Labshake search
Citations for Quantifoil Micro Tools :
351 - 400 of 784 citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or 0.5 mg/ml of RanGTP•selinexor-XPO1•ASB8ΔN16•ELOB/C supplemented with 2-fold excess ASB8ΔN16-ELOB/C and selinexor) was applied to a 400-mesh copper grid (Quantifoil R1.2/1.3). For unliganded XPO1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of the sample was applied onto freshly glow-discharged R2/1 carbon grids (Quantifoil), followed by grid blotting for 4 s and plunging into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −15 and plunge-frozen in liquid ethane.
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −15 and plunge-frozen in liquid ethane.
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of protein sample was applied to holey carbon grids (Quantifoil, R 0.6/1 Cu 200 mesh) which were H2/O2 glow-discharged for 30 s ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of purified PP2A-PME-1 complex was applied onto a glow-discharged holey carbon grid (Quantifoil 300 mesh R 1.2/1/3 with ultrathin carbon ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −20 and plunge-frozen in liquid ethane.
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bioRxiv - Physiology 2023Quote: ... Sample solution (~ 3 μl) was applied on freshly glow discharge TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... aliquots of 3 μl were applied to glow discharged grids (R2/1 or R1.2/1.3, Au 300, Quantifoil), blotted for 2 s and immediately plunge-frozen in liquid ethane using an FEI Vitrobot IV at 4°C and 100 % humidity ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µl of sample was applied onto a freshly glow- discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force -15 and plunge-frozen in liquid ethane using the Vitrobot Mark IV system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 16h prior to trypsinization and seeding onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools), which were placed in 3D printed grid holders [39] ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of a 5 μl sample was applied to a glow-discharged holey carbon grid (Quantifoil R2/2, Au, 400 mesh). After waiting for 60 s ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Biophysics 2022Quote: A volume of 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Neuroscience 2022Quote: Primary cultured neurons were seeded onto holey gold EM grids (Quantifoil R 1/4 gold 200 mesh). The fiducial marker solution was prepared as for neocortical synaptosomes ...
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bioRxiv - Cell Biology 2024Quote: ... Quantifoil Holey SiO2 R 1/4 Au 200 mesh EM grids (Quantifoil Micro Tools #N1-s13nAi20-01) were glow discharged on both sides in a Pelco easyglow for 45 s at 0.37 mBar and 15 mA ...
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bioRxiv - Biophysics 2022Quote: Purified wt or mutant D263A or F55A MVMp capsid samples (5 μl) were applied onto R2/2 300 mesh copper grids (Quantifoil Micro Tools, Germany) and vitrified using a Leica EM CPC cryofixation unit ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... intact MVKCg1 yeasts cells resuspended in PBS were vitrified on Quantifoil Au R2/2 London Finder 300-mesh grids (Quantifoil Micro Tools GmbH) following a similar vitrification procedure as described above for crude membranes ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 3.5 μl of the crude membrane sample was deposited onto freshly glow-discharged Quantifoil R2/2 200-mesh grids (Quantifoil Micro Tools GmbH,) using the Leica EM GP (Leica Microsystems ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 3.5 μl of sample was applied to freshly glow-discharged copper Quantifoil R2/2 200-mesh London Finder grids (Quantifoil Micro Tools GmbH) and then plunge frozen using a Leica EM GP plunger (Leica Microsystems ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 uL of the concentrated sample was applied to freshly glow-discharged R2/1 holey carbon grids (Quantifoil), blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... at 13 °C and 100% humidity: 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −20 and plunge-frozen in liquid ethane.
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bioRxiv - Biochemistry 2020Quote: ... A volume of 3 μL of the sample was applied to a glow-discharged grid (Quantifoil R2/1 300 mesh) at 25 °C and 90 % humidity ...
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bioRxiv - Cell Biology 2021Quote: ... 3 µl of the samples at 1 µg/µl was applied to UltrAUfoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH). Grids were glow-discharged for 30 sec at 15 mA prior to sample application (PELCO easiGlow™ ...
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bioRxiv - Biochemistry 2022Quote: 2.8 µl of F-actin sample (3 – 16 µM) was pipetted onto a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). After incubating for 1 – 2 seconds ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 μl samples of GDN-purified proteins at a concentration of 1.5-3 mg ml−1 were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 or R0.6/1 Au 200 mesh). Excess liquid was removed in a controlled environment (4°C and 100% relative humidity ...
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bioRxiv - Biophysics 2022Quote: UltraAUfoil R1.2/3 (Quantifoil Micro Tools GmbH) grids were glow-discharged on both sides for 30 s at 15 mA using a Pelco Easiglow ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biophysics 2024Quote: A 3 μL of aliquot Msmeg MmpL3 ΔC NDs at a concentration of 0.6 mg/mL was applied to glow-discharged (Quantifoil Cu R2/2, 300 mesh) holey carbon grids for 30 sec and blotted for 2×5 sec at 4°C and 100% humidity ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...