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Citations for GE Life Sciences :
501 - 550 of 2248 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Neuroscience 2022Quote: ... equipped with an 8-channel receive-only head array coil (GE Healthcare, Milwaukee, WI). First ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions of 8 ml were collected in an automated AKTA FPLC system (GE Healthcare) and purity was determined with SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Cell Biology 2020Quote: ... were activated by 100 mM DTT for 2 hours at room temperature in the dark and then purified using NAP5 columns (GE Healthcare Life Sciences, 17-0853-02) to remove excessive DTT ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... resolved by 8% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare, Munich, Germany) by semi-dry blotting ...
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bioRxiv - Microbiology 2021Quote: ... the dye values were measured using ImageQuant TL version 8 (GE Healthcare, Chicago, IL, USA) and Adobe Photoshop Elements were normalized to density values per mm2 of a worm’s projection area ...
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Rationally designed protein bandpass filters for controlling cellular signaling with chemical inputsbioRxiv - Synthetic Biology 2023Quote: Surface plasmon resonance (SPR) measurements were performed on a Biacore 8 K device (GE Healthcare). The mutual designed binder (LD3 ...
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bioRxiv - Biochemistry 2023Quote: ... 8 μL of the reaction mixture was spot on Amersham Hybond-XL paper (GE healthcare) and dried out ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were purified by centrifugation and filtration through a Whatman 8 µM nuclepore membrane (GE Healthcare) followed by a second filtration step through a 5 µM nuclepore membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were subsequently imaged for 8 hours on a DeltaVision Elite imaging station (GE Healthcare), equipped with a CoolSNAP HQ2 camera ...
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bioRxiv - Bioengineering 2023Quote: ... cored vertebral specimens were scanned using quantitative micro-CT (MS-8, GE Healthcare, London, Ontario, Canada) with 70 kVp X-ray tube peak potential ...
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bioRxiv - Biochemistry 2021Quote: ... cDNAs were separated by 8% TBE-urea gels and visualized by Typhoon™ FLA 9000 (GE Healthcare). Sequencing ladders were made using the Sequenase 2.0 DNA Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated using 8-12% polyacrylamide gels and transferred to Amersham Nitrocellulose Membranes (GE healthcare) via Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were separated by 8% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare, Bucks, UK) using the Trans-Blot SD Semi-Dry Transfer system (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8 supplemented with 0.22 mM DDM by gel filtration using the PD-10 column (GE Healthcare). Samples were concentrated using Amicon®Ultra-4 ultrafiltration device ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were resolved onto an 8% SDS-PAGE and transferred to Protran Premium NC membranes (GE Healthcare). The membranes were blocked with milk (5% [w/v] dried milk in 1X PBST (137mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... CAD was induced for 8 h at 30°C and purified using a HisTrap HP column (GE Healthcare). FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... adjusted to pH 8 with HCl) and applied to an HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) to further separate the low molecular weight contaminants and obtain purified trimers (ÄKTA FPLC, GE Healthcare). Eluted trimer fractions were assessed on the SDS-PAGE gel to determine the purity and selected fractions were pooled and passed through 0.22 µm filter to sterilize the sample ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Biochemistry 2021Quote: ... 0.012% LMNG was concentrated up to 8 mg/ml using a 30 kDa MWCO Vivaspin 20 concentrator (GE healthcare). Extensive optimisation of crystal conditions using sitting-drop and hanging-drop vapour diffusion and other post-crystallisation treatments yielded plate shaped crystals after 3-4 days of incubation at 20°C ...
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bioRxiv - Biochemistry 2020Quote: ... The 100-µl sample (8 mg/ml) was loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with filtered and degassed SEC buffer containing 3% glycerol and 5 mM dithiothreitol instead of MgCl2 and β-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biophysics 2022Quote: ... 180 µL of approximately 8 µM sample was injected onto a Superose6 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM TrisHCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... VRC293 cells were expanded to grow in suspension in serum-free Expi293 media supplemented with 4 mM L-glutamine through a total of 8 passages via 1.0 L and 3.0 L shake flasks and 25 L Wave bioreactor (GE Healthcare) into a Biostat Cultibag STR 200 Plus (Sartorius Stedim Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Digestive fluids were harvested 7-8 days after treatment and sterile filtrated (Whatman syringe filter 0.2 μm, FP 30/0.2 CA-S, GE Healthcare), then stored at −20°C until further use ...
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bioRxiv - Cell Biology 2020Quote: ... Peak TopBP1b6-8 fractions (eluted with 50mM and 75mM imidazole) were desalted (HiTrap™ or HiPrep 26/10, GE Healthcare) in physiological buffer (10mM Na2H/NaH2 PO4 pH 7.6 ...
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bioRxiv - Genomics 2020Quote: ... Equivalent amounts of protein were resolved by 8-10% SDS-PAGE and blotted onto nitrocellulose (0.2 µm, Amersham Protran) with a semi-dry blotter (GE Healthcare). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... T1-weighted anatomical MRI and diffusion-MRI scans were collected from 11 out of the 13 healthy individuals (8 male, 35.2 +/- 12.25 years) on a 3.0 Tesla General Electric Signa Excite HDx (GE Healthcare, Waukesha, WI) clinical MRI system with an eight-channel head receive-only coil ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were run on an 8% SDS-PAGE gel and the protein was then transblotted onto a 0.2 µm nitrocellulose membrane (GE Healthcare), followed by blocking for 3 hours in KPL block (SeraCare ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: Floxed Rasa3 exon three was excised in adult (8-12 weeks of age) hematopoietic cells by inducing Mx1-Cre recombinase activity with Poly(I:C) (GE Healthcare). Mice carrying floxed Rasa3 allele(s ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were electrophoretically separated on an 8% polyacrylamide gel and then wet- transferred to a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare). Prior to antibody incubation ...
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bioRxiv - Bioengineering 2021Quote: ... The UtAs were scanned using the C4-8 transabdominal probe of a GE Voluson E10 (GE Healthcare, Wisconsin, United States) US machine ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 million cells were injected subcutaneously in the flanks of 8 week-old Nude or NSG mice with a ratio of 50/50 standard medium (DMEM: Cat# SH30022.01, GE Healthcare) and BD Matrigel™ (Cat# 356234 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA products were extracted with phenol:chloroform:isoamyl alcohol and analyzed on an 8% urea-PAGE using fluorescence scanner (Typhoon™ FLA7000, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... elution in buffer A plus 350mM imidazole (pH 8) and gel filtration chromatography on a Superdex 200 column (GE Healthcare) in buffer A.
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bioRxiv - Biochemistry 2022Quote: ... Afterwards the lysates were subjected to SDS-polyacrylamide gel electrophoresis using an 8% polyacrylamide gel and then electroblotted onto a nitrocellulose membrane with a pore size of 0.45 μm (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
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bioRxiv - Microbiology 2023Quote: ... elution in buffer A plus 350mM imidazole (pH 8) and gel filtration chromatography on a Superdex 200 column (GE Healthcare) in buffer A.