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Citations for GE Life Sciences :
601 - 650 of 3658 citations for 3 Piperidinol 1 methyl 4 2 4 6 trimethoxyphenyl cis + since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... Size-exclusion chromatography was performed at 4 °C using a HiLoad™ Superdex 200pg 16/600 GE preparative column (GE Healthcare, USA). The column was equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Systems Biology 2021Quote: ... the gel was washed and exposed to phosphor screen (Fujifilm Bas cassette2, Japan) for 4 hours followed by imaging with Typhoon 9210 phosphorimager (GE Healthcare, USA).
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged (21 K, 4°C, 30 min) and the supernatant applied to an AKTA Start (GE Life Sciences) at 5 mL min−1 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The lysates were then centrifuged for 20 min at 45,000 g and 4 °C and the supernatants were filtered (0.45 μM) and loaded onto HisTrap HP columns (GE Healthcare, Uppsala, Sweden). Then ...
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Evolutionary Biology 2023Quote: Native PAGE with Laemmli buffer (pH 8.8) without SDS was performed in 4–20% gradient polyacrylamide gels in a miniVE Electrophoresis System (GE Healthcare, Life Science). High molecular weight standards (Pharmacia ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: Knockdown of KRAS was achieved by gene silencing using a pool of 4 small interfering RNAs (siRNA) specific for KRAS (ON-TARGETplus SMARTpool, from Dharmacon, GE Healthcare, USA). The desired cell line was plated onto a 6-well plate and allowed to adhere (150 000 cells per well) ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun down at 14000 rpm at 4°C for 10 minutes and supernatant were incubated with GammaBind Sepharose (GE Healthcare, # 17088501) beads premixed with 1µg/mL rabbit α-mouse SorCS2 F7378 (DAKO custom-made polyclonal ab against the cytoplasmic tail of mouse SorCS2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified lysate was incubated overnight at 4 °C with 1.5 mL of buffer A-equilibrated Glutathione Sepharose® 4B (GE Healthcare, US).
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bioRxiv - Microbiology 2024Quote: ... The crude cell extract was cleared by centrifugation for 30 min at 16,000 ×rpm (4 °C) and loaded onto a 5 ml HisTrap™ HP affinity column (GE Healthcare, USA) equilibrated with buffer B ...
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bioRxiv - Immunology 2024Quote: ... using PEI-precipitation method and purified from culture supernatants by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) as previously described 15.
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bioRxiv - Molecular Biology 2024Quote: ... the gel was exposed to phosphor imager screen (Fujifilm Bas cassette2, Japan) for 4 h followed by imaging with Typhoon 9210 phosphor imager (GE Healthcare, USA) and Azure Sapphire (Azure Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... apo-pirin was incubated overnight at 4°C with 1.2 equivalents of Fe(III) chloride hexahydrate and desalted on a PD SpinTrapTM G-25 (GE Healthcare, Chicago, Illinois) into pirin storage buffer ...
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bioRxiv - Immunology 2021Quote: ... and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb; GE Healthcare Life Sciences) which were washed twice with PBS before adding to the reaction mixture ...
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bioRxiv - Immunology 2021Quote: ... and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb; GE Healthcare Life Sciences) which were washed twice with PBS before adding to the reaction mixture ...
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bioRxiv - Microbiology 2021Quote: ... Primers were labelled using T4 PNK in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol) and purified using G-50 Microspin columns (GE Healthcare, #27-5330-01). Reverse transcription reactions were stopped by adding 40 μL of cold 0.3 M Na-acetate ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Biochemistry 2022Quote: ... and size-exclusion (Superose 6; GE Healthcare) chromatography ...
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bioRxiv - Immunology 2020Quote: ... and Superose 6 (GE Healthcare Life Sciences) size exclusion chromotography (SEC) ...
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bioRxiv - Immunology 2020Quote: ... and a Superose 6 column (GE Healthcare). Before sample loading ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 increase 10/300 (GE Healthcare) was utilized for analysis of gephyrin and LS variants complexes ...
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bioRxiv - Biophysics 2023Quote: ... Sepharose 6 Fast Flow beads (GE Healthcare) were packed in a column (packed volume 10 L ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA Flux 6 (GE Healthcare, USA) were used for buffer exchange in this unit process step ...
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bioRxiv - Biophysics 2024Quote: ... and size-exclusion (Superose-6; GE Healthcare) chromatography.
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bioRxiv - Molecular Biology 2023Quote: ... The signals of the transferred radioactively labelled methyl groups were detected by autoradiography using a HyperfilmTM high performance autoradiography film (GE Healthcare) at −80LJ°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The affinity tag for Munc18 was cleaved by 2 mg of TEV in PBS with 400 mM imidazole overnight at 4℃ and additional purification was performed using superdex 200 10/300 GL (GE Healthcare, Piscataway, NJ) gel filtration column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag with the 3C protease (MERCK) ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were exposed to a phosphor screen for a minimum of 4 hours and scanned using a Storm 820 phosphorimager (GE Healthcare Life Sciences). The bound fraction of each DNA substrate was plotted as a function of PolY concentration and fit to Equation 2 to obtain the dissociation constant ...
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bioRxiv - Biochemistry 2020Quote: ... Resolved products were exposed to a phosphor screen for a minimum of 4 hours and scanned with a Storm 820 phosphorimager (GE Healthcare Life Sciences). The product signals were quantified using ImageQuant software (v5.0 ...
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bioRxiv - Plant Biology 2021Quote: ... GFP or mCherry fluorescence was measured 4-5 days post-infiltration (see Table S3 for raw data) using the ImageQuant LAS 4000 luminescent imager (GE Healthcare Life Sciences) with the appropriate excitation and emission settings (EX ...
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bioRxiv - Biochemistry 2021Quote: ... or by sonication and cleared lysate was obtained by centrifugation (24,446 x g; 30 min; 4°C) and filtration using a Whatman™ folded filter (GE Healthcare, now Cytiva).
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bioRxiv - Molecular Biology 2020Quote: ... Sample was washed twice with HBSS and then centrifuged for 15 minutes at 1800 RPM at 4° C through 25% Percoll (GE HealthCare, Pittsburg, PA). The resulting fatty upper and fluid middle layers were removed from the pellet ...