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Citations for GE Life Sciences :
801 - 850 of 4725 citations for Nipah Virus Glycoprotein G Recombinant Protein Human Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and purified by the ProbeQuant G-50 micro columns kit (GE Healthcare). Probes were stored at -20°C.
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bioRxiv - Cell Biology 2020Quote: ... protein A Sepharose (GE healthcare) bead pre-equilibrated with IP dilution buffer was added and nutated further for 2 hr at 4°C for immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... protein A-sepharose (GE Healthcare) beads were added to the cell lysate-antibody mixture and incubated at 4°C for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... or protein A (GE Healthcare) (for IgG ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...
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bioRxiv - Plant Biology 2021Quote: Molecular masses of recombinant GgNAD-MEs were evaluated by gel-filtration chromatography on an ÄKTA purifier system (GE Healthcare) using a Biosep-SEC-S3000 column (Phenomenex) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hemolysate supernatants and purified recombinant hemoglobins were similarly desalted by passing through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were lysed and recombinant KRAS G12D was purified using a HisTrap HP (GE Healthcare, 29-0510-21) column ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare). Total protein was induced by isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2020Quote: Protein-Protein interaction measurements were carried out on a BIACORE X100 (GE Healthcare Life Sciences). Sensorgrams were obtained as single cycle kinetics runs ...
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bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Mss116 protein was further purified by fast protein liquid chromatography (FPLC) Superdex 200 (GE Healthcare), the protein purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... Sample protein masses were assessed with Amersham high molecular weight protein marker mix (GE Healthcare) with proteins porcine thyroglobulin (Rs=17.0nm) ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Bioengineering 2023Quote: For all protein variants a protein A chromatography (MabSelect SuRe resin, GE Healthcare, Uppsala, Sweden) was used as capture step ...
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinants showing intense hybridization signal selected for the plasmid extraction using the IllustraTM plasmidPrep Mini Spin Kit (GE Healthcare). The inserts released by the EcoRI (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The purified EGFR recombinant extracellular domain was first coupled to the surface of a CM5 sensor chip (GE Healthcare Biosciences)) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The dsRNA probe was purified by using G-25 MicroSpin column (GE Healthcare). Geiger–Muller counter was used to detect the transfer of radio-label.
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bioRxiv - Molecular Biology 2022Quote: ... 5% glycerol) using PD SpinTrap G-25 columns (GE Healthcare #28-9180-04) pre-equilibrated with conjugation buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... unincorporated nucleotides were removed using MicroSpin G-25 columns (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2021Quote: Co-immunoprecipitation: GammaBind G Sepharose beads (50ul/sample; #17-0885-01, GE Healthcare) were washed in 1xPBS (pH7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Riboprobes were purified using G-50 spin columns (GE Healthcare, Waukesha, WI, USA). The plasmid containing nearly full-length cDNA of the arc transcript was used to generate probes.
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bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Unreacted drug was removed using an Illustra G-25 spin column (GE Healthcare) equilibrated in TE buffer (10 mM Tris• HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... and then probes were purified using Illustra Microspin G-50 columns (GE Healthcare) to remove unincorporated nucleotides ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions were passed twice through a Sephadex G-25 desalting column (GE Healthcare) to get rid to free dUTP and subsequently bound to Dynabeads M-280 Streptavidin (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... The free dNTPs were removed on a Sephadex G-25 column (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... the reaction mixture was subjected to PD-Minitrap G-25 column (GE Healthcare) to remove unreacted dyes according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated nucleotides were then removed using Illustra MicroSpin G-50 columns (GE Healthcare) and the DNA isolated via phenol-chloroform extraction with Phase Lock Gel Tubes as per the manufacturer’s instructions (5PRIME) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM EDTA over a Sephadex G-25 PD-10 column (GE Healthcare). After diluting to a final volume of 5 mL ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.2) using PD-10 desalting prepacked columns (Sephadex G-25M, GE Healthcare) following manufactures instructions.
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bioRxiv - Microbiology 2020Quote: ... the reaction mixture was subjected to PD-Minitrap G-25 column (GE Healthcare) to remove unreacted dyes according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 100 µl peroxidase-conjugated goat anti-mouse immunoglobulin G (IgG) (GE Healthcare), IgG1 ...
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bioRxiv - Microbiology 2021Quote: ... TCEP was removed with a PD SpinTrap G-25 desalting column (GE Healthcare) equilibrated with reaction buffer (25 mM HEPES pH 7.6 ...
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bioRxiv - Immunology 2022Quote: ... The mixture was then applied to a G-25 desalting column (GE Healthcare) to separate the radioiodinated S from unreacted radioiodine ...