Labshake search
Citations for Quantifoil Micro Tools :
451 - 500 of 736 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
-
bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
-
bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
-
bioRxiv - Biophysics 2019Quote: ... Approximately 5 µL of sample (diluted if necessary) was placed on a copper grid (Quantifoil® S7/2 Cu 400 mesh, holey carbon films, Quantifoil Micro Tools GmbH, Jena, Germany) and any excess liquid was absorbed by a filter paper ...
-
bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
-
bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
-
bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
-
bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
-
bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
-
bioRxiv - Biochemistry 2022Quote: KRASGTPase/BRAFWT/MEK1SASA/14-3-3 complex in SEC buffer was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 400 mesh) and vitrified using a Leica EM GP ...
-
bioRxiv - Microbiology 2024Quote: ... Sample volumes of 3.5 µl (8 OD260 per ml) were applied to grids (Quantifoil, Cu, 300 mesh, R3/3 with 3 nm carbon) which had been freshly glow-discharged using a GloQube (Quorum Technologies ...
-
bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
-
bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
-
bioRxiv - Biophysics 2021Quote: ... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
-
bioRxiv - Molecular Biology 2021Quote: Copper grids with holey carbon support film (R3/3, Quantifoil) and a 2 nm pre-coated continuous carbon layer on top were glow discharged at 2.2 × 10-1 mbar for 20 s ...
-
bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
-
bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... A 3.5 µL virus sample solution was applied onto a glow discharged copper grid coated with holey carbon (R2/1; Quantifoil, Jena, Germany) prior to plunge-freezing ...
-
bioRxiv - Biochemistry 2024Quote: ... 3.5 μL of this protein-DNA mix were applied to 300-mesh copper R2/1 Quantifoil Holey Carbon Grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 25 mA in a GloQube Glow Discharge System (Quorum Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μl was applied to a freshly glow-discharged grid (Quantifoil R0.6/1 Cu 300 mesh grid ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µl of the cell suspension was placed on freshly glow-discharged (for ∼25 s) holey carbon grids (Quantifoil Cu R2/1, 200 mesh). The grids were front blotted with Whatman filter paper and rapidly frozen in liquid ethane ...
-
bioRxiv - Biochemistry 2022Quote: Samples (3 μl) were applied onto a glow-discharged UltrAuFoil grid (Quantifoil GmbH) and were flash-frozen in liquid ethane using the Vitrobot mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: Samples (3 μl) were applied onto a glow-discharged UltrAuFoil grid (Quantifoil GmbH) and were flash frozen in liquid ethane using the Vitrobot mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: ... This sample was used for EM dataset 6: the purified sample was applied to Cu 300 R1.2/1.3 holey carbon grids (Quantifoil) coated with a ∼7 nm homemade carbon film ...
-
bioRxiv - Cell Biology 2020Quote: All samples were imaged on carbon coated 3 mm gold EM finder grids (Quantifoil). Bead test and calibration samples were made by adding 2 µl of 175 nm fluorescent bead solution(Thermo Fisher PS-Speck) ...
-
bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples (3 μl) were applied to Quantifoil 1.2/1.3 Cu 300 holey-carbon grids (Quantifoil) glow-discharged 60 s using a PELCO glow-discharge system (Ted Pella) ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh ...
-
bioRxiv - Biophysics 2019Quote: ... 3 µL of the mixture was applied to glow discharged R1.2/1.3 400 mesh grids (Quantifoil). Grids were blotted with filter paper 494 (VWR ...
-
bioRxiv - Molecular Biology 2019Quote: ... The sample (3 µl) was applied to glow discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil). Grids were blotted for 1.5 s at 16° C or 22° C and ∼100 % humidity and plunge-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Physiology 2021Quote: ... 3 µL of the vortexed dispersion were placed on glow discharged carbon-coated grids (Quantifoil, D) for 1 minute ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μl of the sample was applied to a hydrophilized grids (Quantifoil, R1.2/1.3+2nm carbon) with an additional 2 nm layer of amorphous carbon ...
-
bioRxiv - Plant Biology 2023Quote: ... 3 μl protein was applied to glow-discharged carbon grids (Quantifoil Cu R1.2/1.3 300 mesh). Subsequently ...
-
bioRxiv - Molecular Biology 2020Quote: 3 μL of sample was applied onto a holey carbon grid (Quantifoil, Cu, R1.2/1.3, 300 mesh) rendered hydrophilic by a 30 s glow-discharge in air (11 mA current with PIB-10) ...
-
bioRxiv - Plant Biology 2021Quote: Concentrated PSII solution (3 µl) was applied on glow-discharged holey carbon grids (Cu Quantifoil R1.2/1.3) that were vitrified for cryo-EM structural determination using a Vitrobot FEI (3 s blot at 4°C and 100% humidity) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The sample (3 μL) was applied to freshly glow-discharged Au 300 mesh R1.2/1.3 grids (Quantifoil) in a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biophysics 2021Quote: ... 3 μL was applied to Ultrafoil grids (R1.2/1.3, Au/Au, 300 mesh, Quantifoil Micro Tools GmbH) that had been glow-discharged for 2 min using a Pelco easiGlow (Ted Pella ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Samples (3 µL) were applied to glow-discharged Quantifoil R1.2/1.3 Cu/Rh 200 mesh grids (Quantifoil) (M4R-Gi1-Ipx and M4R-Gi1-Ipx-LY298 ...
-
bioRxiv - Biophysics 2023Quote: ... 3 µL sample was applied to a freshly glow-discharged UltrAuFoil R1.2/1.3 300 mesh grids (Quantifoil), blotted with Whatman No ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μl diluted protein sample was put on freshly glow-discharged Quantifoil R1.2/1.3 Cu grid (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
-
bioRxiv - Microbiology 2021Quote: ... 3 μl of each sample was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using a PELCO easyGLow™ Glow Discharge Cleaning System (Tedpella) ...
-
bioRxiv - Immunology 2022Quote: ... 3 μl of sample was then dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 s at 20 mA ...
-
bioRxiv - Biochemistry 2022Quote: ... 3 µl of TIR-SAVED complex was applied to Holey Carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh), glow discharged for 45 s at current of 45 mA in a EMITECH K100X glow discharger ...
-
bioRxiv - Molecular Biology 2020Quote: A 3 μL sample solution was applied to a Quantifoil holey carbon grid R1.2/1.3 Mo 200 mesh (Quantifoil Micro Tools GmbH ...