Labshake search
Citations for GE Life Sciences :
5351 - 5400 of 5529 citations for Mouse Anti Hantavirus Nucleocapsid Protein Antibody 4958 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Visualization of the antibody binding sites was achieved using Amersham ECL Prime Western Blotting (GE Healthcare UK Ltd, RPN2232) according to the manufacturer’s protocol.
-
bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...
-
bioRxiv - Immunology 2022Quote: ... Immunoreactive bands were detected using HRP-conjugated secondary antibodies and revealed with ECL substrate (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Biochemistry 2022Quote: ... Bound secondary antibodies detected by chemiluminescence using the ECL kit according to the manufacturer’s instructions (GE Healthcare, Uppsala, Sweden). Finally ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies and associated DNA were then isolated using Gammabind G Sepharose beads (GE Healthcare Bio, catalog # 17-0885-01). Crosslinks were reversed by incubating samples and input at 65°C for at least 6 hours ...
-
bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
-
bioRxiv - Immunology 2023Quote: ... Detection of HRP-linked antibodies was performed using chemiluminescence detection kits (ECL™ or ECL Plus™, GE Healthcare) according to the manufacturer’s instructions and visualized using ChemiDoc™ Touch Imaging System (Bio-Rad Laboratories) ...
-
bioRxiv - Immunology 2022Quote: Antibody Fab fragments were purified further via size-exclusion chromatography using an S200 increase 10/30 column (GE Healthcare) equilibrated with 25 mM Tris (pH 8.0) ...
-
bioRxiv - Neuroscience 2023Quote: ... The membranes were rinsed in PBST and bound antibodies detected with ECL (GE Healthcare Life Sciences, Chicago, IL, USA).
-
bioRxiv - Cell Biology 2023Quote: ... The antibody was affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
-
bioRxiv - Cancer Biology 2023Quote: ... The unconjugated DFO was removed from the antibody-DFO conjugates using a size exclusion column (PD-10; GE Healthcare) and the antibody solutions were concentrated using amicon filters with a 50 kDa cutoff filter ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed with 3X with TBST buffer and incubated with appropriate HRP-conjugated secondary antibodies (GE Healthcare) for 1 hour at RT ...
-
bioRxiv - Developmental Biology 2023Quote: ... The antibodies were affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
-
bioRxiv - Cell Biology 2023Quote: ... Detection was carried out using peroxidase-conjugated secondary antibodies and the ECL Plus Reagent (GE Healthcare, Chicago, IL, USA) or SuperSignal West Femto (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2020Quote: Proteins were finally separated from free fluorophores by size exclusion chromatography with a PD MiniTrap G-25 prepacked columns (GE Healthcare Life Science, Chalfont St Giles, UK). Finally ...
-
bioRxiv - Molecular Biology 2021Quote: ... The EGFP/YFP tags on the proteins was detected by applying SuperSignal® West Dura Extended Duration Chemiluminescent Substrate and imaging with an Amersham Imager 600RGB (GE Healthcare Life Sciences, Little Chalfont, UK).
-
bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
-
bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Where representative blots are shown ...
-
bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
-
bioRxiv - Cell Biology 2019Quote: ... Membranes were washed three times in TBST before incubation with HRP-conjugated secondary antibodies for 1hr at room temperature in 2% milk TBST (1/10,000, GE Healthcare).
-
bioRxiv - Cancer Biology 2020Quote: ... the membranes were incubated with 10,000-fold diluted peroxidase-linked secondary antibody (NA931V for FLAG-BRCA2 and NA934V for beta-Actin; GE Healthcare) at room temperature for 4 hours ...
-
STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare), conjugated to TaBGC1 recombinant protein.
-
bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
-
bioRxiv - Microbiology 2020Quote: ... Biovar-specific LPS antibodies were purified by standard affinity chromatography as described by the manufacturer (GE Life Sciences, PA USA).
-
Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and X-ray film ...
-
bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
-
bioRxiv - Systems Biology 2022Quote: ... Immunoblots were carried out with the antibodies listed above and visualized using the ECL Western Blotting Detection Reagents (GE Healthcare). In order to strip antibodies from the blots ...
-
bioRxiv - Microbiology 2021Quote: ... Wells were washed and probed with 100 μl/well of a goat α-GST antibody (GE Healthcare Life Sciences, 27457701V) diluted 1:800 and incubated for one hour at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... secondary antibodies conjugated with horseradish peroxidase were used for detection by chemiluminescence using the Amersham ECL Prime Kit (GE Healthcare). Biochemical fractionation of infected cells was performed as previously described [19].
-
bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
-
bioRxiv - Immunology 2021Quote: ... extracellular domains were expressed in Drosophila S2 cells and purified by immunoaffinity chromatography with LB3.1 antibody followed by Superdex200 (GE Healthcare) size exclusion chromatography as described [65 ...
-
bioRxiv - Immunology 2020Quote: ... The fractions containing the antibody were pooled and elution buffer was exchanged for PBS using NAP-25 columns (GE Healthcare). Purified VHH72hFc1X7 in PBS was quantified by absorbance at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes incubated with HRP-conjugated secondary antibodies were incubated with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare #RPN2232) for 5 mins ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
-
bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
-
bioRxiv - Plant Biology 2023Quote: ... was used as secondary antibody together with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare; Cat. No. RPN2232) for signal detection.
-
bioRxiv - Physiology 2023Quote: ... the membranes were then washed three times with a PBS 0.3% tween solution and incubated with a horseradish peroxidase-conjugated secondary antibody (GE Healthcare) in PBS 0.3% tween 3% BSA for one hour at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting elutes containing antibodies were applied to a size exclusion chromatography column (Superdex 200 10/300 GL; GE Healthcare) with PBS served as running buffer and a flow rate of 0.5 mL/min (SI Figure 8) ...
-
bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
-
bioRxiv - Biophysics 2022Quote: ... with salt gradient of (250-1000mM NaCl for PARP1 and 50mM-1000mM NaCl for all other constructs) and finally through gel filtration (16/600 200pg or 16/600 75pg, GE Healthcare, depending on the size of the protein) column pre-equilibrated with buffer 150mM NaCl ...
-
bioRxiv - Microbiology 2022Quote: S309-LS and S309-GRLR were produced in ExpiCHO-S cells and affinity-purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61) followed by buffer exchange to histidine buffer (20 mM histidine ...
-
bioRxiv - Neuroscience 2019Quote: ... biotinylated anti-chicken (BA9010, 1:250, Vector Laboratories, CY3-streptavidin (1:500, GE Healthcare). Cell nuclei were counterstained with DAPI (0.2 mg/ml in PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... The anti-GFP nanobody was coupled to Glutathione Sepharose™ 4B beads (GE Healthcare) and incubated with lysates from transfected HEK 293T cells for 1 h at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... and HRP conjugated anti-rabbit IgG (Cat#NA934-1ML, GE Healthcare, 1:50,000 dilution). As a loading control ...
-
bioRxiv - Microbiology 2021Quote: ... membranes were incubated in 1:10000 diluted anti-rabbit IgG (NA934-1ML; GE Healthcare) in TBS-T for one hour at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... the membrane was incubated with an anti-rabbit horseradish peroxidase-linked antiserum (GE healthcare) for 45 minutes ...
-
bioRxiv - Immunology 2019Quote: ... Genentech) was detected with a secondary anti-rabbit IgG conjugated to HRP (GE Healthcare).