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Citations for GE Life Sciences :
501 - 550 of 872 citations for IL 1RA Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Extracts (15⍰μg protein) were separated by SDS–PAGE and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked in TBS (150⍰mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Twenty-five µL of Protein A Sepharose beads (50% slurry contained phosphate-buffered saline buffer) (GE healthcare, Chicago, IL) was added to the resultant supernatant ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: MRI scans were performed on each participant using a GE Discovery MR750 3T MRI scanner (GE Healthcare, Chicago, IL). No participant was in the scanner for longer than 120 minutes per session ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was separated using SEC on a Superose 6 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with buffer containing 50 mM Tris-HCl (pH = 7.8) ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrated CM was loaded onto a column packed with Sepharose 4 Fast Flow resin (GE Healthcare, IL, USA). Eluates were measured at 280 nm absorbance to monitor the protein content of each fraction ...
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bioRxiv - Cancer Biology 2019Quote: ... detection was achieved with ECL™ or ECL™ prime western blotting detection reagents (GE Healthcare, Chicago, IL, USA). The antibodies used were ...
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bioRxiv - Bioengineering 2019Quote: ... A 7 mm hole punch was used to cut pieces of blood separation membrane (MF1, GE Healthcare, Chicago, IL) and amplification membrane (0.22 µm PES ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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Structural integrity following focused ultrasound thalamotomy and its correlation with tremor reliefbioRxiv - Neuroscience 2019Quote: All MR imaging studies were performed using the same 3T system (Discovery™ MR750, GE Healthcare, Chicago, IL, USA). Patients were recruited between 2016– 2018 and scanned prior to the procedure and in four follow-up scans ...
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bioRxiv - Bioengineering 2020Quote: ... and the protein:antibody complexes were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2019Quote: ... Whatman® Nuclepore policarbonate filters with pore size of 100 nm were purchased from GE Healthcare (Chicago, IL, USA). Black 96-well plates with transparent bottom (lumox® ...
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bioRxiv - Cell Biology 2021Quote: ... 300 mM KCl was performed using a PD-10 desalting column (GE Healthcare, Chicago, IL, Catalog #17-0851-01). Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... AAV particles was extracted in PBS and purified with an AKTA prime plus chromatography system (GE Healthcare, Chicago, IL) on an AVB Separose HP column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... sdRNA mimics and scrambled controls were ordered as custom miRIDIAN mimics from Dharmacon (GE Healthcare Dharmacon, Inc, Chicago, IL). Mimic and inhibitor sequences are detailed in Supplementary File 5 ...
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bioRxiv - Cell Biology 2021Quote: ... and the signal was captured by a CCD camera (ImageQuant LAS 4000 mini®, GE Healthcare, Chicago, IL, USA). As controls ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Fractions were concentrated and further purified by gel filtration (Superose 6 10/300 GL column, GE Healthcare, Chicago, IL). For the tri-specific MB (298-52-80) ...
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bioRxiv - Genomics 2022Quote: ... 500 pmol of DNA oligonucleotides were immobilized using 20 μl of Streptavidin-Sepharose bead slurry (GE Healthcare, Chicago, IL). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, IL), and equilibrated with SEC buffer (20□mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Molecular Biology 2023Quote: ... washed and dried prior to exposure and signal detection in a Typhoon FLA 9500 (GE Healthcare, Chicago, IL, USA) phosphoimager ...
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bioRxiv - Molecular Biology 2022Quote: ... HRP-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare (Chicago, IL, USA). Doxycycline ...
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bioRxiv - Molecular Biology 2023Quote: ... mice underwent echocardiography with a Vivid iQ R3 Premium and a L8-18i-RS transducer (GE Healthcare, Chicago, IL) under ketamine/xylazine anesthesia ...
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bioRxiv - Immunology 2022Quote: ... Immunoreactive bands were detected using HRP-conjugated secondary antibodies and revealed with ECL substrate (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Developmental Biology 2022Quote: ... Band quantification was performed using the GE FujiFilm ImageQuant LAS-4000 CH mini Imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2023Quote: ... Detection was carried out using peroxidase-conjugated secondary antibodies and the ECL Plus Reagent (GE Healthcare, Chicago, IL, USA) or SuperSignal West Femto (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, Il, USA). T cells were negatively purified with EasySepTM beads (human T-cell isolation kit ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were rinsed in PBST and bound antibodies detected with ECL (GE Healthcare Life Sciences, Chicago, IL, USA).
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracts were separated in SDS-PAGE gels and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Western blotting was performed according to standard protocols ...
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bioRxiv - Microbiology 2024Quote: ... MV samples were administered at six concentrations (1:3 dilutions in Neurobasal A (Hyclone, GE Healthcare, Chicago, IL, USA) containing Glutamax (Hyclone ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Microbiology 2020Quote: ... 200 ml of dense culture was filtered onto a 0.22 μm polyethersulfone Whatman Nuclepore filter (GE Healthcare, Chicago, IL, USA), flash frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... For each IP 100µL chromatin was pre-cleared with BSA blocked protein-G sepharose beads (GE Healthcare, Chicago, IL, USA) and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449 ...
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bioRxiv - Molecular Biology 2021Quote: ... Exposure of the resultant protein bands was performed with an ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare; Chicago, IL).
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and applied to an SEC step using HiLoad 16/60 Superdex 75 (GE Healthcare, Chicago IL) in SEC75 buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated with peroxidase-labeled donkey anti-rabbit IgG (#NA9340-1ML, Ge Healthcare Life Sciences, Chicago, IL, USA) with 5% nonfat dry milk for one hr ...
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bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
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bioRxiv - Biochemistry 2020Quote: ... and 500 μL (4 μg BSA) was injected into an AKTA™ PureL system (GE Healthcare Life Sciences, Chicago IL), and run over a Superdex 200 GL (10/300 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... The dried seeds were placed on two layers of glass microfiber filters (Whatman GF/D, GE Healthcare, Chicago, IL, USA) moistened with distilled water in a Petri dish in the dark at 23 °C for a week (conditioning) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting signal was developed into an image using the Typhoon FLA 9500 (GE Healthcare, Chicago, IL, United States). Densitometry measurements were determined with Image Lab 6.0.1 ...