, USA) column in a Prominence liquid chromatography system (Shimadzu ...
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bioRxiv - Molecular Biology 2022Quote: ... and 500 µL was loaded onto a Superose 6 10/300 GL column (GE Healthcare, 17-5172-01); 500-µL fractions were collected ...
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bioRxiv - Cell Biology 2022Quote: ... Clear lysate was incubated with 0.5 ml packed beads IgG Sepharose 6 Fast-Flow (GE Healthcare, Chicago, IL) for 2-3 hours at 4°C on a tube roller ...
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bioRxiv - Molecular Biology 2022Quote: ... The filtered sample was injected onto a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated in nanodisc buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were purified by size-exclusion chromatography on a Superose 6 Increase 10/300 column (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... concentrated using ultrafiltration (Amicon) and loaded onto a Superose 6 Increase 10/300 gel filtration column (GE Healthcare) equilibrated with 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Q20) were loaded into vials and automatically injected onto the column (Superose 6 3.2/300 Increase GE Healthcare) via an integrated syringe system ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Biochemistry 2023Quote: ... Samples at indicated peaks were concentrated and further purified using size exclusion chromatography (Superose 6 column, GE Healthcare) with a buffer containing 50□mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μl) for one hour on rotation was run to Superose 6 Increase 3.2/300 column (GE Healthcare) connected to Pharmacia SMART chromatography system and equilibrated with G-actin buffer (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Free RBDs were purified the next day by SEC on a Superose 6 10/300 column (GE Healthcare) and equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... The sample was further purified by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in PBS.
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
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bioRxiv - Immunology 2020Quote: ... acid-eluted immunoprecipitates were separated by SDS-PAGE under reducing conditions and transferred to HybondTM ECLTM membranes (GE Healthcare). Membranes were incubated sequentially with the different primary antibodies and developed with the appropriate HRP-conjugated secondary antibody using enhanced chemiluminescence (ECL) ...
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bioRxiv - Biophysics 2019Quote: ... its only cysteine at amino acid #265 near the flexible C terminus was labeled with Cy5 maleimide (GE healthcare) or AlexaFluor488 C5 maleimide (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Cell Biology 2020Quote: ... A 35-mL volume of diluted blood was layered over 15 mL of Ficoll-Paque Plus density gradient medium (GE Healthcare) in Leucosep 50-mL tubes (Greiner Bio-One) ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) each ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein can be further purified by size-exclusion chromatography at 15 – 20 °C on Superdex 200 10/300 GL gel filtration column (GE Healthcare) as described above.
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bioRxiv - Developmental Biology 2020Quote: The glycerol gradient was prepared in HEMK buffer (25mM HEPES, 0.1mM EDTA, 12.5mM MgCl2, 100mM KCl) using a Hoefer SG 15 gradient maker (GE Healthcare Bio-Sciences). 300µg of nuclear extract from Baf53af/-:Actin-CreER cells +/-tamoxifen was loaded onto the top of the gradient and separated by ultracentrifugation at 40,000 rpm for 16 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: Samples were electrophoresed on a 15% SDS polyacrylamide gel and proteins were then blotted onto a nitrocellulose membrane (GE Healthcare, Amersham). Nitrocellulose was blocked with 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 concentrators and further separated by HiTrap Heparin HP column and Hi-load 16/600 superdex 200 column (GE Healthcare). Fractions from the column were pooled and subjected for SEC-MALS analysis to determine their oligomeric status ...
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bioRxiv - Molecular Biology 2021Quote: Animals were anaesthetized with isoflurane and transthoracic echocardiography was performed using the GE Vivid 9 Dimension echocardiography platform with a 15 MHz i13L linear array transducer (GE Healthcare). Left ventricular (LV ...
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bioRxiv - Cell Biology 2021Quote: ... PAA gels were covered with a hydrophobic 13-mm diameter × 0.1 mm glass coverslips that were prepared fresh by coating them for 15 min at room temperature with PlusONE Repel-Silene ES (GE Healthcare) and dried with pressurised air ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Cancer Biology 2021Quote: ... was resolved using 4–15% Mini-PROTEAN® TGX™ gels and transferred to Hybond-P 0.45µm PVDF membrane (GE Healthcare). Membranes were blocked in Roche block and incubated with primary antibodies over night or for 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... were diluted with PBS and layered with 35 mL of diluted cell suspension over 15 mL of Ficoll-Paque (GE healthcare) in 50 mL conical tube ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was filtered as needed and applied to a 15 mL fast-flow DEAE or Q Sepharose column (GE Healthcare), pre-equilibrated with Buffer A (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2020Quote: The protein was then concentrated to ~15 A280 units and further purified using size-exclusion chromatography using a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA complexes were incubated at 25 °C for 15 mins before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). The peak corresponding to DNA-protein complex was collected and concentrated ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
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bioRxiv - Synthetic Biology 2020Quote: ... The eluted proteins were concentrated using Ultra-15 Centrifugal Filter Units (Amicon) and further purified by using a SuperdexTM 75 Increase 10/300 GL (GE Healthcare) size exclusion column in Tris Buffered Saline (TBS ...
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bioRxiv - Biochemistry 2019Quote: ... The exchange was terminated by addition of 15 mM MgCl2 and excess nucleotides were removed by running samples through a PD10 column (GE healthcare), or by immediate gel filtration chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... SEC to separate oligomeric species was performed using Superdex 75 10/300GL or Superdex 200 15/150GL increase columns (GE Healthcare) in SEC buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the PA was nicked by incubation with trypsin for 30 min at room temperature and the reaction was stopped by the addition of a 5-fold excess of soybean trypsin inhibitor ([15]. The nicked PA then was desalted, loaded on a HiTrap Q-column (GE Healthcare) in a buffer A and the prepore fraction was eluted by 1 M NaCl gradient followed by analysis on SDS-PAGE (Figure S1).
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were concentrated using Ultra-15 Centrifugal Filter Units (Amicon) and further purified by using a Superdex 75 Increase 10/300 GL (GE Healthcare) size exclusion column in TBS (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were concentrated to 500 µl by Amicon Ultra 15 concentrators with 30 kDa cutoff and applied to a Superdex 200 10/300 Increase gel filtration column (GE Healthcare). Individual fractions containing the NTDyn and NDD were pooled and concentrated at 10 mg/ml.
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bioRxiv - Cell Biology 2022Quote: ... A total of 15 raw images (five phases, three rotations) per plane were collected and reconstructed by using SoftWoRx software (Applied Precision), processed in ImageJ/Fiji (Schindelin et al. ...
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bioRxiv - Plant Biology 2023Quote: ... total protein extracts (10 to 20 μg) were subjected to 12% or 15% SDS-PAGE and then transferred to 0.45 μm nitrocellulose membranes (GE Healthcare, 10600003). The anti-CrATG3 polyclonal antibody was produced by injecting the recombinant wild-type CrATG3 protein into a rabbit using standard immunization protocols at the Animal Resource facility from the University of Sevilla ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM of OCP and 20 μM of FRP were pre-incubated in SEC buffer for 15 min at room temperature and then loaded onto a Superdex 200 Increase 5/150 column (GE Healthcare) pre-equilibrated with the same buffer at 0.45 ml/min ...
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bioRxiv - Molecular Biology 2023Quote: ... a membrane was washed three times with 2x SSC and 0.2% SDS at the hybridization temperature for 15 min and exposed overnight to a storage phosphor screen with subsequent scanning by Typhoon FLA 9500 (GE Healthcare).