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Citations for GE Life Sciences :
651 - 700 of 3658 citations for 3 Piperidinol 1 methyl 4 2 4 6 trimethoxyphenyl cis + since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated supernatant was loaded on a Tricorn 10/300 Sepharose 4 Fast Flow (S4FF) size exclusion chromatography column (GE Healthcare, Buckinghamshire, UK) connected to the ÄKTA prime system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Bacterial lysates were cleared by centrifugation at 10,000 g for 20 min at 4°C and applied to a HisTrap HP column using an AKTA Prime protein purification system (GE Healthcare, Life Sciences). Bound protein was eluted with a linear imidazole gradient ...
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bioRxiv - Microbiology 2022Quote: 5 μg of input and 10 μl of output was resolved by SDS-PAGE by 4-20% acrylamide gels and analyzed by in-gel fluorescence using a Typhoon FLA 7000 (GE Healthcare Life Science) using the rhodamine channel to detect TAMRA-labeled proteins.
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were centrifuged for 20 min at 23000 × g at 4°C and filtered using Whatmann Mini-Uniprep filtering vials (GE Healthcare, Buckinghamshire, UK) with a regenerated cellulose membrane (0.2-μm pore size).
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... on an Akta Pure chromatography system using a Tricorn 10/300 column with Sepharose 4 Fast Flow resin (all GE Healthcare Life Sciences). EVs were then sterilized by 0.45 μm syringe-filtration ...
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bioRxiv - Biochemistry 2024Quote: ... CezanneCAT was eluted by cleavage of the GST tag with 15 units/ml TEV protease (SigmaT4455-1KU) in PBS buffer overnight at 4°C and desalted using a PD-10 column (GE Healthcare, cat. # 17085101) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... each studied sample—the entire EC and entire MB prepared from each hemi-forebrain—was incubated with 40 µL of Protein G Sepharose 4 Fast Flow resin (GE healthcare, Piscataway, NJ) slurry (volume of settled beads:volume of storage buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM 2-mercaptoethanol and refolded on a NiFF (GE Healthcare) column using a reverse linear urea gradient with imidazole ...
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bioRxiv - Microbiology 2020Quote: ... with a Ni2+ HiTrap IMAC (2 × 1 ml) column (GE Healthcare, Poland) preequilibrated with the binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... NEC220 was passed over 2 × 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals of the transferred radioactively labelled methyl groups were detected by autoradiography using a Hyperfilm™ high performance autoradiography film (GE Healthcare) at −80 °C in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by purification via Superose 6 (GE Healthcare). The proteins were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... IgG Sepharose 6 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Immunology 2021Quote: ... or pH 6-11 (GE Healthcare, Uppsala, Sweden). With IPG strip reduction ...
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bioRxiv - Cell Biology 2020Quote: ... or IgG Sepharose (6 Fast Flow, GE Healthcare) (500 μl per 500 ml culture) ...
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bioRxiv - Microbiology 2021Quote: ... Ni Sepharose 6 Fast flow resin (GE Healthcare) affinity exclusion chromatography was performed to obtain the tagless protein (containing the tag C-terminal sequence ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA flux 6 system (GE Healthcare, USA). The system and cassette were sanitized with 1.0 N NaOH before and after diafiltration ...
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bioRxiv - Immunology 2023Quote: ... Superose 6 Increase 10/300 GL (GE Healthcare) in a mobile phase of PBS containing 300 parts per million of sodium azide and at a flow rate of 0.75 mL/min (Dionex Ultimate 3000 pump ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... filter and subjected to Superose 6 (GE Healthcare) Increase column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2024Quote: ... IMAC Sepharose 6 Fast Flow beads (GE Healthcare) was added into (Ribosome purification ...
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bioRxiv - Biochemistry 2023Quote: ... or Superpose 6 10/300 GL (GE Healthcare) size exclusion column ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-mm-diameter Whatman paper discs (GE Healthcare) were placed on top of the plate and wetted with 5 μl of a 10 mM TcmX solution in DMSO or 300 μg of erythromycin diluted in 10 μl of ethanol ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...