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Citations for GE Life Sciences :
551 - 600 of 902 citations for IL 1RA Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The membranes were rinsed in PBST and bound antibodies detected with ECL (GE Healthcare Life Sciences, Chicago, IL, USA).
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was carried out using peroxidase-conjugated secondary antibodies and the ECL Plus Reagent (GE Healthcare, Chicago, IL, USA) or SuperSignal West Femto (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... Lysates containing fascin were loaded onto a column packed with glutathione Sepharose 4 FF resin (GE Healthcare, Chicago, IL). Fascin recovered from the glutathione column was cleaved with Prescission protease overnight at 4º C ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Microbiology 2020Quote: ... 200 ml of dense culture was filtered onto a 0.22 μm polyethersulfone Whatman Nuclepore filter (GE Healthcare, Chicago, IL, USA), flash frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... For each IP 100µL chromatin was pre-cleared with BSA blocked protein-G sepharose beads (GE Healthcare, Chicago, IL, USA) and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449 ...
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bioRxiv - Molecular Biology 2021Quote: ... Exposure of the resultant protein bands was performed with an ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare; Chicago, IL).
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and applied to an SEC step using HiLoad 16/60 Superdex 75 (GE Healthcare, Chicago IL) in SEC75 buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated with peroxidase-labeled donkey anti-rabbit IgG (#NA9340-1ML, Ge Healthcare Life Sciences, Chicago, IL, USA) with 5% nonfat dry milk for one hr ...
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bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
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bioRxiv - Biochemistry 2020Quote: ... and 500 μL (4 μg BSA) was injected into an AKTA™ PureL system (GE Healthcare Life Sciences, Chicago IL), and run over a Superdex 200 GL (10/300 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... The dried seeds were placed on two layers of glass microfiber filters (Whatman GF/D, GE Healthcare, Chicago, IL, USA) moistened with distilled water in a Petri dish in the dark at 23 °C for a week (conditioning) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting signal was developed into an image using the Typhoon FLA 9500 (GE Healthcare, Chicago, IL, United States). Densitometry measurements were determined with Image Lab 6.0.1 ...
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bioRxiv - Microbiology 2021Quote: ... Z-stack images were acquired on a DeltaVision Elite High-Resolution imaging system (GE Healthcare Life Sciences, Chicago, IL, USA) using a step size of 0.2 μm with a 60×/l.42 oil objective ...
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bioRxiv - Immunology 2021Quote: ... the protein bands on SDS-PAGE gels were scanned and quantified by laser densitometry (Personal Densitometer SI; GE Healthcare, IL). The densities of the Hoc ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
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bioRxiv - Synthetic Biology 2020Quote: ... The gels were dried and exposed for 14 days on a Storage Phosphor Screen (GE Healthcare Biosciences, Chicago, IL, USA) and imaged with a Typhoon FLA 7000 (GE Healthcare Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Membranes were developed using ECL and images were obtained with ImageQuant LAS 4000 camera system (GE Healthcare, Chicago, IL, USA). Band intensities were quantified using ImageJ software (NIH).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Biosensors selected for further study were purified using a His6 sequence using an ÄKTA Start FPLC (GE Healthcare, Chicago, IL) as previously described (Shivange ...
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bioRxiv - Microbiology 2020Quote: ... and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL)
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bioRxiv - Immunology 2020Quote: ... mononuclear cells were isolated from buffy coats blood using Ficoll-Paque™ PLUS (γ=1.007, GE Healthcare, Chicago, IL, USA). Next ...
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bioRxiv - Cancer Biology 2022Quote: MSLN complex with VH 3C9 was prepared by injecting the protein mixture to Superdex 75 column (GE Healthcare, Chicago IL), and concentrated to 12 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... the cell supernatant was collected and soluble IgG was purified using affinity chromatography (GammaBind Plus Sepharose, GE HealthCare, Chicago, IL). Purified IgG was buffer-exchanged in PBS ...
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bioRxiv - Neuroscience 2022Quote: Biosensors selected for further study were purified via the His6 sequence using an ÄKTA Start FPLC (GE Healthcare, Chicago, IL) as previously described (Shivange et al. ...
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bioRxiv - Physiology 2022Quote: ... M-mode parasternal long axis view was applied using 11.5 MHz transducer (GE 10S-RS, GE Healthcare, Chicago, IL, USA), connected to an echocardiographic imaging unit (Vivid S5 ...
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bioRxiv - Neuroscience 2023Quote: ... Heart rate (pulse oximeter clipped to the distal phalanx) and respiration (mid-thoracic strain gauge; both GE Healthcare, Chicago, IL) were acquired at 50 Hz during each TMS-fMRI run.
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... brains were taken out quickly and rinsed with phosphate buffered saline (PBS) (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 1% fresh protease inhibitor cocktail (EMD Millipore ...
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bioRxiv - Biophysics 2023Quote: ... Fractions containing Aβ42 were lyophilised and further purified using a Superdex 75 HR 26/60 column (GE Healthcare, Chicago, IL), and eluates were analyzed using SDS−polyacrylamide gel electrophoresis for the presence of the desired protein product ...
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bioRxiv - Plant Biology 2024Quote: ... The DNA bands were visualized by fluorescence imaging using a Typhoon Trio Variable Mode Imager (GE Healthcare, Chicago, IL, USA). Analyses of the binding to promoter mutants MA ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µM filter and loaded onto a pre-equilibrated HisTrap HP 5ml column (GE Healthcare, IL, US). The column was washed with 50 ml lysis buffer and eluted with buffer containing 200 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5 ml aliquots of xylem sap were filtered through Sephadex columns (PD SpinTrap® G-25, GE Healthcare, Chicago, Il, USA) using Tris-buffer (50 mM Tris HCl (Roth) ...
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bioRxiv - Cancer Biology 2021Quote: ... filtered using a 0.45 µm Puradisc 25 mm polyethersulfone Syringe Filter (6780-2504, GE Healthcare Life Sciences, Whatman™, Chicago, IL) to remove cellular debris ...