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Citations for GE Life Sciences :
5301 - 5350 of 5529 citations for Mouse Anti Hantavirus Nucleocapsid Protein Antibody 4958 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... specific for both primary antibodies and the signal was revealed by ECL Western Blotting Analysis System (GE Healthcare). Additional p-eIF4E ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies were visualized with ECL plus substrate (Pierce, 32132) on a Typhoon FLA9500 (GE Healthcare Life Sciences) for co-IP blots and a ChemiDoc MP Imager (Bio-rad ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; GE Healthcare, Freiburg, Germany). An electrochemiluminescence assay (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... uncoupled dyes were separated from labelled antibodies by size exclusion chromatography (on Sephadex G25 PD10 columns GE Healthcare) followed by dialysis against PBS 1x overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies bound to the PVDF membrane were then detected using enhanced chemiluminescence reagents (GE Healthcare, NJ); the chemiluminescence was digitally imaged and analyzed with the ChemiDocTM MP imaging system and associated software (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with chemiluminescence (34095, Pierce) with a secondary antibody conjugated to Horseradish peroxidase (NXA931V, GE Healthcare). The chemiluminescence signal was obtained by the FUJI Medical X-ray film (4741019289 ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Genetics 2024Quote: ... the membranes were incubated with a horseradish peroxidase-conjugated against the appropriate species antibody (1:10,000; GE Healthcare) diluted in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Blots were then probed with horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, GE Healthcare, NA934, RRID: AB_772206) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC) of the concentrated protein solution was conducted using a HiLoad™ 26/600 Superdex™ 200 pg column (GE Healthcare, Mu-nich, Germany) coupled to the aforementioned chromatography system ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purest form of the proteins were loaded on a size exclusion chromatography HiLoad® 16/60 Superdex® 200 pg column (GE Healthcare Life Sciences / Cytiva), pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with horseradish peroxidase-conjugated donkey anti-rabbit IgG (GE Healthcare). The blots were developed using the ECL system according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated with HRP-conjugated donkey anti-rabbit IgG (GE Healthcare, NA934), diluted 1 ...
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bioRxiv - Genetics 2019Quote: ... and then with horseradish peroxidase-linked donkey anti-rabbit IgG (NA934, GE Healthcare). Blots were stripped and reprobed with rabbit anti-histone H3 antibodes (1:5,000 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with anti-rabbit IgG-HRP (GE Healthcare, NA934V, 1:2000) in TBST for 2 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-rabbit (1:6,000-12,000; GE Healthcare – Life Sciences, ca. no. NA934) secondary antibodies conjugated with HRP and ECL Prime detection reagents were acquired from GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... polyclonal SagA serum and HRP conjugated anti-Rabbit IgG (GE Healthcare, NA 934V) served as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... probed with 1:8,000 of donkey anti-rabbit IgG (GE Healthcare, Piscataway, NJ) or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated IgG F(ab)2 donkey anti-rabbit (GE Healthcare), and HRP-conjugated affinity-purified goat anti-mouse IgG (Jackson).
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bioRxiv - Neuroscience 2020Quote: ... or donkey anti-rabbit-HRP (1:5000, NA934V, GE Healthcare, Chicago, IL, USA) diluted in 1x TBST and incubated for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at room temperature (GE Healthcare, NA934V, donkey anti-rabbit, 1:1000) a 5 min incubation with chemiluminescent HRP substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with donkey anti-rabbit IgG-HRP (1:10,000, GE Healthcare) or sheep anti-mouse IgG-HRP (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... then reacted with goat anti-rabbit immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... followed by reaction with anti-rabbit immunoglobulin G conjugated to HRP (GE Healthcare) at 1:10,000 dilution and visualized by ECL ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated for 1 hour with secondary α-rabbit antibodies conjugated with horseradish peroxidase (HRP) (GE healthcare, NA934) diluted by 1:10,000 in 5% skim milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were washed with TBS-T and incubated with HRP secondary antibodies (GE Healthcare Life Sciences, 1:2000), and immunobands were detected with a Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... before they were incubated with HRP-conjugated secondary antibodies (1:10000, NXA931 and NA934, GE Healthcare Europe, Freiburg, DE) also diluted in blocking solution for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody conjugated with horseradish peroxidase was detected with ECL plus chemiluminescence kit (Amersham Biosciences/GE Healthcare, Piscataway NJ). The images were acquired with an HP Scanjet and densitometry determined using NIH ImageJ ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the filters were incubated with HRP-conjugated secondary antibodies in the respective blocking agent (NA931V and NA934V, GE Healthcare) for 1-2 h at RT or overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... HRP-conjugated secondary antibodies were detected with WesternBright ECL chemiluminescent HRP substrate using a ImageQuant imaging unit (GE Healthcare). Band intensities for reference protein standards and neuropil extracts were calculated in ImageJ ...
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bioRxiv - Cell Biology 2020Quote: ... Signals from HRP-conjugated secondary antibodies were visualized by chemiluminescence (Amersham ECL start Western Blotting Detection Reagent, GE Healthcare) and recorded using the ImageQuant LAS 4000 Imaging System (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... PVDF membranes were washed and revealed using horseradish peroxidase (HRP)-conjugated secondary antibodies and ECL detection reagent (GE Healthcare). The blots were imaged on a Geliance gel imaging systems (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: ... The antibodies were purified on an AKTA FPLC using GE Superdex 200 increase 10/300 GL column (GE HealthCare) in 1× PBS.
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated with secondary antibodies and visualized with the ECL Detection System (GE Healthcare, Little Chalfont, UK).