Labshake search
Citations for GE Life Sciences :
6051 - 6100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Images were analyzed by the Typhoon Imaging System (GE Healthcare) and quantified by Image Lab (Bio-Rad) ...
-
bioRxiv - Biochemistry 2023Quote: A Superdex 75 increase 10/300 GL column (GE Healthcare) was equilibrated with running buffer (150 mM NaCl ...
-
Balanced Interplay Between Hsp110, Hsp70 and Class B J-Domain Protein Improves Aggregate DisassemblybioRxiv - Biochemistry 2023Quote: ... followed by anion exchange chromatography using Q-Sepharose (Q Sepharose Fast Flow, GE Healthcare). Luciferase-GFP (1,3mg/mL ...
-
Balanced Interplay Between Hsp110, Hsp70 and Class B J-Domain Protein Improves Aggregate DisassemblybioRxiv - Biochemistry 2023Quote: ... An excess of the label was removed with a desalting column (PD-10, GE Healthcare). The BLI experiment was performed as with unlabelled proteins and the fluorescence of the protein dissociated into the buffer A with 5 mM ATP 1 mM DTT was measured using Beckman Coulter DTX 880.
-
bioRxiv - Bioengineering 2023Quote: ... 10% v/v FBS (HycloneTM, GE Healthcare, USA), 1% v/v anti-anti (15240 ...
-
bioRxiv - Bioengineering 2023Quote: ... v/v) and was loaded onto a HiTrap Capto Q column (GE Healthcare). Further purification involved a NaCl gradient (0–1000 mM ...
-
bioRxiv - Cancer Biology 2023Quote: ... MNCs were isolated from each sample using the standard gradient separation approach with Ficoll-Paque PLUS (GE Healthcare Lifesciences, Pittsburgh, PA).
-
bioRxiv - Biophysics 2023Quote: ... The 35S-methionine and 35S-cysteine incorporation into the core- and complex-glycosylated CFTR variants were visualized by fluorography and quantified using phosphorimage analysis on a Typhoon imaging platform (GE Healthcare). CFTR radioactivity was quantified after background subtraction ...
-
bioRxiv - Biophysics 2023Quote: ... followed by gel filtration chromatography on a Superdex 200 16/60 column (GE Healthcare), in 50 mM Tris pH 7.4 ...
-
bioRxiv - Biophysics 2023Quote: ... The eluate was subjected to size-exclusion chromatography on a Superdex 200i 300/10 (GE Healthcare Life Sciences) in a buffer containing 50 mM NaH2PO4/Na2HPO4 ...
-
bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
-
bioRxiv - Cancer Biology 2023Quote: ... Autoradiography and western blot were quantified using the Typhoon Imager (Image QuantTool, GE healthcare).
-
bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
-
bioRxiv - Biophysics 2023Quote: ... and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare) with Tris-NaCl buffer (50 mM Tris ...
-
bioRxiv - Biophysics 2023Quote: ... The collected supernatant was then loaded on a pre-equilibrated Ni-NTA column (GE healthcare Bio-Science AB, Sweden). The column was subsequently washed with 30 column volumes (CV ...
-
bioRxiv - Biophysics 2023Quote: ... The eluted sample was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and purified by Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2023Quote: ... which was removed by anion-exchange purification using HiTrap Q HP column (GE Healthcare). HP1α was further purified through size-selection using a Superdex-75 16/60 size-exclusion column (GE Healthcare) ...
-
bioRxiv - Biophysics 2023Quote: ... The resulting sample was applied to a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with 50 mM Hepes-HCl (pH = 7.5) ...
-
bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
-
bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, GE Life Sciences) and penicillin-streptomycin solution (P/S ...
-
bioRxiv - Biophysics 2023Quote: ... concentrated and subjected to size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 (GE Healthcare) column ...
-
bioRxiv - Biophysics 2023Quote: ... The labeled BRICHOS was separated from unreacted label by a PD-10 column (GE Healthcare) equilibrated with PBS ...
-
bioRxiv - Biophysics 2023Quote: ... the supernatant was filtered (0.22 μm pore size) and applied to a HisTrap HP 1 mL column (GE Healthcare) for affinity chromatography ...
-
bioRxiv - Biophysics 2023Quote: ... was loaded onto the Superdex 75 16/60 column (GE Healthcare) for gel filtration ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed three times with PBS for 10 minutes and subjected to signal detection using ECL™ Prime Western Blotting System (Cytiva) and ImageQuant™ LAS 4000 mini (GE HealthCare). The primary and secondary antibodies were diluted with a PBS solution containing 1% non-fat milk.
