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Citations for GE Life Sciences :
551 - 600 of 3098 citations for Anti Cullin 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was clarified by centrifugation at 13000 rpm for 10 min at 4°C by a table-top centrifuge and further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in chromatography buffer (20 mM HEPES–KOH pH 7.6 ...
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bioRxiv - Microbiology 2023Quote: nFhGST was purified by affinity chromatography from a Fasciola hepatica adult fluke extract using a 5/5 GSTrap HP column (GE Healthcare) following a pre-established protocol9 ...
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare). To detect the deGRadFP expression from the hb-deGRadFP construct ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Pathology 2021Quote: ... horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare). Each signal was detected using Immunostar Zeta or LD (Fuji film ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (GE Healthcare) were used at 1:5,000 for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Scp160 antibodies (Gift of R. Jansen, U. Tuebingen) was performed using the Amersham ECL Western Blotting Detection Kit (GE Healthcare Life Sciences). Quantification of protein bands was performed using ImageJ software.
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bioRxiv - Developmental Biology 2021Quote: ... The bound protein was detected using rabbit anti-GH antibody at a final dilution of 1:40,000 (AFP5672099, NIDDK-NHPP) followed by horseradish peroxidase-conjugated antibody (anti-rabbit, IgG; GE Healthcare, Chicago, Illinois, USA) at a final dilution of 1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed to remove unbound phages and then probed with anti-M13-HRP secondary antibody (1:5000, Cytiva [formerly GE Healthcare], Marlboro, MA) followed by TMB as noted above.
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Biochemistry 2023Quote: ... H2AX (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were imaged using Typhoon 5 Imager (GE Healthcare) to record signals from the Cy5 dye ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare) was used for further purification which resulted in a monodisperse and clear INO80 complex ...
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bioRxiv - Biophysics 2019Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and finally eluted with 220 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... on a MonoQ 5/50 GL column (GE Healthcare). Sample purification and analysis with HPLC were performed using Agilent 1260 Infinity (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl of streptavidin beads (GE Healthcare, Chicago, USA) per O.D.254 unit was added ...
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bioRxiv - Bioengineering 2021Quote: ... a Sephadex G-25 column (GE Healthcare, NAP-5) was equilibrated with PBS (PH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... A HisTrap HP column (5 ml, GE Healthcare, France), prepacked with Ni High Performance Sepharose ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were scanned using a Typhoon 5 (GE Healthcare) and the signal intensity quantified using Image Studio Lite (LI-COR Biosciences).
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bioRxiv - Biochemistry 2022Quote: ... applied to a 5 ml HisTrap HP (GE Healthcare), and washed with lysis buffer using an Äkta pure fast-performance liquid chromatography system (GE Healthcare,) ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare, Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... using a 5 mL HisTrap FF column (GE Healthcare). Bacterial pellets were lysed in a buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... using a SD200 Increase 5/150 column (GE Healthcare) at 0.4 ml/min with 1 frame recorded per second (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... first with HisTrap HP 5 mL columns (GE Healthcare) and next with a size-exclusion HiLoad Superdex 200 PG column (GE healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting sample was applied to a 5 ml HiTrap HP heparin column (GE Healthcare) equilibrated with TGE containing 50 mM NaCl and 1 mM β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and eluted with imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The Mono Q 5/50 GL column (GE Healthcare) was equilibrated with 50 mM Bicine buffer (pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % glycerol on an ÄKTA Pure (GE Life Sciences). Where nucleotide was present ...
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bioRxiv - Molecular Biology 2023Quote: ... Heparin columns (5 ml) were purchased from GE HealthCare or Cytiva.
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bioRxiv - Biochemistry 2024Quote: ... loaded onto 5 ml GST-trap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (GE Healthcare, NA934, NA931), diluted 1:10,000 ...
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bioRxiv - Biochemistry 2019Quote: ... 5% glycerol) to a final NaCl concentration of 20 mM and immediately loaded on a 5 mL HiTrapQ column (GE Healthcare Life Sciences) as a second purification step ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The dissolved ammonium sulfate pellet was loaded on a 5 mL DEAE Sepharose and 5 ml Ni-Sepharose cartridge (GE Healthcare Life Sciences) connected in tandem ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2019Quote: ... The membrane was then incubated with primary antibody diluted in TBST/BSA for one hour followed by a one hour incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10,000, GE Healthcare life sciences, Piscataway, NJ). Primary antibodies used were ...
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bioRxiv - Biophysics 2021Quote: ... Amplified phage particles at 10-fold dilution were assayed against 20 nM biotinylated membrane proteins in nanodiscs using horse radish peroxidase (HRP)-conjugated anti-M13 monoclonal antibody (GE Healthcare, #27-9421-01). Assays were performed in library sorting buffer (200 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3x) with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, dilution 1:10.000) or sheep anti-mouse IgG (GE Healthcare ...