Labshake search
Citations for GE Life Sciences :
451 - 500 of 1430 citations for N6 2 aminoethyl 9H purine 2 6 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
-
bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
-
bioRxiv - Bioengineering 2020Quote: ... Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™, 300 mg I/ml; GE Healthcare) using a CT-scanner (CT ...
-
bioRxiv - Biochemistry 2023Quote: ... Fractions of 2 ml were collected at a flow rate of 1 ml/min on an ÄKTA prime Plus system (GE Healthcare Biosciences) equilibrated with buffer F ...
-
bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
-
bioRxiv - Molecular Biology 2020Quote: ... 20 µl IgG Sepharose 6 Fast Flow beads (GE Healthcare) were washed twice with 1 ml ice-cold PA-150 buffer [150 mM KCl ...
-
bioRxiv - Biochemistry 2021Quote: ... and finished with SEC (Superose 6 Increase, GE Life Sciences). Recombinant PA28γ was expressed in Rosetta E ...
-
bioRxiv - Biochemistry 2021Quote: ... size-exchange chromatography (SEC) (Superose 6 Increase, GE Life Sciences) was performed on pooled fractions with proteolytic activity after Resource Q ...
-
bioRxiv - Plant Biology 2021Quote: ... Proteins were loaded in a SuperoseTM 6 Increase (GE Healthcare) column ...
-
bioRxiv - Microbiology 2020Quote: ... Affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare Life Sciences ...
-
bioRxiv - Biophysics 2022Quote: ... SUR1core was further purified using Superose 6 increase (GE Healthcare) column running with TBS supplemented with 0.006% GDN ...
-
bioRxiv - Cell Biology 2022Quote: ... before subjecting it to SEC (Superose 6 column, GE healthcare). For labeling purified KASH5 used in TIRF experiments ...
-
bioRxiv - Immunology 2020Quote: ... Size exclusion chromatography using Superose 6 Increase column (GE Healthcare) yielded final purified protein.
-
bioRxiv - Molecular Biology 2020Quote: ... followed by Superose 6 10/300 GL column (GE Healthcare) to a final concentration of 180 μg/ml in PBS.
-
bioRxiv - Immunology 2022Quote: ... and a Superose 6 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Immunology 2022Quote: Purified ORF8N78Q was loaded onto a Superose 6 (GE Healthcare) gel filtration column and eluted in 20 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: A nickel column (Ni Sepharose 6 Fast Flow, GE Healthcare) was washed with deionized water at pH 7.0 ...
-
bioRxiv - Biophysics 2021Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an ÄKTA system in 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2022Quote: ... BLM was further purified using a Superose 6 (GE Healthcare) in buffer D (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biophysics 2022Quote: ... and injected to Superose 6 10/300 column (GE Healthcare) previously equilibrated in size exclusion chromatography (SEC ...
-
bioRxiv - Genetics 2022Quote: ... or IgG Sepharose® 6 Fast Flow Beads (GE Healthcare) was added ...
-
bioRxiv - Biochemistry 2024Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Mes-Tris pH 6.5 ...
-
bioRxiv - Biochemistry 2024Quote: ... using a Superose 6 increase 10/300 column (GE Healthcare) and an Agilent LC-1220 system in 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2024Quote: ... IgG Sepharose 6 Fast Flow beads (20 µl, GE Healthcare) were equilibrated by one wash step using 1 ml of ice-cold TST buffer (50 mM Tris pH 7 ...
-
bioRxiv - Biochemistry 2024Quote: ... A Superose 6 Increase 10/300 GL column (GE Healthcare) was used at 4°C for all SEC purifications ...
-
bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
-
bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
-
bioRxiv - Genomics 2021Quote: ... DNA was stained with 0.2 µg/ml ethidium bromide (EtBr) (Nacalai Tesque, 14631-94) and detected using a Typhoon FLA9000 gel imaging scanner (GE Healthcare, Chicago, Illinois). Gel images were processed using ImageJ 1.8.0 (NIH ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
-
bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
-
bioRxiv - Cell Biology 2021Quote: ... hMBs and C2C12 cells were differentiated in a differentiation induction medium (DIM) consisting of DMEM with 2% horse serum (HyClone; GE Healthcare, UT, USA) and PS (Nihashi et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
-
bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
-
bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
-
bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
-
bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
-
bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
-
bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
-
bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
-
bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
-
bioRxiv - Cell Biology 2024Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
-
bioRxiv - Molecular Biology 2024Quote: ... Reactions were incubated for 2 h at 25°C and then filtered through a Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) on top of a Hybond-XL (Cytiva ...
-
bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...