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Citations for GE Life Sciences :
601 - 650 of 4132 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Clarified lysate was passed over a 1-ml GSTrap Fast Flow Column (GE Healthcare) equilibrated with binding/wash buffer (20 mM phosphate pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... was bound to a 1 ml HiTrap® NHS- activated HP column (GE Healthcare) according to manufacturer’s guide ...
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bioRxiv - Biochemistry 2023Quote: ... The septa were pooled and digested with 1 ml of 0.25% trypsin (GE Healthcare) and 0.5 ml of 100 kU DNase I (GE healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µL of sample were injected on a Superose 6 30/100 (GE Healthcare) analytical SEC column preequilibrated with the last dialysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
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bioRxiv - Genomics 2019Quote: ... The bioreactors were inoculated at a target cell density of 0.4 × 106 cells/mL in ActiPro batch media and fed daily beginning on Day 3 (3% (v/v) Boost 7a and 0.3% (v/v) Boost 7b (GE Healthcare)) ...
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bioRxiv - Biophysics 2023Quote: Sephadex G-100 (GE Healthcare) was equilibrated in 10 mM sodium phosphate (pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mL fractions corresponding to ∼10 mL of the elution peak were pooled and applied to a 1 mL StrepTrap column (GE Healthcare, #28-9075-46) pre-equilibrated with Buffer N with 2 mM DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... and subcloned into pGEX-4T-3 (GE Healthcare) using BamHI and SalI ...
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bioRxiv - Bioengineering 2020Quote: A solution of 25 mg/L SC-SWCNTs and 2 mg/mL C16-PEG2k-ceramide in 1% sodium cholate was added to a 1 kDa molecular weight cutoff dialysis cartridge (GE Healthcare). The solution was dialyzed against water with multiple water exchanges for a week ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3 and 3:1 (w/w) at a concentration of 1 mg/mL were separated on a Superdex 200 10/30 column (GE Healthcare) using a LC1100 HPLC System (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µL TIR-SAVED (10.9 mg.mL-1) were injected into the Superose 12 column 10/300 GL (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 mM DTT) and purified by size-exclusion chromatography in a HiLoad 26/100 Superdex 200 column (GE Healthcare) pre-equilibrated with gel filtration buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare). For the experiments presented in Figure S2A and S2B another DeltaVision microscope equipped with a CoolSNAP-HQ2 cooled CCD camera (Photometrics ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Obtained gels and membranes were analyzed in Image Master 2D Platinum 7 Software (GE Healthcare, Chicago, IL, USA) to look for qualitative and/or quantitative differences in abundance/antigenicity of some venom proteins between the samples ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... The filtered lysate was loaded onto a 1 ml HisTrap FF (GE Healthcare, Freiburg, Germany) and unbound protein was removed with 20 column volumes of 15% buffer B (50 mM HEPES/KOH pH 7.8 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM benzamidine) and loaded on a 5 ml HiTrap IMAC HP column (GE Healthcare). After a wash step for 3 column volumes in 30% IMAC buffer B (same as IMAC A but 500 mM imidazole) ...
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bioRxiv - Biophysics 2022Quote: ... to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare) column equilibrated with buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was then loaded on a 1 mL HisTrap FF Crude column (GE Healthcare) with flow rate of 1 mL/min using the AKTA FPLC ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant (crude extract) loaded on a 1-ml His-Trap column (GE Healthcare) previously equilibrated with Buffer H ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was loaded onto a 1 ml HiTRAP DEAE column (GE Healthcare, Boston, MA) for ion exchange chromatography using an GE Akta Purifier (buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified extract was applied to a 1 mL HisTrap HP column (GE Life Sciences) on an AKTA FPLC (Amersham Pharmacia) ...
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bioRxiv - Microbiology 2020Quote: ... using multiple runs over a 1 ml Protein-A HiTrap column (GE Healthcare Life Sciences) on an ÄKTA FPLC (GE Healthcare Life Sciences) ...
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bioRxiv - Immunology 2020Quote: ... at a flow rate of 1 ml/min using an EttanLC HPLC system (GE Healthcare) with buffer A (0.1 % trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2022Quote: ... to be separated with two in-parallel 1-mL HisTrap FF crude columns (GE Healthcare). The column was washed with lysis buffer supplemented with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... The dialyzed samples were subsequently loaded onto a 1 mL StrepTrap HP column (GE Healthcare), which was washed with 20 CV of binding buffer ...
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bioRxiv - Microbiology 2022Quote: ... and loaded onto an ion exchange column (HiTrap™ Q HP 1 mL, GE Healthcare) equilibrated with 0.05% LDAO ...
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bioRxiv - Microbiology 2022Quote: ... were loaded onto an ion exchange column (HiTrap™ Q HP 1 mL, GE Healthcare) equilibrated and washed in the same buffer ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded onto a StrepTap HP 1 mL affinity column (GE Healthcare) at 4°C previously equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1 mL with buffer B and added immediately onto an anion exchange resin (Q resin; GE Healthcare; 0.2 mL; equilibrated in buffer H) and incubated (5 min) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Neuroscience 2022Quote: ... distilled water was replaced with a 1:1 dilute of non-ionic iodine Omnipaque 350 contrast agent (Iohexol, 350 mg iodine/ml; GE Healthcare AS) in normal saline via 3 ml syringe ...
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bioRxiv - Immunology 2023Quote: ... to a ratio of 1 to 1 before being layered onto Ficoll Paque (1.077 g/ml; GE Healthcare Europe GmbH, Freiburg, Germany). After centrifugation (800 x g for 25 min) ...
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bioRxiv - Cell Biology 2019Quote: ... adjusted to 100 mM Tris-HCl pH 8.0 and 1 mM EDTA and loaded onto a StrepTrap HP column (GE Healthcare) pre-equilibrated in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...