Labshake search
Citations for Quantifoil Micro Tools :
51 - 100 of 339 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... 3.6 µl of purified protein were applied onto glow- discharged holey carbon-coated grids (Quantifoil R1.2/1.3 Au 300 mesh) and blotted for 3.5 s with a blot force of 0 at 100% humidity and 4 °C before being frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... Vitrified specimen was prepared by applying 3 μl of protein complexes onto a glow-discharged 300 mesh gold holey carbon Quantifoil R 1.2/1.3 grid (Quantifoil Micro Tools) and frozen in liquid ethane cooled by liquid nitrogen inside a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (Quantifoil Instruments, Jena, Germany). Next ...
-
bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
-
bioRxiv - Molecular Biology 2023Quote: ... Aliquots of 3 μL of samples at 0.75 mg/ml or 1.5 mg/ml protein concentration were placed on 300-mesh Cu holey carbon grids (Quantifoil, R1.2/1.3) after glow-discharge (PELCO easiGlow™ ...
-
bioRxiv - Biophysics 2023Quote: ... 3.5 μl of concentrated purified protein was applied to glow-discharged copper holey carbon grids (Quantifoil R 1.2/1.3, 300 mesh). In all cases ...
-
bioRxiv - Cell Biology 2019Quote: ... R 2/1 or R 3.5/1 on 300 mesh Cu/Rh grids (QUANTIFOIL) were glow discharged for 45 s at 25 mA ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cu R2/1 grids (Quantifoil) were plasma cleaned using a NanoClean plasma cleaner (Fischione Instruments ...
-
bioRxiv - Biophysics 2019Quote: ... Quantifoil 200 mesh holey carbon R4/1 or R2/1 copper grids (Quantifoil Micro Tools) were glow discharged for 60 s at 0.2 mbar with 20 mA using a PELCO easiGlow glow discharge system (Ted Pella) ...
-
bioRxiv - Biochemistry 2022Quote: A 3 µL of the protein solutions was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2019Quote: ... an aliquot of 3.5 µl AtMLKL2 or AtMLKL3 protein was applied to a holey carbon grids (Quantifoil Cu 1.2/1.3, 200 mesh) glow-discharged (Harrick Plasma ...
-
bioRxiv - Biochemistry 2022Quote: ... 3 μL of proteins / proteins-phospholipids mix samples were gently deposited on glow discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
-
bioRxiv - Biochemistry 2023Quote: ... An aliquot of 4 μL protein sample of Polθ-HLD-NVB complex at a protein concentration of 0.92 mg/mL was loaded onto a glow-discharged 400 mesh grid (Quantifoil Au R1.2/1.3), blotted with filter paper for 3.0 s and 3 blot force ...
-
bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
-
bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
-
bioRxiv - Biochemistry 2024Quote: ... approximately 3.5 μl of protein solution was deposited onto a Quantifoil R1.2/1.3 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany) coated with a thin carbon film ...
-
bioRxiv - Biochemistry 2022Quote: ... R3.5/1 Cu 200 grids (Quantifoil) were glow-discharged on a glass clover slide for 60 seconds at 25 mA and plunging into liquid ethane was performed using an EM GP (Leica Microsystems) ...
-
bioRxiv - Neuroscience 2020Quote: Holey carbon grids (Quantifoil R2/1) covered with either 2 nm amorphous carbon (Quantifoil ...
-
bioRxiv - Cell Biology 2023Quote: Quantifoil R3.5/1 Au200 grids (Quantifoil) were glow discharged for 30 s in an Auto306 Edwards Turbo Coater at power 6 (20 mA ...
-
bioRxiv - Biochemistry 2020Quote: ... aliquots (3 μL) of the purified protein concentrated to ~ 10 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). For KCC3-DIOA samples ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μL of protein solution at 5 mg/mL (with 0.025% DDM) was applied onto a glow-discharged holey carbon grid (Quantifoil, 300 mesh, R1.2/1.3). Excess samples were blotted for 5.0 s with a blotting force of 3 ...
-
bioRxiv - Biophysics 2021Quote: 3μL of inactive or active CaSR protein was applied to glow-discharged holey carbon 300 mesh grids (Quantifoil Au R12/1.3, Quantifoil MicroTools), respectively ...
-
bioRxiv - Biophysics 2021Quote: ... 2.5 μL of each purified CCKAR–G protein complex was applied individually onto the glow-discharged holey carbon grids (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
-
bioRxiv - Molecular Biology 2023Quote: ... A 2.5-3 μL aliquot of protein sample was dropped onto the glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 0.73 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R2/2, 300 mesh). After blotting for 3 s ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 1.5 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3, 300 mesh). At a temperature of 4°C and under a humidity of 100% ...
