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Citations for GE Life Sciences :
551 - 600 of 2248 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: The experiments were conducted using a 3-Tesla scanner equipped with an 8-ch phased array coil (GE Healthcare, Signa). Functional images were acquired using a T2*-weighted gradient-echo echo-planar imaging sequence ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were further enriched by centrifugation at room temperature at 695 g for 8 min in 37.5% Percoll (GE Healthcare), prior to washing and resuspension for downstream analysis.
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bioRxiv - Biochemistry 2021Quote: ... and diluted with an equal volume of 50 mM Tris pH 8 before loading onto HiTrap Heparin HP columns (GE Healthcare). After extensive washing with 50 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Biochemistry 2020Quote: ... All products were resolved on 12% polyacrylamide gels containing 8 M urea and visualized using Typhoon Trio fluorescence imager (GE Healthcare).
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biochemistry 2019Quote: SEC-MALS was conducted using a Wyatt Dawn 8+ detector (Wyatt Technology) coupled to a Superdex 75 10/300 column (GE Healthcare) and a refractive index detector ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were centrifuged at 18000 rpm (F21-8×50y rotor) for 45 min and supernatants were purified by Ni2+-affinity chromatography (FPLC system ÄKTA Purifier, GE Healthcare) eluting the protein in 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Molecular Biology 2020Quote: ... The reaction products were resolved on 6–8% (w/v) denaturing polyacrylamide gels and visualized with Typhoon FLA scanner (GE Healthcare). In total ...
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bioRxiv - Microbiology 2021Quote: ... A constant amount of total RNA (between 3.5 and 14 μg) was loaded on 8% urea acrylamide gel in 1X TBE and transferred to a nitrocellulose membrane (GE Healthcare). For detection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples of both male and female livers from all the different treatments (N=6-8) were individually used to isolate total RNA via the Illustra RNAspin Mini RNA Isolation kit (GE Healthcare), according to the manufacturer’s standard protocol ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions were analyzed in a Commasie stained 8% SDS-polyacrylamide gel and gel lanes were scanned using a Typhoon 9400 laser imager (GE Healthcare). Scanned gels were analyzed using ImageQuant TL v2005 software and the two peak fractions were pooled and concentrated to 8.5 mg/ml in 79 μl ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM EDTA (pH 6.8) prior to purification by size exclusion chromatography using a gel filtration column (HiLoad 26/60 Superdex 75 column, GE Healthcare). The sample was concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: Affinity-purified xtZnT8 was concentrated to 6-8 mg/ml and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), and was further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biophysics 2023Quote: ... The NP without MBP containing buffer was diluted to reach 150 mM NaCl concentration by adding 20 mM NaH2PO4 pH 8 and loaded on a heparin column (GE Healthcare) to remove bound bacterial RNA ...
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bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
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bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... the unbound fractions were collected and applied to a Sours 15 Q ion exchange sorbent (column V = 8 mL, GE Healthcare) at a rate of 5 mL/min ...
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bioRxiv - Biophysics 2024Quote: ... pure fractions were buffer-exchanged into the final buffer (20 mM Tris-HCl, pH 8, and 150 mM NaCl) using a HiPrep Desalting column (GE Healthcare) and stored at -20°C in the final buffer ...
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bioRxiv - Immunology 2024Quote: ... Proteins were dialyzed against 10 mM Tris (pH 8) and purified by ion exchange (Q column) followed by size exclusion (S200, GE Healthcare) chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Cancer Biology 2020Quote: Protein expression on immunoblots was quantified on 8-bit gray-scale-transformed .tiff images of either scanned Amersham Hyperfilm MP (GE Healthcare, 28906838) or .tiff images obtained by the Bio-Rad ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of protein (10-50 µg) from the total extract were separated on 8-10% SDS-polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Germany) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at 8-13 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at concentrations >8 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and then inserted immediately upstream of a promoter-less chloramphenicol resistance gene (cat) gene in pBR322 origin a medium-copy-number plasmid pKK232-8 (GE Healthcare, USA) to create the pHL1141 plasmid (8 ...
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Neuroscience 2019Quote: Whole-brain T1-weighted MRI data were acquired using a General Electric HDx 3T MRI scanner and a 8-channel receiver head coil (GE Healthcare, Waukesha, WI) at the Cardiff University Brain Research Imaging Centre with an axial 3D fast spoiled gradient recalled sequence (echo time 3 ms ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... liver and enzyme treated lung were prepared by meshing organs through 70 μm nylon cell strainers in sRPMI-1640 with 8% FBS (HyClone Laboratories, GE Healthcare, Logan, Utah). After red blood cells lysis with NH4Cl (liver ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty micrograms of total RNAs were separated on 15% acrylamide/8 M urea gel and transferred onto an Amersham™ Hybond™-N+ nylon membrane (GE Healthcare). The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham, GE Healthcare Life Sciences). The membrane was blocked in 5% skimmed milk (HIMEDIA ...
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bioRxiv - Cell Biology 2023Quote: ... High resolution images of individual PvLS parasites and PHH nuclei were obtained by capturing 8 planes in the Z dimension using a 100×objective on Deltavision Core (GE Healthcare Life Sciences) and deconvoluted using softWoRx (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... ligation mix was diluted to 50µl and used 0.583:1 volume of home-brewed SPRI beads (SPRI binding buffer: 2.5M NaCL, 20mM PEG 8000, 10mM Tric-HCL, 1mM EDTA, oh=8, 1mL TE-washed SpeedMag beads GE Healthcare, 65152105050250 per 100mL buffer) for purification ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-BrdU (1:1; GE Healthcare, RPN202); mouse anti-PCNA (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... and mixed 1:1 with Percoll (GE Healthcare) to establish a density gradient ...