Labshake search
Citations for Quantifoil Micro Tools :
451 - 500 of 730 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2019Quote: 3.5 μl of sample of fragmented doublets (~4 mg/ml) or the Sarkosyl A-tubule (~500 μg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biophysics 2021Quote: ... with 12-nm-thick holey R1/4 and R1.2/20 SiO2 film (Quantifoil) were used for the experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh grid, SiO2, Quantifoil Micro Tools) were glow-discharged and UV irradiated for 30 min for sterilization before being immersed in cell culture medium in 35-mm IBIDI μ-Dish ...
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bioRxiv - Synthetic Biology 2023Quote: 4 µl of sample was adsorbed onto holey carbon-coated grid (Quantifoil, Germany) that was glow-discharged ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biophysics 2020Quote: ... 4 µl sample was applied to each freshly glow-discharged grid (Quantifoil, R1.2/1.3). The grids were then plunge-frozen in liquid ethane ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Cell Biology 2019Quote: ... 4 µl of the solution was applied to a glow-discharged (30 s at −35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). After removing excess liquid by blotting the grid from the back side with Whatman filter paper No ...
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bioRxiv - Microbiology 2019Quote: ... Four μl of this mixture was applied onto freshly glow-discharged copper R2/2 of th0 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Using an FEI Vitrobot Mark IV ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 5 µL of sample (diluted if necessary) was placed on a copper grid (Quantifoil® S7/2 Cu 400 mesh, holey carbon films, Quantifoil Micro Tools GmbH, Jena, Germany) and any excess liquid was absorbed by a filter paper ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μl of sample was applied to glow discharged Quantifoil 300 mesh 1.2/1.3 (Quantifoil) grids at 4 °C and 90-100% humidity ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of this sample was then applied to plasma-cleaned holey carbon grids (Quantifoil) and plunge frozen with an FEI vitrobot mark IV at 4°C and 100% humidity.
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bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
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bioRxiv - Microbiology 2021Quote: ... and 5 μl of the washed cells were introduced to Au grids (R2/1, 200 mesh; Quantifoil). The grids were fixed in 4% PFA (in TBS ...
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bioRxiv - Biochemistry 2020Quote: ... 4 µL of sample was applied to Quantifoil Cu R 1.2/1.3 grids (Quantifoil Microtools GmbH) which has undergone a plasma discharge and were vitrified in liquid ethane using a Vitrobot MkIV plunge freezer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Aliquots of 4 µl were applied to glow-discharged 300 mesh R1.2/1.3 Au grids (Quantifoil) using a Vitrobot Mark VI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Each glow-discharged Quantifoil R2/4 200 mesh copper grid (Quantifoil Micro Tools GmbH, Jena, Germany) was then deposited with 4 µL sample and plunge-frozen in liquid ethane using a Vitrobot Mark IV (TFS ...
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bioRxiv - Biophysics 2023Quote: ... 4 μL of Kapβ2•HNRNPH2 was applied to a 300 mesh copper grid (Quantifoil R1.2/1.3) that was glow-discharged using a PELCO easiGlow glow discharge apparatus at 30 mA/30 s on top of a metal grid holder (Ted Pella) ...
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bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Biophysics 2019Quote: ... Perforated carbon film-covered microscopical 200 mesh grids (R1/4 batch of Quantifoil, MicroTools GmbH, Jena, Germany) were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems ...