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Citations for GE Life Sciences :
451 - 500 of 3098 citations for Anti Cullin 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HisTRAPTM TALON column (5 mL, GE healthcare) was connected to an AKTA prime chromatography system (GE healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and MonoQ 5/50 GL (GE Healthcare). HsMSH2-MSH6 containing fractions were dialyzed in storage buffer (25 mM HEPES pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... (5) Source 15 RPC resin (GE Healthcare) chromatography using a linear gradient from 0 % to 86 % (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... HiTrapQ (5 ml) column (17115301, GE Healthcare) was equilibrated with the buffer (20 mM Tris-HCl pH 9.5 ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 ml GSH column (GE Healthcare) was mounted after the size-exclusion column ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 ml Talon column (GE Healthcare) was mounted after the size-exclusion column ...
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bioRxiv - Immunology 2021Quote: ... and 5 mL affinity columns (GE Healthcare) on an AktaPure chromatography system ...
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bioRxiv - Biochemistry 2022Quote: ... three 5 mL StrepTrap HP (GE Healthcare) were connected to increase the amount of isolated protein per flow through ...
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bioRxiv - Biochemistry 2022Quote: ... and HiTrap Heparin (5 ml, GE Healthcare) columns that had been equilibrated in tandem with QA buffer ...
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bioRxiv - Physiology 2022Quote: ... supplemented with 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Mono Q 5/50 GL (GE Healthcare); L-α-phosphatidyl-L-serine (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM of each NTP (GE Healthcare), 0.01% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Hyclone FBS (GE Healthcare), 0.5 ng/ml 17 β-oestradiol ...
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bioRxiv - Biophysics 2019Quote: ... and ClpAD1 was purified by ion-exchange chromatography using S-Sepharose and MonoQ HR 5/5 columns (GE Healthcare). Purified ClpAD1 was dialyzed into storage buffer (50 mM HEPES ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_R: 5’-TAGAGGTACCctcgagCTACTCAATGCCGAACGTGTTG-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI WWI_F: 5’-ccgGAATTCCCACCATTGCCGCCTG-3’ CG42797XhoI WWII_R: 5’-ccgCTCGAGTCAACGAGGATCCATGAA-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_Nterm_R: 5’-CCGctcgagTCATTCGCTGGGCTGC-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and incubated in 50 μL per well of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Biochemistry 2019Quote: ... the strips were washed with TBS and incubated with anti-rabbit IgG HRP-conjugated antibodies (RRID: AB_772206, GE Healthcare, Pittsburgh, PA) at a 1:20,000 dilution in TBS + 3% BSA ...
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bioRxiv - Biophysics 2019Quote: ... The remaining lysate was then incubated overnight at 4°C with anti-GFP (Rudner et al., 1999) or anti-SMC (Lindow et al., 2002) antibodies and subsequently incubated by Protein A-Sepharose resin (GE HealthCare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... plaque assays were washed with PBS three times and then incubated for 1 h in anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-tubulin antibody (1:1000, DSHB, University of Iowa, Iowa City, IA, USA) and secondary anti-mouse IgG-HRP (1:2000, GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... and then detected with an anti-rabbit horseradish peroxidase-linked secondary antibody using an ImageQuant LAS 4000 mini digital imaging system (GE Healthcare). Detection of DnaA ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h with 5 μL of anti-MYC antibody (Gentex) followed by 1 h incubation with an additional 50 μL of GammaBind plus Sepharose beads (GE Healthcare) to capture protein complexes ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibody was horseradish peroxidase (HRP)-conjugated anti-mouse IgG or IgA and a chemiluminescence HRP was used as substrate (GE Healthcare). Apparent molecular weight of bands was determined using a standard curve generated from molecular weight markers (r2=0.9916 ...
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bioRxiv - Molecular Biology 2022Quote: Purified NiV F was immobilized to a single flow cell of either (i) a CM5 sensor functionalized with an anti-StreptagII antibody or a NiNTA sensor in a Biacore X100 (GE Healthcare). Binding kinetics for Fabs 4H3 and 1H8 were measured using a CM5 sensor ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... G2 and G1 phases based on the signal intensity of anti-CENPF antibody staining with software (IN Cell Investigator, GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Microbiology 2019Quote: ... clarified lysates were mixed with 1 µg anti-HA monoclonal antibody and rotated with 30 µl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used for immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalogue number NA934) and HRP-conjugated donkey anti-mouse IgG (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...