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Citations for GE Life Sciences :
6151 - 6200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was passed through a HiLoad® 26/600 Superdex® 75 pg (GE Healthcare) size exclusion chromatography column and the resulting pure protein was stored in gel filtration buffer ...
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bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... 30 mM NaCl (buffer A) the supernatant was loaded onto a Mono S cation exchange chromatography column (GE Healthcare) equilibrated with buffer A using a ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Excess dye was removed by SEC on S200 16/600 column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer was exchanged via a PD10 column (GE Healthcare). For tag cleavage ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Cell Biology 2023Quote: ... equal volumes (125 μl containing 6250 µg) of two different kinds of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated with secondary ECL anti-rabbit or anti-mouse IgG HRP-linked antibodies (GE Healthcare, Pittsburgh, PA) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Plasma was separated from the entire blood by a density gradient centrifugation using the Ficoll-Paque™ PLUS (GE healthcare, Chicago, IL, USA), and plasma aliquots were stored at −80°C.
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bioRxiv - Cancer Biology 2023Quote: ... for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare, Velizy-Villacoublay, France) in cold blotting buffer (25mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The peak associated with pure ACBP was concentrated and further purified by SEC using a Superdex 75 (HR 10/30) column coupled to an Äkta purifier system (GE Healthcare) using Buffer A ...
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bioRxiv - Biophysics 2023Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: Tubulin from bovine brain (PurSolutions) was also labeled with Alexa 568 NHS ester (GE Healthcare) as previously described(58).
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% fetal bovine serum (FBS, Heat Inactivated, GE Healthcare, IL, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 mL of Amersham™ ECL Prime Western Blotting Detection Reagent (GE HealthCare) was added to each membrane in the dark for 1 min ...
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bioRxiv - Bioengineering 2023Quote: ... Further purification was done in 96 well microplates using Capto Q AEX-resin (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... transferred to a nitrocellulose membrane (AmershamProtran 0.45 µM NC; GE Healthcare Life Sciences), and blocked in 5 % BSA at room temperature for 1h ...
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bioRxiv - Bioengineering 2023Quote: ... Amersham Hyper film (GE Healthcare) was used for capturing the chemiluminescent signals.
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated Hsp10 preps were first diluted tenfold with chromatography buffer before loading into a HiPrep SP XL 16/10 column (GE Healthcare). A sodium chloride linear gradient from 0 to 1 M was used to purify Hsp10 and it eluted at a conductivity of 13.2 mS/cm ...
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bioRxiv - Biochemistry 2023Quote: ... Hsp10 fractions were pooled and concentrated using a Vivaspin 20 MWCO 10,000 spin column before purification on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... and deletion mutant preps were first diluted tenfold with chromatography buffer before being loaded through a Hi Trap Q XL column (GE Healthcare). A linear gradient of sodium chloride from 0 to 1 M was used to purify the chaperonins where each eluted at a conductivity of 5.5 mS/cm ...
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bioRxiv - Biochemistry 2023Quote: ... The evaporated solution was centrifuged as described above and applied to a QAE Sephadex A25 column (GE Healthcare) equilibrated with 500 ml of 50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA was blotted onto a nylon membrane (Amersham Hybond-N+; GE Healthcare, Chicago, IL, USA) and hybridized with DIG-labeled probe using DIG Easy Hyb (Roche Diagnostics ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mM Magnesium acetate and 0.01% Tween 20) and compared to the activity with 100 µM of the previously identified substrates dGTP (GE Healthcare, 27-1870-04) for NUDT15 and 8-oxo-dGDP (Jena Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... The synthesized DNA products were separated on denaturing 30% PAGE with 7 M urea in 1xTBE and visualized on a Typhoon 9400 (GE Healthcare, USA).
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bioRxiv - Biochemistry 2023Quote: ... L proteins then used for single-particle cryo-EM were further purified by size-exclusion chromatography using a Superdex 200 column (Cytiva, formerly GE Life Sciences) in buffer B ...
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bioRxiv - Biochemistry 2023Quote: ... followed by imaging on Typhoon 9400 (GE Healthcare, USA). Experiments were repeated three times ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated Cosmic Calf Serum (cat#SH30087.03, GE Life Sciences), 25 mM HEPES pH 7.2 (cat#25-060-CI ...
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bioRxiv - Biochemistry 2023Quote: ... Cleared supernatant was mixed with Ni-NTA agarose (GE Healthcare, MO), bound overnight with mixing ...
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bioRxiv - Bioengineering 2023Quote: Phototransduction activity was assessed by measuring the light-induced hydrolysis of endogenous cyclic (c) GMP with an enzyme immunoassay kit (Biotrack EIA system) according to the manufacturer’s instructions (Amersham Bioscience GE Healthcare). Undifferentiated WTCAct or WTCUDC iPSC and cells differentiated into RS were kept in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins in the supernatant were further purified by HiTrap Heparin HP column (GE Healthcare) with A Buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2023Quote: ... pGEX-2T (GE Healthcare, N-terminal fusion with GST) was used for CPK protein production in bacteria for previously reported constructs72 (CPK2/3/5/11) ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Plant Biology 2023Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity IMAC was performed by batch binding the clarified supernatants with 10mL of Ni Sepharose Excel resin (GE Healthcare). After 20-30 minutes of incubation ...
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bioRxiv - Plant Biology 2023Quote: ... Immunochemical detection was performed using an enhanced chemiluminescence detection reagent (ECL Prime, GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...
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bioRxiv - Biochemistry 2023Quote: Protein samples within the concentration range 0.5-1 mg/mL were injected onto pre-equilibrated Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were electrophoresed for 35 minutes at 150 V in 1X Tris-Borate-EDTA (TBE) buffer and the bands were visualised using a Typhoon scanner (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... and imaged on a Typhoon variable mode scanner (GE Healthcare) for fluorescein fluorescence.
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bioRxiv - Biochemistry 2023Quote: ... The sample was further purified using a Superdex 200 increase column (GE Healthcare) and eluted using the binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... SC and the D1 domain of FcμR was first immobilized on the chip: SC was biotinylated at the C-terminal and immobilized on the SA chip (GE Healthcare), whereas FcμR-D1 was immobilized on the CM5 chip to a level of resonance units similar to pIgR ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... samples were desalted into storage buffer (50mM Tris [pH 8.0], 0.2M NaCl, 0.5mM EDTA) using PD MiniTrap Sephadex G-25 columns (GE Healthcare). Otherwise ...
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bioRxiv - Biochemistry 2023Quote: ... Images were acquired and quantitated with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... foetal bovine serum (104.9 mL·L−1; HyClone™; GE Healthcare Life Sciences; Utah; USA), penicillin-streptomycin 100× solution (10.5 mL·L−1 ...
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bioRxiv - Biochemistry 2023Quote: ... foetal Bovine Serum (104.9 mL; HyClone™; GE Healthcare Life Sciences; Utah; USA), penicillin-streptomycin 100× solution (10.5 mL·L−1 ...