-
bioRxiv - Biochemistry 2023Quote: ... 3.0 μL purified protein at a concentration of ∼4.95 mg/mL was used for the glow-discharged holey carbon grids (Quantifoil, R1.2/1.3, Au, 300 mesh). The grids were blotted for 2s at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: The purified apo Polθ-HLD at a protein concentration of 1mg/mL was applied for grid preparation on glow-discharged holey gold grids (Quantifoil Au R1.2/1.3, 400mesh) and GraFuture™ GO grid (Quantifoil Au R1.2/1.3 ...
-
bioRxiv - Biochemistry 2021Quote: UltrAuFoil 0.6/1 300 mesh grids (Quantifoil) were glow discharged in residual air at 25 mA for 3 min using a Pelco EasiGLOWTM ...
-
bioRxiv - Biochemistry 2022Quote: UltrAuFoil® gold grids (0.6/1, Quantifoil) (Russo and Passmore ...
-
bioRxiv - Biochemistry 2023Quote: ... UltrAuFoil 0.6/1 300-mesh grids (Quantifoil) were glow discharged in residual air at 20LmA for 60 s each side using a Pelco EasiGLOW ...
-
bioRxiv - Immunology 2020Quote: An aliquot of 3 μl purified protein of rNLRP1-rDPP9 or rNLRP1FIIND S969A-rDPP9 complex was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh), which were glow-discharged for 30 s at middle level in Harrick Plasma after 2 min evacuation ...
-
bioRxiv - Biochemistry 2023Quote: ... Four microliters of purified Cx36 proteins (4-6 mg/ml) were applied onto a negatively glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Cu 300 mesh). For samples involving Cx36-WT ...
-
bioRxiv - Biochemistry 2021Quote: ... were applied to glow discharged (2.2×10-1 mbar for 20 sec) Quantifoil holey carbon grids (R2/1, 200 mesh, Quantifoil). The grids were plunge vitrified into a liquid ethane/propane mix using a Vitrobot Mark IV at 4 °C and 95% humidity ...
-
bioRxiv - Biochemistry 2020Quote: ... Quantifoil 300 mesh gold grids (either 0.6/1 carbon film, 0.6/1 or 1.2/1.3 UltrAUfoil, Quantifoil Micro Tools GMBH) were glow discharged for 30 seconds prior to use ...
-
bioRxiv - Biochemistry 2019Quote: ... 4 μl of these samples were applied to glow discharged (2.2×10-1 mbar for 2×20sec) Quantifoil holey carbon grids (R2/1, 200 mesh, Quantifoil). The grids were plunge vitrified into a liquid ethane/propane mix using a Vitrobot Mark IV at 4 °C and 95% humidity ...
-
bioRxiv - Biophysics 2023Quote: ... using FluoSpheres (diameter of 1 μm) on gold Quantifoil grids (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
-
bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Plant Biology 2022Quote: 3 μL of purified PSI complex at 1 mg Chl mL−1 were applied to glow-discharged (GloQube Quorum, 40 sec, 20mA) holey carbon grids (Quantifoil 300 Au R1.2/R1.3 ...
-
bioRxiv - Biophysics 2020Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma treated using a Solarus plasma cleaner (Gatan) ...
-
bioRxiv - Plant Biology 2021Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma cleaned using a Solarus plasma cleaner (Gatan) ...
-
bioRxiv - Molecular Biology 2020Quote: ... transferred onto allgold UltrAuFoil R0.6/1 grids (QuantiFoil), and partially hydrogenated following a previously described procedure [17] ...
-
bioRxiv - Molecular Biology 2022Quote: ... The no carbon sample (Quantifoil Cu200 R2/1) was blotted for 1.5 s from the back side right after sample application and immediately plunge frozen in liquid ethane ...
-
bioRxiv - Molecular Biology 2022Quote: R3.5/1 Holey carbon copper grids (QUANTIFOIL, Germany) were glow-discharged by UV light ...
-
bioRxiv - Cell Biology 2023Quote: ... Cu supported 3.5/1 Holey Carbon grids (Quantifoil) were glow discharged on both sides for six seconds each using atmospheric air in order to render them more hydrophilic ...
-
bioRxiv - Cell Biology 2023Quote: ... 200 mesh R2/1 Au EM grids (Quantifoil) were coated with laminin (1:10 dilution ...
-
bioRxiv - Pathology 2023Quote: ... bombycis spores (1×108 spores/mL) were placed on grids (Cu R2/1, 200 mesh) (Quantifoil Micro Tools GmbH, Jena, Germany) for 5 min and germinated in 0.1 M KOH for 15 min at 30°C ...
-
bioRxiv - Microbiology 2023Quote: ... sample were placed onto glow-discharged Quantifoil R2/1 Cu300 holey carbon grids (1 µm spacing, 2 µm holes, copper mesh) (Quantifoil, Germany), blotted for 3.5 s before flash-freezing in liquid ethane using a Vitrobot mark IV (FEI ...