Labshake search
Citations for GE Life Sciences :
551 - 600 of 4725 citations for Nipah Virus Glycoprotein G Recombinant Protein Human Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
-
bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
-
bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
-
bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
-
bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
-
bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
-
bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
-
bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
-
bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3C protease was removed by Ni-chelating sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
-
bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS; Hyclone, GE Healthcare), 50 μM 2-mercaptoethanol ...
-
bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
-
bioRxiv - Plant Biology 2022Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 µl 0.5 mM NiCl2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
-
bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
-
bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
-
bioRxiv - Cell Biology 2019Quote: Co-immunoprecipitations of Rps3p with Bfr1p-3HA were performed with similar conditions to Bfr1p-yeGFP but immunoprecipitated with Protein G Sepharose® 4 Fast Flow beads (GE Healthcare). Prior to the immunoprecipitations ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were washed once with PBS containing 0.05% Tween-20 (PBST) and then incubated with Protein A/G Sera-Mag SpeedBeads (GE Life Sciences, 17152104010350) for 1 hour at 4°C with orbital shaking ...
-
bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
-
bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
-
bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
-
bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
-
bioRxiv - Developmental Biology 2022Quote: ... or normal rabbit serum (Abclon, Seoul, Republic of Korea) was cross-linked with Protein G Mag Sepharose beads (GE Healthcare, IL, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: Total IgG antibodies were captured from 2 µL of plasma using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Free proteins were labeled following the manufacturer’s instructions and purified using Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences).
-
bioRxiv - Immunology 2020Quote: ... Clarified Expi293F culture supernatants were filtered and Fabs were purified by successive affinity and size-exclusion chromatography (on HiTrap Protein G and Superdex 200 Increase 10/300 GL columns, respectively, from GE Life Sciences) using an AKTA pure system.
-
bioRxiv - Immunology 2020Quote: ... 1 ml of the collected plasma was heat-inactivated (56°C for 40 min) and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
-
bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
-
bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Chromatin-IgG complexes were pull down using protein A/G sepharose beads (GE Healthcare; Cat #17-0618-01 and #17-0780-01) for 2h rocking at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
-
bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS) (GE Healthcare Life Sciences) and 1% (v/v ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS) (GE Healthcare Life Sciences), 10 mM HEPES pH 7.4 ...
-
bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) foetal calf serum (FCS, Gibco or GE Healthcare) and 100 U/ml penicillin and 100 µg/ml streptomycin (P/S ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, GE Healthcare and Eurobio), pyruvate sodium 1mM (Life Technologies) ...
-
bioRxiv - Microbiology 2021Quote: ... The buffer was replaced using G-25 columns (PD MidiTrap G-25, GE Healthcare) with 25 mM Tris-HCl ...
-
bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
-
bioRxiv - Systems Biology 2019Quote: ... Lysates (10-40 μg/lane) were separated by SDS-PAGE and protein was transferred onto a polyvinylidene fluoride (PVDF) membrane (Hybond-P, GE Healthcare, Uppsala, Sweden). Specific primary antibodies (see Antibodies subsection ...
-
bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We pooled fractions containing the purified protein, desalted the pooled fractions using PD-10 desalting columns packed with Sephadex G-25 resin (17085101, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
-
bioRxiv - Neuroscience 2020Quote: ... The remaining elution buffer from the protein purification process was substituted by PBS using sephadex-based PD MidiTrap G-25 columns (GE Healthcare, Chicago, USA). Afterwards ...
-
bioRxiv - Neuroscience 2019Quote: ... and 140 mM NaCl) was added and samples were pre-cleared for 1 hour using protein G sepharose (GE Healthcare, 17-0618-01). Immunoprecipitation was carried out overnight at 4 °C using anti-HA agarose beads (Sigma ...