-
bioRxiv - Biophysics 2023Quote: ... and loaded onto a Superdex 200 Hiload 16/600 (GE Healthcare) in 20 mM HEPES pH 8.0 ...
-
bioRxiv - Biophysics 2023Quote: ... and Superdex 200 10/300 GL column (GE Healthcare). Elf1core was purified in the same manner as wild-type protein ...
-
bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
-
bioRxiv - Biophysics 2023Quote: ... and buffer exchanged into a final buffer (20 mM Tris, pH 7.4, 500 mM NaCl) using a HiPrep Desalting column (GE Healthcare). The purified protein was flash-frozen in liquid nitrogen and stored at -20α C.
-
bioRxiv - Biophysics 2023Quote: ... and Mono Q anion exchange chromatography columns (GE Healthcare). Pol II was purified by incubating 10-subunit Pol II with 3-fold of Rpb4/7 followed by gel filtration ...
-
bioRxiv - Biophysics 2023Quote: ... and Superdex 200 10/300 GL columns (GE Healthcare). Rad26 mutants were purified in the same manner as wild-type proteins ...
-
bioRxiv - Biophysics 2023Quote: ... The resulting suspensions were centrifuged (12.000 rpm for 35 min at 4α C) and the supernatants were purified through a HisTrap HP column (GE Healthcare) previously equilibrated with lysis buffer ...
-
bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
-
bioRxiv - Biophysics 2023Quote: ... and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare), and Superdex 200 10/300 GL column (GE Healthcare) ...
-
bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... and loaded into a Superdex 200 16/600 column (GE Healthcare) equilibrated with gel filtration buffer (50 mM K.HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.5x TBE buffer) and bands were visualized on a Typhoon FLA 9500 laser scanner (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
-
bioRxiv - Biochemistry 2023Quote: ... Affinity purification was carried out using a high affinity anti-GFP nanobody covalently coupled to agarose beads (NHS-activated beads, GE Healthcare) in a 3:1 ratio (mg:ml) ...
-
bioRxiv - Biochemistry 2023Quote: ... The concentrated sample was then purified by gel filtration on a Superose-6 Increase column (GE Healthcare) equilibrated with the same buffer used for the buffer exchange ...
-
bioRxiv - Biochemistry 2023Quote: ... Magnetic Ni-Sepharose beads (GE Healthcare) were washed in Promega buffer containing 0.2% BSA ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatant was mixed with 20 µL GSH-Sepharose beads (GE Healthcare 17-5132-01) and incubated overnight at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.8 µl of nickel magnetic Sepharose beads (28-9799-17, GE Healthcare; pre-blocked with 25 mM Tris-HCL ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered at 0.8 µm and used for purification by nickel ion affinity chromatography (GE Healthcare). A washing step in the lysis buffer supplemented with 30 mM imidazole was followed by the elution in lysis buffer supplemented with 500 mM imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... loaded on a 1 ml heparin column (GE Healthcare) and eluted in the heparin buffer supplemented with 500 mM NaCl ...
-
bioRxiv - Biophysics 2023Quote: ... and the filtered supernatant loaded onto a HisTrap HP affinity column (GE Healthcare), and the protein eluted using an imidazole gradient ranging from 25-500 mM ...
-
bioRxiv - Biophysics 2023Quote: ... The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0, 0.15 M NaCl) using a HiPrep 26/10 column (GE Healthcare Life Sciences). The cleavage reaction was initiated by adding 6×His-tagged SUMO protease to a final concentration of ∼8 μg/mL to the protein solution ...
-
bioRxiv - Biophysics 2023Quote: ... Concentrated protein was then subjected to single step buffer exchange with Dialysis Buffer (20mM Tris-Cl, pH 8.0, 50mM NaCl) with the help of aPD10 desalting column (GE Healthcare, Sephadex G-25 M matrix) under gravity flow ...
-
bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
-
bioRxiv - Biophysics 2023Quote: ... Anti-GFP nanobodies were conjugated with CNBr Sepharose beads (GE Healthcare, #17-0430-02). The resin was added to the cleared supernatant at a ratio of 1 ml of resin per 10 g of cell powder and incubated at 4 °C for 3 h with gentle shaking ...