Labshake search
Citations for Quantifoil Micro Tools :
1 - 50 of 339 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... A 3.5 µL virus sample solution was applied onto a glow discharged copper grid coated with holey carbon (R2/1; Quantifoil, Jena, Germany) prior to plunge-freezing ...
-
bioRxiv - Microbiology 2020Quote: ... 3.5 microliters of the purified virus sample was pipetted onto a glow-discharged R2/1 Quantifoil grid (Quantifoil Micro Tools GmbH, Jena, Germany), blotted for 2.5 seconds ...
-
bioRxiv - Molecular Biology 2023Quote: ... The purified virus was applied onto glow-discharged electron microscopy grids covered with holey carbon (Quantifoil, SPT Labtech, Melbourn, UK), blotted ...
-
bioRxiv - Biophysics 2022Quote: A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2023Quote: 4 µL of protein solution (total protein concentration = 1 mg/mL) were applied to freshly glow-discharged QUANTIFOIL® R2/1 300 mesh grids (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 μl of protein sample was applied to holey carbon grids (Quantifoil, R 0.6/1 Cu 200 mesh) which were H2/O2 glow-discharged for 30 s ...
-
bioRxiv - Immunology 2021Quote: ... Then 3 μl of the protein mixture was applied to a Quantifoil-Cu-2/1-3C grid (Quantifoil) pretreated by glow-discharging at 15 mA for 1 min ...
-
bioRxiv - Biophysics 2020Quote: ... 2.5 μl samples of GDN-purified proteins at a concentration of 1.5-3 mg ml−1 were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 or R0.6/1 Au 200 mesh). Excess liquid was removed in a controlled environment (4°C and 100% relative humidity ...
-
bioRxiv - Biophysics 2019Quote: ... 3.5 μl aliquots of the recombinant human TIM22 complex (7 mg ml-1) were dropped onto glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
-
bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
-
bioRxiv - Biochemistry 2024Quote: ... 3.5 μL of this protein-DNA mix were applied to 300-mesh copper R2/1 Quantifoil Holey Carbon Grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 25 mA in a GloQube Glow Discharge System (Quorum Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2.4 μl of 0.12 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2.8 µl of 0.10 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
-
Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: Freshly purified ZorAB sample was concentrated to 2-3 mg/mL and 2.7 μL protein was applied onto the glow-discharged (30 s, 5 mA) grids (Quantifoil R0.6/1 300 mesh Au) and plunge-frozen into liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
-
bioRxiv - Biophysics 2020Quote: ... The protein was spotted onto UltraAuFoil 1.2/1.3 gold grids (Quantifoil) plasma cleaned using an Ar/O2 gas mix ...
-
bioRxiv - Biophysics 2020Quote: ... Protein was frozen on R 1.2/1.3 grids with 300 Au mesh (Quantifoil, Germany). This dataset was collected at UCSF on the Titan Krios 2 ...
-
bioRxiv - Biophysics 2020Quote: The purified N protein was prepared on UltraAuFoil R1.2/1.3 gold support grids (Quantifoil). 3 µl of sample was applied to glow-discharged grids ...
-
bioRxiv - Biophysics 2023Quote: ... by applying protein to glow-discharged QuantiFoil Au R1.2/1.3 200-mesh grids (Quantifoil), blotting for 3.0 s under 100% humidity at 4°C ...
-
bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
-
bioRxiv - Immunology 2021Quote: The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
-
bioRxiv - Biophysics 2020Quote: ... The protein was then blotted onto Cu 300 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) two times with 3.5 μl sample each time ...
-
bioRxiv - Plant Biology 2023Quote: ... 3 μl protein was applied to glow-discharged carbon grids (Quantifoil Cu R1.2/1.3 300 mesh). Subsequently ...
-
bioRxiv - Biophysics 2021Quote: 3 μL aliquots of purified tau filaments from PSP human brain tissue (~0.006 mg/mL) were applied to glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with filter paper and plunge-frozen into liquid ethane using an FEI Vitrobot Mark IV ...
-
bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
-
bioRxiv - Biophysics 2023Quote: 4 μL purified protein was placed on the holy-carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged for 30 s ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μl diluted protein sample was put on freshly glow-discharged Quantifoil R1.2/1.3 Cu grid (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Biophysics 2021Quote: ... 2.0 μL of the protein sample was applied to 300 mesh Cu Quantifoil 1.2/1.3 grids (Quantifoil Microtools) that were glow discharged for 20 seconds at 25 mA ...
-
bioRxiv - Biophysics 2020Quote: ... Aliquots (3.5 μl) of protein solution were loaded onto glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3), which were blotted for 3.5 s and immersed in liquid ethane cooled by liquid nitrogen using Vitrobot (Mark IV ...
-
bioRxiv - Plant Biology 2021Quote: ... 2.5 μl L7TIR protein (1mg/ml) was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh) glow-discharged for 30 s at high level in Harrick Plasma after 2 min evacuation ...
-
bioRxiv - Immunology 2022Quote: ... 3 μL of the DCFHP proteins were applied onto a glow-discharged Quantifoil R 1.2/1.3 Cu 300-mesh grid (Quantifoil). The grids were blotted for 2 sec at 20°C and 100% humidity and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the protein complex was applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). For cryo-EM grids preparation of DHA-FFA4-miniGq complex ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
-
bioRxiv - Biophysics 2021Quote: ... aliquots (3 μL) of the concentrated protein were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3) and blotted for 5 s ...
-
bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of protein to a glow-discharged QuantiAuFoil R1.2/1.3 300-mesh grid (Quantifoil, Germany) and blotted for 2.5 to 4 s under 100% humidity at 4 °C before plunging into liquid ethane using a Mark IV Vitrobot (FEI).
-
bioRxiv - Biophysics 2019Quote: ... 20 nL of the 25 nL eluate containing the purified protein was primed on a 400-mesh copper grid covered with a holey carbon film (R1.2/1.3, Quantifoil, Germany) that was glow discharged for 45 s in air plasma immediately before use ...
-
bioRxiv - Biophysics 2020Quote: Cryo-EM grids were prepared by applying freshly purified channel protein (~3.5 μl) to glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil), which were then blotted for 2 s with ~100% humidity and flash frozen in liquid ethane using FEI Vitrobot Mark IV (FEI) ...
-
bioRxiv - Bioengineering 2020Quote: ... Aliquots (3.3 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV ...
-
bioRxiv - Microbiology 2023Quote: ... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
-
bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...
-
bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
-
bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Biophysics 2021Quote: 3μL of inactive or active CaSR protein was applied to glow-discharged holey carbon 300 mesh grids (Quantifoil Au R12/1.3, Quantifoil MicroTools), respectively ...
-
bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
-
bioRxiv - Microbiology 2022Quote: ... the purified full-length BA.2 spike protein at 4.6 mg/ml in DDM was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (BioShake iQ; Quantifoil Instruments ...
-
bioRxiv - Biophysics 2023Quote: 3.5 µL of protein or lipid solution was applied onto a freshly glow-discharged carbon-coated copper mesh grid (Quantifoil). After 1 min ...